LOCUS       sequence-224056-       15994 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Self-inactivating DG-Rabies (SiR) expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15994)
  AUTHORS   Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
  TITLE     Life-Long Genetic and Functional Access to Neural Circuits Using 
            Self-Inactivating Rabies Virus.
  JOURNAL   Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 
            10.1016/j.cell.2017.06.014.
  PUBMED    28689641
REFERENCE   2  (bases 1 to 15994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014."
COMMENT     Sequence Label: pSAD-F3-NPEST-mCherry
FEATURES             Location/Qualifiers
     source          1..15994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2456..2467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7565..7582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7571..7795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7841..8269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7928..7947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8138..8159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8278..8298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8283..8612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8463..8598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8525..8544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8660..8707
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8726..9100
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9230..9351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9267..9286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9321..9340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9400..9416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9400..9416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9413..9435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9424..9440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9448..9454,9455..9472,9473..9478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9493..9514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9631..9648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9802..10390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9882..9901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10561..11352,11353..11421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11184..11203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11422..11526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11601..11620)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11792..12171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12172..12375
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12326..12346
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12420..12439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12420..12438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             13934..13954
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      join(15989..15994,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg actcgagggc
      721 gcgcctaccc gcggtagctt ttcagtcgag aaaaaaacat tagatcagaa gaacaactgg
      781 caacacttct caacctgaga cttacttcaa gatgctcgat cctggagagg tctatgatga
      841 ccctattgac ccaatcgagt tagaggctga acccagagga acccccattg tccccaacat
      901 cttgaggaac tctgactaca atctcaactc tcctttgata gaagatcctg ctagactaat
      961 gttagaatgg ttaaaaacag ggaatagacc ttatcggatg actctaacag acaattgctc
     1021 caggtctttc agagttttga aagattattt caagaaggta gatttgggtt ctctcaaggt
     1081 gggcggaatg gctgcacagt caatgatttc tctctggtta tatggtgccc actctgaatc
     1141 caacaggagc cggagatgta taacagactt ggcccatttc tattccaagt cgtcccccat
