LOCUS       sequence-222967-       11832 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C. thermophilum DASH/Dam1 complex (construct used for cryo-EM 
            structure determination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Jenni S, Harrison SC
  TITLE     Structure of the DASH/Dam1 complex shows its role at the yeast 
            kinetochore-microtubule interface.
  JOURNAL   Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436.
  PUBMED    29724956
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-4- 4"; volume: "360"; issue: 
            "6388"; pages: "552-558"
COMMENT     Sequence Label: jk089_h12
FEATURES             Location/Qualifiers
     source          1..11832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    51..70
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    191..210
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        283..301
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             333..355
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             537..560
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             576..599
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             615..638
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      675..722
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    795..814
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     836..852
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    903..922
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        995..1013
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1045..1067
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1444..1491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1564..1583
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    1672..1691
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        1764..1782
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1814..1836
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2564..2581
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2618..2665
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    2738..2757
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    2846..2865
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2988..3010
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3222..3269
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3342..3361
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3450..3469
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        3542..3560
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3592..3614
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3841..3888
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3961..3980
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4093..4112
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(4183..4205)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4291..4430
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4346..4365
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4445..4462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4616..5204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4696..4715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5235..5274)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(6139..6230)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    6278..6311
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6491..7285)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQLTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6602..6621)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7212..7231
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    8013..8032
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8161..8180
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8253..8271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8303..8325
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      8585..8632
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    8705..8724
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     8746..8762
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8813..8832
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8905..8923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8955..8977
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      9222..9269
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    9342..9361
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    9450..9469
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        9542..9560
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             9592..9614
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      9940..9987
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    10060..10079
