LOCUS       sequence-22298-e         751 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Nobes CD, Hall A
  TITLE     Rho GTPases control polarity, protrusion, and adhesion during cell 
            movement.
  JOURNAL   J Cell Biol. 1999 Mar 22. 144(6):1235-44.
  PUBMED    10087266
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1999 Mar 22. 144(6):1235-44."
COMMENT     Sequence Label: pRK5myc Rac1 L61
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..87
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(97..273,274..276,277..672)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattggc caccatggag
       61 cagaagctga tctccgagga ggacctggga tccccgcagg ccatcaagtg tgtggtggtg
      121 ggagacggag ctgtaggtaa aacttgccta ctgatcagtt acacaaccaa tgcatttcct
      181 ggagaatata tccctactgt ctttgacaat tattctgcca atgttatggt agatggaaaa
      241 ccggtgaatc tgggcttatg ggatacagct ggactagaag attatgacag attacgcccc
      301 ctatcctatc cgcaaacaga tgtgttctta atttgctttt cccttgtgag tcctgcatca
      361 tttgaaaatg tccgtgcaaa gtggtatcct gaggtgcggc accactgtcc caacactccc
      421 atcatcctag tgggaactaa acttgatctt agggatgata aagacacgat cgagaaactg
      481 aaggagaaga agctgactcc catcacctat ccgcagggtc tagccatggc taaggagatt
      541 ggtgccgtaa aatacctgga gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt
      601 gacgaagcga tccgagcagt cctctgcccg cctcccgtga agaagaggaa gagaaaatgc
      661 ctgctgttgt aagaattcct gcagaagctt ggccgccatg gcccaacttg tttattgcag
      721 cttataatgg ttacaaataa agcaatagca t
//
