LOCUS       sequence-223028-        2656 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GoldenBraid2.0 Omega 1 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: Omega 1
FEATURES             Location/Qualifiers
     source          1..2656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..79
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        133..151
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     133..150
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    158..214
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(216..235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(217..235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(242..259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(242..258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(467..486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(623..1211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(703..722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1326..1428
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1429..2088
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1495..1514)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2201..2629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2288..2307)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2498..2519
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatcc ggtctcagga gagagacgcc tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgaccatga
      121 ttacgccaag ctatttaggt gacactatag aatactcaag cttgcatgcc tgcaggtcga
      181 ctctagagga tccccgggta ccgagctcga attcgcccta tagtgagtcg tattacaatt
      241 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      301 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      361 gcccttccca acagttgcgc agcctgaatg gcgaatgacg cctgatgcgg tattttctcc
      421 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      481 atgccgcata gcgtctctcg ctgtcatgag accggatcct gacacggtta tccacagaat
      541 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      601 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      661 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      721 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1021 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1261 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1321 tgtgatgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1381 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1441 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1501 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1561 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1621 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     1681 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1741 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1801 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1861 gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1921 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1981 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     2041 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     2101 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     2161 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     2221 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2281 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2341 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2401 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     2461 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2521 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2581 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2641 ttaacgctta caattt
//