     1201 agagaagctg ttgaatctca cgctaggaaa tagagggctg agaatccccc cagagggagt
     1261 gttaagttgc cttgagaggg ttgattatga taatgcattt ggaaggtatc ttgccaacac
     1321 gtattcctct tacttgttct tccatgtaat caccttatac atgaacgccc tagactggga
     1381 tgaagaaaag accatcctag cattatggaa agatttaacc tcagtggaca tcgggaagga
     1441 cttggtaaag ttcaaagacc aaatatgggg actgctgatc gtgacaaagg actttgttta
     1501 ctcccaaagt tccaattgtc tttttgacag aaactacaca cttatgctaa aagatctttt
     1561 cttgtctcgc ttcaactcct taatggtctt gctctctccc ccagagcccc gatactcaga
     1621 tgacttgata tctcaactat gccagctgta cattgctggg gatcaagtct tgtctatgtg
     1681 tggaaactcc ggctatgaag tcatcaaaat attggagcca tatgtcgtga atagtttagt
     1741 ccagagagca gaaaagttta ggcctctcat tcattccttg ggagactttc ctgtatttat
     1801 aaaagacaag gtaagtcaac ttgaagagac gttcggtccc tgtgcaagaa ggttctttag
     1861 ggctctggat caattcgaca acatacatga cttggttttt gtgtttggct gttacaggca
     1921 ttgggggcac ccatatatag attatcgaaa gggtctgtca aaactatatg atcaggttca
     1981 ccttaaaaaa atgatagata agtcctacca ggagtgctta gcaagcgacc tagccaggag
     2041 gatccttaga tggggttttg ataagtactc caagtggtat ctggattcaa gattcctagc
     2101 ccgagaccac cccttgactc cttatatcaa aacccaaaca tggccaccca aacatattgt
     2161 agacttggtg ggggatacat ggcacaagct cccgatcacg cagatctttg agattcctga
     2221 atcaatggat ccgtcagaaa tattggatga caaatcacat tctttcacca gaacgagact
     2281 agcttcttgg ctgtcagaaa accgaggggg gcctgttcct agcgaaaaag ttattatcac
     2341 ggccctgtct aagccgcctg tcaatccccg agagtttctg aggtctatag acctcggagg
     2401 attgccagat gaagacttga taattggcct caagccaaag gaacgggaat tgaagattga
     2461 aggtcgattc tttgctctaa tgtcatggaa tctaagattg tattttgtca tcactgaaaa
     2521 actcttggcc aactacatct tgccactttt tgacgcgctg actatgacag acaacctgaa
     2581 caaggtgttt aaaaagctga tcgacagggt caccgggcaa gggcttttgg actattcaag
     2641 ggtcacatat gcatttcacc tggactatga aaagtggaac aaccatcaaa gattagagtc
     2701 aacagaggat gtattttctg tcctagatca agtgtttgga ttgaagagag tgttttctag
     2761 aacacacgag ttttttcaaa aggcctggat ctattattca gacagatcag acctcatcgg
     2821 gttacgggag gatcaaatat actgcttaga tgcgtccaac ggcccaacct gttggaatgg
     2881 ccaggatggc gggctagaag gcttacggca gaagggctgg agtctagtca gcttattgat
     2941 gatagataga gaatctcaaa tcaggaacac aagaaccaaa atactagctc aaggagacaa
     3001 ccaggtttta tgtccgacat acatgttgtc gccagggcta tctcaagagg ggctcctcta
     3061 tgaattggag agaatatcaa ggaatgcact ttcgatatac agagccgtcg aggaaggggc
     3121 atctaagcta gggctgatca tcaagaaaga agagaccatg tgtagttatg acttcctcat
     3181 ctatggaaaa acccctttgt ttagaggtaa catattggtg cctgagtcca aaagatgggc
     3241 cagagtctct tgcgtctcta atgaccaaat agtcaacctc gccaatataa tgtcgacagt
     3301 gtccaccaat gcgctaacag tggcacaaca ctctcaatct ttgatcaaac cgatgaggga
     3361 ttttctgctc atgtcagtac aggcagtctt tcactacctg ctatttagcc caatcttaaa
     3421 gggaagagtt tacaagattc tgagcgctga aggggagagc tttctcctag ccatgtcaag
     3481 gataatctat ctagatcctt ctttgggagg gatatctgga atgtccctcg gaagattcca
     3541 tatacgacag ttctcagacc ctgtctctga agggttatcc ttctggagag agatctggtt
     3601 aagctcccaa gagtcctgga ttcacgcgtt gtgtcaagag gctggaaacc cagatcttgg
     3661 agagagaaca ctcgagagct tcactcgcct tctagaagat ccgaccacct taaatatcag
     3721 aggaggggcc agtcctacca ttctactcaa ggatgcaatc agaaaggctt tatatgacga
     3781 ggtggacaag gtggaaaatt cagagtttcg agaggcaatc ctgttgtcca agacccatag
     3841 agataatttt atactcttct taatatctgt tgagcctctg tttcctcgat ttctcagtga
     3901 gctattcagt tcgtcttttt tgggaatccc cgagtcaatc attggattga tacaaaactc
     3961 ccgaacgata agaaggcagt ttagaaagag tctctcaaaa actttagaag aatccttcta
     4021 caactcagag atccacggga ttagtcggat gacccagaca cctcagaggg ttgggggggt
     4081 gtggccttgc tcttcagaga gggcagatct acttagggag atctcttggg gaagaaaagt