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    10168..10187
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        10260..10278
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             10310..10332
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      10829..10876
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    10949..10968
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11057..11076
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        11149..11167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             11199..11221
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      11496..11543
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    11616..11635
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11748..11767
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11799..11832
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggggtaccgc ggccgcgtag aggagatgta ggtgttggca ccggccggcc aattgtgagc
       61 gctcacaatt actcgagtat gcataactat aacggtccta aggtagcgac ctaggttcta
      121 gacttcattt ccttctccag attaactcaa ttccagcatt tagaacccta ccaagtatcg
      181 atggccggcc aattgtgagc gctcacaatt tcgagctctg agccctactg tcgagaaata
      241 tgtatctcgc actcgagtgg cgcgccctcg atcccgcgaa attaatacga ctcactatag
      301 ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa ggagatatac
      361 atatgtcgta tttcgagcag cagcgccagg cgctgataga agagattgcc atgaacttcg
      421 agcatgtcct ggccaacata aacaaactaa accggtcgct cgaggccgtt atcgctgtag
      481 gaaatgagtt ctcatctgtc gaggcgctct ggtcgcaatt cgaaaacgtg atgtcttgga
      541 gccatccgca gtttgaaaaa ggcgccatga ccggttggtc ccacccgcag ttcgaaaaac
      601 gtagcgcggg cagctggagt catccgcaat tcgaaaaata attaacctag gctgctgcca
      661 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      721 tgctgaaagg cgccggcggc ggccgcggcc ggccaccagc agcttgccag ctctttcagt
      781 atcatggagc ccataattgt gagcgctcac aattactcga gtcccggggg atcctgtaaa
      841 acgacggcca gtgaattcta gagcaataac ccatcaatct gtacgcacat cgatggccgg
      901 ccaattgtga gcgctcacaa tttcgagctc tgagccctac tgtcgagaaa tatgtatctc
      961 gcactcgagt ggcgcgccct cgatcccgcg aaattaatac gactcactat agggagacca
     1021 caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat acatatggaa
     1081 gcgcgcgaag cagcccttcg caaggagctc gagggcgttc gcaagatcaa cgaggttatc
     1141 gagggcatga tcgggacgct ggagcgggct aaggggaata tgggtaccgt ctcccaaacc
     1201 gtcaccaacg ccacaaccct gctcaacacc tggacgcgca tgctctcgca gaccgagcac
     1261 aaccagcgcc tgatcctcaa cccggaatgg aagggtgcca cgcaggatct gctcgagttg
     1321 gaagcggaag agcgtagaag acaagaggag gctgagcgtc gcgctgcgga ggcagagcgt
     1381 cgtcgggaag aggctcgtcg gaaggcctaa ttaacctagg ctgctgccac cgctgagcaa
     1441 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaaggc
     1501 gccggcggcg gccgcggccg gccaccagca gcttgccagc tctttcagta tcatggagcc
     1561 cataattgtg agcgctcaca attactcgag tcccgggaag cttcgccagg gttttccaag
     1621 tcgatcacct acagattgct agagtatgcc tttccagatc gatggccggc caattgtgag
     1681 cgctcacaat ttcgagctct gagccctact gtcgagaaat atgtatctcg cactcgagtg
     1741 gcgcgccctc gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc
     1801 ctctagaaat aattttgttt aactttaaga aggagatata catatgtcgt tattatcagc
     1861 acacctggag cagatctcca tctcctgcca agggatcgac agtctcccct tcccccctcc
     1921 caaaatcttc accaacgctc ttctctccaa tcccgacatc acctctctaa tccgtgacac
     1981 cgaagcccac gagcgcgccc tcttctctgt cccacctccc ccaccccgcc aaaccactct
     2041 tactgctgaa caacagcaac aacaacagaa gccttccaat cgccggcaaa cagtcttcaa
     2101 tgtcaccggc ggcgagatca ggacaggtgg cgtaggcagc gcatcaaccg cccgccgtaa
     2161 cacggcggtg gcggccgtgc tgggcggcga cctgcacgcg cagatcatgc gggggactcg
     2221 agccaggccg ggacagcagc ctgggtctgg ggatatagat atggaggtgc tgttgagagg
     2281 ggttgagaag ttgtgcgctg tatacccgct gccgggggcg ttggagaggg ttccggtgat
     2341 caggcagaag tggcaggcgc agagtaatac gttggcgtac tatgaggcga agattgctga
     2401 acagcaggag atgttggatc ggattgcgca ggagaggatg atgaatgatg gcgatgggga
     2461 tgtggagatg gaggatgtag aagaggttgg gatgacggag gaggatttga ggagggagga
     2521 ggaggaggta agggagttgg ataaaaggaa gagggatttg cagcatcacc atcatcacca
     2581 ctaattaacc taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     2641 taaacgggtc ttgaggggtt ttttgctgaa aggcgccggc ggcggccgcg gccggccacc
     2701 agcagcttgc cagctctttc agtatcatgg agcccataat tgtgagcgct cacaattact
     2761 cgagtcccgg gggtaccttc tcattcccgg atcacatcac tatctctgta atatggcgtt
     2821 cttctgattc catcgatggc cggccaattg tgagcgctca caatttcgag ctctgagccc
     2881 tactgtcgag aaatatgtat ctcgcactcg agtggcgcgc cctcgatccc gcgaaattaa
     2941 tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt
     3001 aagaaggaga tatacatatg aacgcatttg agccagcatt tgccgagctc gccgatgctg
     3061 tggccgacct cgaagccaac atgatgcact ttcagttgat gcacgaatca ctggcccggt
