LOCUS       sequence-223659-        9865 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Confers constitutive expression of KKH SaCas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Shen MW, Arbab M, Hsu JY, Worstell D, Culbertson SJ, Krabbe O, Cassa
            CA, Liu DR, Gifford DK, Sherwood RI
  TITLE     Predictable and precise template-free CRISPR editing of pathogenic 
            variants.
  JOURNAL   Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. 
            Epub 2018 Nov 7.
  PUBMED    30405244
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0686-x"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7733"; pages: "646-651"
COMMENT     Sequence Label: p2T-CAG-KKHSaCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1749..1758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4923..4943
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4950..5015
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5066..5187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5351..5472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5630..6028)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6047..6094)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6142..6471)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6156..6291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6210..6229)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6456..6476
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(6742..6762)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6742..6760)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6781..6797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6781..6797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6794..6816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6805..6821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6829..6835,6836..6853,6854..6859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6874..6895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7012..7029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7183..7771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7263..7282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7942..8733,8734..8802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8565..8584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8803..8907)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8933..9387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9070..9091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9282..9301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9514..9536
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9528..9545
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9529..9545
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9555..9574
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9555..9573
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9803..9812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9818..9851)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggtgg ggcggcggcc aatcagaggg gcgcgctccg aaagtttcct
      601 tttatggcgg ggcgggggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccgggggggg ctcggggggg gggcgcggcg gcccccgggg cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagcggccg
     1741 caccggtcgc caccatgggg aaacggaact acatcctggg gcttgacatt gggataacca
     1801 gcgttggcta cggaattatt gattatgaga cacgcgatgt gattgacgcc ggggttaggc
     1861 tgttcaaaga ggccaacgtt gaaaacaacg agggaagacg gagtaagcgc ggagcaagaa
     1921 gactcaagcg cagacggaga catcggattc agagggtgaa aaagctgctc ttcgattaca
     1981 atctcctgac cgatcatagt gagctgagcg gaatcaaccc ctacgaggcg cgagtgaaag
     2041 ggctttccca gaagctgtcc gaagaggagt tctccgccgc gttgctgcac ctggccaaac
     2101 ggaggggggt tcacaatgta aacgaagtgg aggaggacac gggcaatgaa cttagtacga
     2161 aagaacagat cagtaggaac tctaaggctc tcgaagagaa atacgtcgct gagttgcagc
     2221 ttgagagact gaaaaaagac ggcgaagtac gcggatctat taataggttc aagacttcag
     2281 attacgtaaa ggaagccaag cagctcctga aagtacagaa agcgtaccat cagctcgatc
     2341 agagcttcat cgatacctac atagatttgc tggagacacg gaggacatac tacgagggcc
     2401 caggggaagg atctcctttt gggtggaagg acatcaagga atggtacgag atgcttatgg
     2461 gacattgtac atattttccg gaggagctca ggagcgtcaa gtacgcctac aatgccgacc
     2521 tgtacaatgc cctcaatgac ctcaataacc tcgtgattac cagggacgag aacgagaagc
     2581 tggagtacta tgaaaagttc cagattatcg agaatgtgtt taagcagaag aagaagccga
     2641 cacttaagca gattgcaaag gaaatcctcg tgaatgagga agatatcaag ggatacagag
     2701 tgacaagtac aggcaagccc gagttcacaa atctgaaggt gtaccacgat attaaggaca
     2761 taaccgcacg aaaggagata atcgaaaacg ctgagctcct cgatcagatc gcaaaaattc
     2821 ttaccatcta ccagtctagt gaggacattc aggaggaact gactaatctg aacagtgagc
     2881 tcacccaaga ggaaattgag cagatttcaa acctgaaagg ctacaccggg acgcacaatc
     2941 tgagcctcaa agcaatcaac ctcattctgg atgaactttg gcacacaaat gacaaccaaa
     3001 ttgccatatt caaccgcctg aaactggtgc caaaaaaagt ggatctgtca cagcaaaagg
     3061 aaatccctac aaccttggtt gacgatttta ttctgtcccc cgttgtcaag cggagcttca
     3121 tccagtcaat caaggtgatc aatgccatca ttaaaaaata cggattgcca aacgatataa
     3181 ttatcgagct tgcacgagag aagaactcaa aggacgccca gaagatgatt aacgaaatgc
     3241 agaagcgcaa ccgccagaca aacgaacgca tagaggaaat tataagaaca accggcaaag
     3301 agaatgccaa gtatctgatc gagaaaatca agctgcacga catgcaagaa ggcaagtgcc
     3361 tgtactctct ggaagctatc ccactcgaag atctgctgaa taatccattc aattacgagg
     3421 tggaccacat catccctaga tccgtaagct ttgacaattc cttcaataac aaagttctgg