     4141 ggtaggcacg acagttcctc acccttctga gatgttggga ttacttccca agtcctctat
     4201 ttcttgcact tgtggagcaa caggaggagg caatcctaga gtttctgtat cagtactccc
     4261 gtcctttgat cagtcatttt tttcacgagg ccccctaaag ggatacttgg gctcgtccac
     4321 ctctatgtcg acccagctat tccatgcatg ggaaaaagtc actaatgttc atgtggtgaa
     4381 gagagctcta tcgttaaaag aatctataaa ctggttcatt actagagatt ccaacttggc
     4441 tcaagctcta attaggaaca ttatgtctct gacaggccct gatttccctc tagaggaggc
     4501 ccctgtcttc aaaaggacgg ggtcagcctt gcataggttc aagtctgcca gatacagcga
     4561 aggagggtat tcttctgtct gcccgaacct cctctctcat atttctgtta gtacagacac
     4621 catgtctgat ttgacccaag acgggaagaa ctacgatttc atgttccagc cattgatgct
     4681 ttatgcacag acatggacat cagagctggt acagagagac acaaggctaa gagactctac
     4741 gtttcattgg cacctccgat gcaacaggtg tgtgagaccc attgacgacg tgaccctgga
     4801 gacctctcag atcttcgagt ttccggatgt gtcgaaaaga atatccagaa tggtttctgg
     4861 ggctgtgcct cacttccaga ggcttcccga tatccgtctg agaccaggag attttgaatc
     4921 tctaagcggt agagaaaagt ctcaccatat cggatcagct caggggctct tatactcaat
     4981 cttagtggca attcacgact caggatacaa tgatggaacc atcttccctg tcaacatata
     5041 cggcaaggtt tcccctagag actatttgag agggctcgca aggggagtat tgataggatc
     5101 ctcgatttgc ttcttgacaa gaatgacaaa tatcaatatt aatagacctc ttgaattggt
     5161 ctcaggggta atctcatata ttctcctgag gctagataac catccctcct tgtacataat
     5221 gctcagagaa ccgtctctta gaggagagat attttctatc cctcagaaaa tccccgccgc
     5281 ttatccaacc actatgaaag aaggcaacag atcaatcttg tgttatctcc aacatgtgct
     5341 acgctatgag cgagagataa tcacggcgtc tccagagaat gactggctat ggatcttttc
     5401 agactttaga agtgccaaaa tgacgtacct atccctcatt acttaccagt ctcatcttct
     5461 actccagagg gttgagagaa acctatctaa gagtatgaga gataacctgc gacaattgag
     5521 ttctttgatg aggcaggtgc tgggcgggca cggagaagat accttagagt cagacgacaa
     5581 cattcaacga ctgctaaaag actctttacg aaggacaaga tgggtggatc aagaggtgcg
     5641 ccatgcagct agaaccatga ctggagatta cagccccaac aagaaggtgt cccgtaaggt
     5701 aggatgttca gaatgggtct gctctgctca acaggttgca gtctctacct cagcaaaccc
     5761 ggcccctgtc tcggagcttg acataagggc cctctctaag aggttccaga accctttgat
     5821 ctcgggcttg agagtggttc agtgggcaac cggtgctcat tataagctta agcctattct
     5881 agatgatctc aatgttttcc catctctctg ccttgtagtt ggggacgggt caggggggat
     5941 atcaagggca gtcctcaaca tgtttccaga tgccaagctt gtgttcaaca gtcttttaga
     6001 ggtgaatgac ctgatggctt ccggaacaca tccactgcct ccttcagcaa tcatgagggg
     6061 aggaaatgat atcgtctcca gagtgataga tcttgactca atctgggaaa aaccgtccga
     6121 cttgagaaac ttggcaacct ggaaatactt ccagtcagtc caaaagcagg tcaacatgtc
     6181 ctatgacctc attatttgcg atgcagaagt tactgacatt gcatctatca accggatcac
     6241 cctgttaatg tccgattttg cattgtctat agatggacca ctctatttgg tcttcaaaac
     6301 ttatgggact atgctagtaa atccaaacta caaggctatt caacacctgt caagagcgtt
     6361 cccctcggtc acagggttta tcacccaagt aacttcgtct ttttcatctg agctctacct
     6421 ccgattctcc aaacgaggga agtttttcag agatgctgag tacttgacct cttccaccct
     6481 tcgagaaatg agccttgtgt tattcaattg tagcagcccc aagagtgaga tgcagagagc
     6541 tcgttccttg aactatcagg atcttgtgag aggatttcct gaagaaatca tatcaaatcc
     6601 ttacaatgag atgatcataa ctctgattga cagtgatgta gaatcttttc tagtccacaa
     6661 gatggttgat gatcttgagt tacagagggg aactctgtct aaagtggcta tcattatagc
     6721 catcatgata gttttctcca acagagtctt caacgtttcc aaacccctaa ctgacccctc
     6781 gttctatcca ccgtctgatc ccaaaatcct gaggcacttc aacatatgtt gcagtactat
     6841 gatgtatcta tctactgctt taggtgacgt ccctagcttc gcaagacttc acgacctgta
     6901 taacagacct ataacttatt acttcagaaa gcaagtcatt cgagggaacg tttatctatc
     6961 ttggagttgg tccaacgaca cctcagtgtt caaaagggta gcctgtaatt ctagcctgag