     3121 tcagcgaaag cttcgccagt tttctctacg gtctgaacat gaacgcgttc tgtgttgatt
     3181 tcccataatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     3241 cctctaaacg ggtcttgagg ggttttttgc tgaaaggcgc cggcggcggc cgcggccggc
     3301 caccagcagc ttgccagctc tttcagtatc atggagccca taattgtgag cgctcacaat
     3361 tactcgagtc ccggggagat ttgtaagagc tgttagttac gtcgctaccc aatgtcgtta
     3421 gtgtaggttc tgagcatcga tggccggcca attgtgagcg ctcacaattt cgagctctga
     3481 gccctactgt cgagaaatat gtatctcgca ctcgagtggc gcgccctcga tcccgcgaaa
     3541 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
     3601 ctttaagaag gagatataca tatggctgtc aaggcgaggc aattggccca tctccactca
     3661 cagctgaccc agctttctca caacttggcc accacagaaa acttgatgcg tatgacagca
     3721 gtacaggctg aagctatgcg aggtcttgga agctggcatg caggactttt catggctgca
     3781 agcaaggttc ttggagagga gtcgtaatta acctaggctg ctgccaccgc tgagcaataa
     3841 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggcgcc
     3901 ggcggcggcc gcggccggcc accagcagct tgccagctct ttcagtatca tggagcccat
     3961 aattgtgagc gctcacaatt actcgagtcc tgggctcgag agatccggct gctaacaaac
     4021 ccgcttaagg aagatggcca acttatttac ataaggctgc tgccaccgct gagcaataac
     4081 tagcggcgcg ccaattgtga gcgctcacaa ttcgccggcg gtttaaaccc atctaattgg
     4141 gaattccccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     4201 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     4261 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     4321 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4381 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     4441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4621 ataggttccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5221 tttctaccgg tccgtccaaa aaaaaaggct ccaaaaggag cctttaattg tatcggttta
     5281 ccaatgctta atcagtgaag cccctatttc agcaatctgt ctatttcgtt cgtccatagt
     5341 tgcctgacta cccgtcgtgt agataactac gatacgggaa ggcttaccat ctggtccaag
     5401 tgcggcaatg ataccccgtg aaccacgctc tcctgcccca gatttatcag caataaacca
     5461 gccagcagga agtgccgaac gcagtagtgg tcctgcaact ttatccgctt caagccagtc
     5521 tattaattgt tgccgtgaag ccagagtaag tagttcgcca gttaatagtt tgcgtaatgt
     5581 tgttgccatt gccgcaggca ttgtggtgtc tcgctcgtcg tttggtatgg cttcattcag
     5641 ttctggttcc caacggtcaa ggcgagttac atggtctccc atattgtgca aaaaagcggt
     5701 taattccttc ggtccaccga tggttgtcag aagtaagttc gccgcagtgt tatcactcat
     5761 cgttatggcg gcactacata attctcttac cgtcattccg tccgtaaggt gcttttctgt
     5821 cactggggag tattcaacca agtcattctg cgaatagtgt attcgtcgtc cgagttgctc
     5881 ttgccccgca tctacacggg ataatactgc tccacatagc agaactttga aagtgctcat
     5941 cattggaaaa cgctcttcag ggcgaaaact ctcaaggatt ttaccgctat tcaagtccag
     6001 ttctatgtat cccactcgtg cccccaattg gtcctctgcg tcttttactt tcacgagcgt
     6061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggaatt
     6241 aaaaaaaagc ccgctcatta ggcgggctac tagtcagata acttcgtata atgtatgcta
     6301 tacgaagtta tgggtacgta gtgggcgaag aactccagca tgagatcccc gcgctggagg
     6361 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     6421 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     6481 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     6541 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     6601 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     6661 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     6721 catgtgtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     6781 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     6841 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     6901 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     6961 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     7021 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     7081 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     7141 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     7201 ttgtcagttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     7261 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
     7321 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     7381 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
     7441 aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
     7501 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     7561 gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt
     7621 gcttgcggca gcgtgaagct aattcccatg tcagccgtta agtgttcctg tgtcactcaa
     7681 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
     7741 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
     7801 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
     7861 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
     7921 atgagrgytt agtwcgttar acatgagagc ttagtacgtt aaacatgaga gcttagtacg