     3481 ttaaacagga ggaaaattct aaaaaaggga accggacccc gttccagtac ctgagctcca
     3541 gtgacagcaa gattagctac gagactttta agaaacatat tctgaatctg gccaaaggca
     3601 aaggcaggat cagcaagacc aagaaggagt acctcctcga agaacgcgac attaacagat
     3661 ttagtgtgca gaaagatttc atcaaccgaa accttgtcga tactcggtac gccacgagag
     3721 gcctgatgaa tctcctcagg agctacttcc gcgtcaataa tctggacgtt aaagtcaaga
     3781 gcataaatgg gggattcacc agctttctga ggagaaagtg gaagtttaag aaggaacgaa
     3841 acaaaggata caagcaccat gctgaggatg ctttgatcat cgctaacgcg gactttatct
     3901 ttaaggaatg gaaaaagctg gataaggcaa agaaagtgat ggaaaaccag atgttcgagg
     3961 agaagcaggc agagtcaatg cctgagatcg agacagagca ggaatacaag gaaattttca
     4021 tcacccctca tcagattaaa cacataaagg acttcaaaga ctataaatac tctcataggg
     4081 tggacaaaaa acccaatcgc aagctcatta atgacaccct gtactcaaca cggaaggatg
     4141 ataaaggtaa taccttgatt gtgaataatc ttaatggatt gtatgacaaa gataacgaca
     4201 agctcaagaa gctgatcaac aagtctccag agaagctcct tatgtatcac cacgacccac
     4261 agacttatca gaaattgaaa ctgatcatgg agcaatacgg ggatgagaag aacccactct
     4321 acaaatatta tgaggaaaca ggtaattacc tgaccaagta ctccaagaag gataacggac
     4381 cagtgatcaa aaagataaag tactatggca acaaacttaa tgcgcatttg gacataactg
     4441 acgattaccc caattctcga aacaaggttg tgaagctctc cctgaagcct tatagatttg
     4501 acgtgtacct ggataatggg gtttataaat tcgtcaccgt gaaaaatctg gacgtgatca
     4561 aaaaggagaa ctattatgaa gtaaactcaa agtgctatga ggaggcgaag aagctgaaga
     4621 agatctccaa tcaggccgag ttcatcgctt ccttctataa gaacgatctc atcaagatca
     4681 atggagagct ttatcgcgtc attggtgtga acaatgactt gctgaacagg atcgaagtca
     4741 atatgataga cattacctac cgggagtatc tcgaaaacat gaatgataaa cggccgcctc
     4801 acatcatcaa gacaatcgca tctaaaactc agtcaataaa aaagtactct accgatatcc
     4861 tggggaatct ctatgaagtg aagtcaaaga agcacccaca aatcattaaa aaaggtggat
     4921 cccccaagaa gaagaggaaa gtctcgagcg actacaaaga ccatgacggt gattataaag
     4981 atcatgacat cgattacaag gatgacgatg acaagtaaag cggccgcact cctcaggttt
     5041 aaacggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     5101 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5161 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5221 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     5281 atcgcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccggg ccgctcgaat
     5341 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5401 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5461 aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     5521 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     5581 atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgct tagccctccc
     5641 acacataacc agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt
     5701 ccttcactat ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc
     5761 aggggctcaa gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag
     5821 ctgccgcagc agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat
     5881 gatatacatt gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc
     5941 tgtagtcttc agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg
     6001 attcttcttg agacaaaggc ttggccatgg tttagttcct caccttgtcg tattatacta
     6061 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga
     6121 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     6181 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     6241 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     6301 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6361 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     6421 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     6481 aattcaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
     6541 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
     6601 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
     6661 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
     6721 tccgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     6781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7081 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7141 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7441 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7501 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7561 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7621 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7681 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7861 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7921 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     8941 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9001 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9061 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9121 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9181 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9241 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9301 gcgggcgcta gggcgcggcg ggtggggggg tcacgcggcg cgtaaccacc acacccgccg
     9361 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     9421 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     9481 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     9541 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggc atatggttct
     9601 tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg
     9661 aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa
     9721 aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag
     9781 atcgatctcg aaggatctgg aggccaccat ggtgtcgata acttcgtata gcatacatta
     9841 tacgaagtta tcgataagct tgctc
//