     7021 tctgtcatct cactggatca ggttgattta caagatagtg aagactacca gactcgttgg
     7081 cagcatcaag gatctatcca gagaagtgga aagacacctt cataggtaca acaggtggat
     7141 caccctagag gatatcagat ctagatcatc cctactagac tacagttgcc tgtgaaccgg
     7201 atactcctgg aagcctgccc atgctaagac tcttgtgtga tgtatcttga aaaaaacaag
     7261 atcctaaatc tgaacctttg gttgtttgat tgtttttctc atttttgttg tttatttgtt
     7321 aagcgtgggt cggcatggca tctccacctc ctcgcggtcc gacctgggca tccgaaggag
     7381 gacgcacgtc cactcggatg gctaagggag aagggcgaat tccagcacac tggcggccgt
     7441 tactagtgga tccgagctcg gtaccgagct cggatccact agtccagtgt ggtggaattc
     7501 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     7561 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7621 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7681 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7741 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7801 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     8281 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8341 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8401 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8521 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8581 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8641 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8701 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8761 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8821 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8881 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8941 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     9001 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     9061 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     9121 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9181 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9361 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11521 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    11581 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    11641 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    11701 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    11761 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
    11821 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    11881 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    11941 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    12001 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    12061 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    12121 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    12181 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    12241 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    12301 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    12361 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
    12421 aatacgactc actataggga gacccaagct ggctagatta agcgtctgat gagtccgtga
    12481 ggacgaaacc cggagtcccg ggtcacgctt aacaaccaga tcaaagaaaa aacagacatt
    12541 gtcaattgca aagcaaaaat gtaacacccc tacaatggat gccgacaaga ttgtattcaa
    12601 agtcaataat caggtggtct ctttgaagcc tgagattatc gtggatcaat atgagtacaa
    12661 gtaccctgcc atcaaagatt tgaaaaagcc ctgtataacc ctaggaaagg ctcccgattt
    12721 aaataaagca tacaagtcag ttttgtcagg catgagcgcc gccaaactta atcctgacga
    12781 tgtatgttcc tatttggcag cggcaatgca gttttttgag gggacatgtc cggaagactg
    12841 gaccagctat ggaattgtga ttgcacgaaa aggagataag atcaccccag gttctctggt
    12901 ggagataaaa cgtactgatg tagaagggaa ttgggctctg acaggaggca tggaactgac
    12961 aagagacccc actgtccctg agcatgcgtc cttagtcggt cttctcttga gtctgtatag
    13021 gttgagcaaa atatccgggc aaaacactgg taactataag acaaacattg cagacaggat
