LOCUS       sequence-223255-        1272 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  CRISPR-mediated repression of GLIS3. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3
            TS...
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        125..200
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        231..741
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     660..678
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             790..975
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 ttgnagtttt aattatgttt taaatggact atcatatgct taccgtaact tgaaagtatt
       61 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcaccg gcgacggaac
      121 ccgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      181 aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa ggggttgggg
      241 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      301 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      361 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      421 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      481 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      541 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      601 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      661 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      721 atcaccgacc tctctcccca ggaccggtga tccgccacca tggatgctaa gtcactaact
      781 gcctggtccc ggacactggt gaccttcaag gatgtatttg tggacttcac cagggaggag
      841 tggaagctgc tggacactgc tcagcagatc gtgtacagaa atgtgatgct ggagaactat
      901 aagaacctgg tttccttggg ttatcagctt actaagccag atgtgatcct ccggttggag
      961 aagggagaag agcccggcgg ttccggcgga gggtcgatgg acaagaagta cagcatcggc
     1021 ctggccatca gaaccaactn ngtcggttgg gccgtgatca ccgacgagta aaggtgccca
     1081 gcaagaaatt caaggtgctg ggnaaactcg accggnncag catcaaaaaa acctgatcgg
     1141 acccngctgt tcaacnnngt ataattagcc gagccacccg gctgaaaaac cgccgaaaaa
     1201 taacccnaac gaaaaancng atcggttatc gnaaaaannt ttccaaacgg attgnccaag
     1261 ggggaataan tt
//
