LOCUS       sequence-223681-        5919 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, 
            Cojocaru V, Prabhakar S, Jauch R
  TITLE     DNA-mediated dimerization on a compact sequence signature controls 
            enhancer engagement and regulation by FOXA1.
  JOURNAL   Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 
            10.1093/nar/gky259.
  PUBMED    29669022
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-06-20- 20"; volume: "46"; issue: 
            "11"; pages: "5470-5486"
COMMENT     Sequence Label: QSOX1-Tol2
FEATURES             Location/Qualifiers
     source          1..5919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(696..715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(697..715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(722..739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(722..738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(731..753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      879..1334
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(966..985)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1176..1197
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1412..1516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1517..1585,1586..2377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1735..1754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2548..3136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3037..3056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3290..3307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3424..3445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3460..3465,3466..3483,3484..3490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3498..3514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3503..3525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3522..3538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3522..3538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3556..3574
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3556..3573
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(4877..4879,4880..4882,4883..5596)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4877..4879,4880..4882,4883..5593)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4922..4943)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5183..5202)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5530..5551
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5719..5840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5756..5775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5810..5829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaaaaaaact atcagtaaaa caaaatactt aatgtactta cctcaatatg cttccttagg
       61 tttgatggcg aacttttgaa ggccgatatt tctttattaa gcgggagaca gaggacacat
      121 ttcatcttga atgaatcttt atttacacca cttaaagaaa agaattcgcg aagatacggc
      181 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
      241 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
      301 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
      361 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
      421 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
      481 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
      541 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
      601 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
      661 ttactcaagt actttacacc tctgggccca attcgcccta tagtgagtcg tattacaatt
      721 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      781 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      841 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
      901 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      961 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1021 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1081 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1141 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1201 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1261 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1321 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     1381 cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2041 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3421 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     3481 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3541 tgattacgcc aagctattta ggtgacacta tagaatactc aagctatgca tccaacgcgt
     3601 tgggagctct cccatatggt cgagcagagg tgtaaaaagt actcaaaaat tttactcaag
     3661 tgaaagtaca agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga
     3721 atcttacttg agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa
     3781 gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt taagctttta atctcaaaaa
     3841 acattaaatg aaatgcatac aaggttttat cctgctttag aactgtttgt atttaattat
     3901 caaactataa gacagacaat ctaatgccag tacacgctac tcaaagttgt aaaacctcag
     3961 atttaacttc agtagaagct gattctcaaa attgttagtg tcaagcctag ctcttttggg
     4021 gctgaaaagc aatcctgcag tcgacgtcgg ccatagcggc cgcggaaggt tggtctcgct
     4081 tcatctctgt gtcctgaggt ttcttgagaa aggttggttg tgggcccgca gagggctgtg
     4141 ctcatcccac cctgggctgt gtgagtactg cccatgctct ctgctgagga cacctgtatc
     4201 ttgaggcagg aacagagaca gagtgaaggt cacagggaga gagaagaatt ttgcagccat
     4261 atttggctgg cttcaggcat ttaatctgac tgaatagggt tcatttcttc agtgactagg
     4321 gcagtagatt ctctaggcac ctgctgcccc ggggcagagc atgtgtttct gagaacctgg
     4381 tttatcccct ttctattcca gagcagctcc ggttgtctca ctgtatgttt tggaaactgg
     4441 ttgtatccaa tttagacctt cattgctatt cactggctaa tcaatcagca aatatttgtt
     4501 gagtgataag cattgtttgc attgcagttg gggaaatgag ctactcatag aaggaaaatt
     4561 ggcaactgtt acaaagctgt gtttgaaaaa ggccaaagga gtggttcagc catttcttat
     4621 gggaacccag gggaggggaa agtcacagag ggctaatctg atccccagag gccttcatgc
     4681 cagtagttat ttattacacg cttgccttat gccagcactt tgctaagggc cttcacaggc
     4741 cattattatg acaatcctta cccgggaaaa tcgcggatcc cccagtgacg taggaagtcc
     4801 atccattcac agcgcttcta taaaggcgcc agctgaggcg cctactactc caaccgcgac
     4861 tgcagcgagc aactccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4921 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4981 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5041 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5101 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5161 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5221 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5281 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5341 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5401 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5461 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5521 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5581 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     5641 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5701 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5821 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatcctacta cacatataaa
     5881 aggcgcgcca tcaacgtcaa aacatgcaca cccaaaaaa
//