    13081 agagcagatt tttgagacag ccccttttgt taaaatcgtg gaacaccata ctctaatgac
    13141 aactcacaaa atgtgtgcta attggagtac tataccaaac ttcagatttt tggccggaac
    13201 ctatgacatg tttttctccc ggattgagca tctatattca gcaatcagag tgggcacagt
    13261 tgtcactgct tatgaagact gttcaggact ggtatcattt actgggttca taaaacaaat
    13321 caatctcacc gctagagagg caatactata tttcttccac aagaactttg aggaagagat
    13381 aagaagaatg tttgagccag ggcaggagac agctgttcct cactcttatt tcatccactt
    13441 ccgttcacta ggcttgagtg ggaaatctcc ttattcatca aatgctgttg gtcacgtgtt
    13501 caatctcatt cactttgtag gatgctatat gggtcaagtc agatccctaa atgcaacggt
    13561 tattgctgca tgtgctcctc atgaaatgtc tgttctaggg ggctatctgg gagaggaatt
    13621 cttcgggaaa gggacatttg aaagaagatt cttcagagat gagaaagaac ttcaagaata
    13681 cgaggcggct gaactgacaa agactgacgt agcactggca gatgatggaa ctgtcaactc
    13741 tgacgacgag gactactttt caggtgaaac cagaagtccg gaggctgttt atactcgaat
    13801 catgatgaat ggaggtcgac taaagagatc tcacatacgg agatatgtct cagtcagttc
    13861 caatcatcaa gcccgtccaa actcattcgc cgagtttcta aacaagacat attcgagtga
    13921 ctcaggttcc ggagagaacc tctacttcca atcgggatcc ggtagccatg gcttcccgcc
    13981 ggaggtggag gagcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat
    14041 ggaccgtcac cctgcagcct gtgcttctgc taggatcaat gtgtaagaag ttgaataaca
    14101 aaatgccgga aatctacgga ttgtgtatat ccatcatgaa aaaaactaac acccctcctt
    14161 tcgaaccatc ccaaacatga gcaagatctt tgtcaatcct agtgctatta gagccggtct
    14221 ggccgatctt gagatggctg aagaaactgt tgatctgatc aatagaaata tcgaagacaa
    14281 tcaggctcat ctccaagggg aacccataga ggtggacaat ctccctgagg atatggggcg
    14341 acttcacctg gatgatggaa aatcgcccaa ccatggtgag atagccaagg tgggagaagg
    14401 caagtatcga gaggactttc agatggatga aggagaggat cctagcttcc tgttccagtc
    14461 atacctggaa aatgttggag tccaaatagt cagacaaatg aggtcaggag agagatttct
    14521 caagatatgg tcacagaccg tagaagagat tatatcctat gtcgcggtca actttcccaa
    14581 ccctccagga aagtcttcag aggataaatc aacccagact actggccgag agctcaagaa
    14641 ggagacaaca cccactcctt ctcagagaga aagccaatca tcgaaagcca ggatggcggc
    14701 tcaaattgct tctggccctc cagcccttga atggtcggct accaatgaag aggatgatct
    14761 atcagtggag gctgagatcg ctcaccagat tgcagaaagt ttctccaaaa aatataagtt
    14821 tccctctcga tcctcaggga tactcttgta taattttgag caattgaaaa tgaaccttga
    14881 tgatatagtt aaagaggcaa aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc
    14941 caaactcccc ctaagatgtg tactgggatg ggtcgctttg gccaactcta agaaattcca
    15001 gttgttagtc gaatccgaca agctgagtaa aatcatgcaa gatgacttga atcgctatac
    15061 atcttgctaa ccgaacctct cccctcagtc cctctagaca ataaaatccg agatgtccca
    15121 aagtcaacat gaaaaaaaca ggcaacacca ctgataaatc gatgaacctc ctacgtaaga
    15181 tagtgaaaaa ccgcagggac gaggacactc aaaaatcctc tcccgcgtca gcccctctgg
    15241 atgacgatga cttgtggctt ccaccccctg aatacgtccc gctgaaagaa cttacaggca
    15301 agaagaacat gaggaacttt tgtatcaacg gaagggttaa agtgtgtagc ccgaatggtt
    15361 actcgttcag gatcctgcgg cacattctga aatcattcga cgagatatat tctgggaatc
    15421 ataggatgat cgggttagtc aaagtggtta ttggactggc tttgtcagga tctccagtcc
    15481 ctgagggcct gaactgggta tacaaattga ggagaacctt tatcttccag tgggctgatt
    15541 ccaggggccc tcttgaaggg gaggagttgg aatactctca ggagatcact tgggatgatg
    15601 atactgagtt cgtcggattg caaataagag tgattgcaaa acagtgtcat atccagggca
    15661 gagtctggtg tatcaacatg aacccgagag catgtcaact atggtctgac atgtctcttc
    15721 agacacaaag gtccgaagag gacaaagatt cctctctgct tctagaataa cgtacgtcag
    15781 attatatccc gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac
    15841 tccaaccctt gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt
    15901 tcatcaaagt caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat
    15961 taacatccct caaaggacct gcaggtacgc cacc
//
