LOCUS       sequence-223814-        9199 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Produces equimolar expression of yeast codon-optimized tdTomato and 
            mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW ytdtomato-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..9199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2285..2338
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2354..2363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2360..2362,2363..2365,2366..3079)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2405..2426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2666..2685)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3013..3034
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3089..3113)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3175..3194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3176..3194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3342..3529
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3397..3421)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3541..3558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3541..3557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3550..3572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3993..5339
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5450..6253)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6254..6469)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6494..6512)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6580..6684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6685..6753,6754..7545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6903..6922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7716..8304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8205..8224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8458..8475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8592..8613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8628..8633,8634..8651,8652..8658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8666..8682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8671..8693
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8690..8706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8690..8706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8736..9191)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8873..8894)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9085..9104
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc actagtaagc
      841 ttttaattaa atggttagta aaggtgagga agtgattaag gaattcatga ggtttaaagt
      901 tagaatggaa ggttctatga atggtcatga atttgaaatt gaaggtgaag gtgaaggtag
      961 accatatgaa ggtacacaaa ctgctaaatt gaaagttaca aaaggtggtc cattaccatt
     1021 tgcatgggat attttatctc cacagtttat gtatggtagt aaagcatatg ttaaacatcc
     1081 agctgatatt ccagattata agaaattaag ttttcctgaa ggttttaaat gggaaagagt
     1141 aatgaatttt gaagatggtg gtttagtaac agttactcaa gattcttctt tacaagatgg
     1201 tacattaata tataaagtta aaatgagagg tactaatttt ccacctgatg gtccagttat
     1261 gcagaagaaa acaatgggtt gggaagcctc aactgaaaga ttgtacccta gagatggagt
     1321 tttgaaaggt gaaattcatc aagctcttaa gttaaaagat ggaggtcact atttggttga
     1381 gtttaaaact atatacatgg ctaaaaaacc tgttcaattg ccaggttatt attatgttga
     1441 tactaaatta gatattactt cacataatga agattatact attgttgaac aatatgaaag
     1501 atctgaaggt agacatcatt tgtttttggg tcatggtact ggtagtactg gttctggttc
     1561 ttcaggtact gcttctagtg aagataataa tatggctgta atcaaagagt tcatgaggtt
     1621 taaagttaga atggaaggtt ctatgaatgg tcatgagttt gaaatcgaag gtgaaggaga
     1681 aggtagacca tatgaaggta ctcaaactgc taaattgaaa gttactaaag gtggtccatt
     1741 gccatttgct tgggatatat tgtcacctca gtttatgtat ggttctaaag catatgttaa
     1801 acatccagct gatattcctg attataaaaa attatcattt cctgaaggtt ttaaatggga
     1861 aagagttatg aattttgaag atggtggttt agttactgta actcaagatt cttcattgca
     1921 agatggtaca ttaatatata aagttaaaat gagaggtact aattttccac ctgatggtcc
     1981 agttatgcag aaaaagacta tgggttggga agcatctact gaaagattat atcctagaga
     2041 tggtgtttta aaaggtgaaa ttcaccaagc cttaaaattg aaagatggtg gtcattattt
     2101 ggttgaattc aaaacaatct acatggctaa aaaaccagtt cagctgcccg gatactatta
     2161 tgtcgatact aaactggata ttacttcaca caatgaagac tacacaattg ttgaacagta
     2221 tgaaagatca gagggtagac atcacttgtt tttatacggt atggatgaat tatataaatc
     2281 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg
     2341 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2401 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2461 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2521 gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg
     2581 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2641 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2701 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2761 cggcaacatc ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac
     2821 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     2881 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2941 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     3001 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3061 ggacgagctg tacaagtaaa gcggccgcac ccagctttct tgtacaaagt ggtgtagtta
     3121 attcatgatg gccgctgcag gtcgacctcg agggggggcc cggtacccaa ttcgccctat
     3181 agtgagtcgt attacgcgcg gatccagctt tggacttctt cgccagaggt ttggtcaagt
     3241 ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg
     3301 gtcaaatcgt tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta
     3361 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     3421 cttaggtttt aaaacganaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     3481 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccaattc
     3541 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3601 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3661 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     3721 tacgcatctg tgcggtattt cacaccgcat aatcggatcg tacttgttac ccatcattga
     3781 attttgaaca tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat
     3841 aataatatat agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa
     3901 ctattgcatc tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt
     3961 tccatcttgc acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag
     4021 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     4081 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     4141 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     4201 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     4261 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     4321 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     4381 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     4441 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     4501 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     4561 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     4621 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     4681 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
     4741 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
     4801 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
     4861 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
     4921 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga
     4981 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
     5041 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
     5101 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
     5161 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
     5221 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
     5281 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg
     5341 atccgatgat aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag
     5401 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     5461 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     5521 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     5581 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     5641 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     5701 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     5761 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     5821 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     5881 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     5941 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     6001 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     6061 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     6121 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     6181 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     6241 tagctttcga catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta
     6301 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     6361 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     6421 aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctaattct
     6481 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6541 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6901 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6961 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7021 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7081 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7141 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7201 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7261 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7321 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7381 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7441 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7501 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7561 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7621 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7681 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7741 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7801 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7861 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7921 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7981 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8041 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8101 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8161 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8221 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8281 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8341 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8401 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8461 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8521 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8581 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8641 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8701 tatgaccatg attacgccaa gcttaccgca tcaggaaatt gtaagcgtta atattttgtt
     8761 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8821 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8881 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8941 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     9001 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     9061 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     9121 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9181 acagggcgcg tccattcgc
//