     7981 tgaaacatga gagcttagcc cgggggccgg ccaattgtga gcgctcacaa ttactcgagt
     8041 atctatgtcg ggtgcggaga aagaggtaat gaaatggcac ctaggttcta gacttcattt
     8101 ccttctccag attaactcaa ttccagcatt tagaacccta ccaagtatcg atggccggcc
     8161 aattgtgagc gctcacaatt tcgagctctg agccctactg tcgagaaata tgtatctcgc
     8221 actcgagtgg cgcgccctcg atcccgcgaa attaatacga ctcactatag ggagaccaca
     8281 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatggagag
     8341 ccctcacgag catcagcaga acttgctgct gtcgcgtatc atcacgaatg tggagaagct
     8401 caacgaggcc atcatggtga tgaacaagac ccttcaagaa atcaacatcc agaacatgaa
     8461 cattgaactt gtggcccaga tgtttaagaa ctatcaatcc aacgtcttgt tccatctgga
     8521 ggctaccgat aacttgaagg atccggcata attaacctag gctgctgcca ccgctgagca
     8581 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     8641 cgccggcggc ggccgcggcc ggccaccagc agcttgccag ctctttcagt atcatggagc
     8701 ccataattgt gagcgctcac aattactcga gtcccggggg atcctgtaaa acgacggcca
     8761 gtgaattcta gagcaataac ccatcaatct gtacgcacat cgatggccgg ccaattgtga
     8821 gcgctcacaa tttcgagctc tgagccctac tgtcgagaaa tatgtatctc gcactcgagt
     8881 ggcgcgccct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
     8941 cctctagaaa taattttgtt taactttaag aaggagatat acatatgctc tctccactcg
     9001 agcaagaagt gttggatgag tatgagcggc tatctgagaa catgaagaag ttggctgtgc
     9061 tcttggatga gcttgccagt gccccagcaa ctgagatttt agatgggctg cgcgagttgg
     9121 agcgtaagac tagcctcgtg ttcacattgc ttaaggctag tgtgtacagc atcgtgttgc
     9181 agcagtaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     9241 cctctaaacg ggtcttgagg ggttttttgc tgaaaggcgc cggcggcggc cgcggccggc
     9301 caccagcagc ttgccagctc tttcagtatc atggagccca taattgtgag cgctcacaat
     9361 tactcgagtc ccgggaagct tcgccagggt tttccaagtc gatcacctac agattgctag
     9421 agtatgcctt tccagatcga tggccggcca attgtgagcg ctcacaattt cgagctctga
     9481 gccctactgt cgagaaatat gtatctcgca ctcgagtggc gcgccctcga tcccgcgaaa
     9541 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
     9601 ctttaagaag gagatataca tatgtcgccg gctctgctgg ctcggattaa cgagaagaag
     9661 gctgagctcg agaacctgaa ggagcttcgc gatctcagcg cagctgttgc tgcccagatg
     9721 gaggcacttg agcagaagct ctctactctc tctagcggga ctgaggccat tgccacggtg
     9781 ttggctaatt ggcataatgt gctacgcgcc atcagcatgg catcggccaa gattcccaaa
     9841 ccaaaggagg aaactgagga gaacacggtt cctcttcccc agactctggt ccggattccc
     9901 acctaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     9961 tctaaacggg tcttgagggg ttttttgctg aaaggcgccg gcggcggccg cggccggcca
    10021 ccagcagctt gccagctctt tcagtatcat ggagcccata attgtgagcg ctcacaatta
    10081 ctcgagtccc gggggtacct tctcattccc ggatcacatc actatctctg taatatggcg
    10141 ttcttctgat tccatcgatg gccggccaat tgtgagcgct cacaatttcg agctctgagc
    10201 cctactgtcg agaaatatgt atctcgcact cgagtggcgc gccctcgatc ccgcgaaatt
    10261 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
    10321 ttaagaagga gatatacata tgtcttacgc tgattgtgtc tgctctcttc ggacctccct
    10381 cgccttcctc gaatcctcgg tcgcaaccct cgacaacggc gtccaagact tcccccgcct
    10441 gtgccacgtc ctcagaaccg tgcgtcacta cgaactaatc ccccagacaa ccctcgccgc
    10501 cgcagaagct tccctccgcg acgaaatcgg cccgtttatc cagctccttc tcgaccgtgc
    10561 cgagaagcat ctagatcgcc aggcaaggag gatagaaacc ctcaaggcgc gcgcagaact
    10621 caacgcaggg agattgtcgc agtactctgg cgacggacac aacaatggga aattctctgg
    10681 tcaggggatg gatttcagga agagtcgtcc gctgaatggg gaggcggctc tgagggccaa
    10741 agttgtaagg cagaggaagg aggcgttaaa gtatagtgtt gagaggctgg agtaattaac
    10801 ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
    10861 cttgaggggt tttttgctga aaggcgccgg cggcggccgc ggccggccac cagcagcttg
    10921 ccagctcttt cagtatcatg gagcccataa ttgtgagcgc tcacaattac tcgagtcccg
    10981 gggagatttg taagagctgt tagttacgtc gctacccaat gtcgttagtg taggttctga
    11041 gcatcgatgg ccggccaatt gtgagcgctc acaatttcga gctctgagcc ctactgtcga
    11101 gaaatatgta tctcgcactc gagtggcgcg ccctcgatcc cgcgaaatta atacgactca
    11161 ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag
    11221 atatacatat gaccctgacg gaagagcttg aaaagctcga gcagcagatt accctcacgc
    11281 tgcaagagat cgactccaac tttgccaaag cccaccgcat cgtcactacg agcatcctgc
    11341 cactcgtcga gcagtatggc gagcactcac gcgcagtgtg ggaggcgacc aagttttgga
    11401 aacaattctt cgaagcctcc gcaaacgtct ctctctccgg ctacgaagag ctagttgact
    11461 aattaaccta ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
    11521 aacgggtctt gaggggtttt ttgctgaaag gcgccggcgg cggccgcggc cggccaccag
    11581 cagcttgcca gctctttcag tatcatggag cccataattg tgagcgctca caattactcg
    11641 agtcccgggc tcgagagatc cggctgctaa caaacccgct taaggaagat ggccaactta
    11701 tttacataag gctgctgcca ccgctgagca ataactagcg gcgcgccaat tgtgagcgct
    11761 cacaattcgc cggcggttta aacccatgtg cctggcagat aacttcgtat aatgtatgct
    11821 atacgaagtt at
//
