LOCUS       sequence-224068-        3304 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Multisite gateway vector for 3' tagging with constitutively active 
            rab5c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab5c-CA
FEATURES             Location/Qualifiers
     source          1..3304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..25)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(690..785)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(822..841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(823..841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(846..862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(846..862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(964..983)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             975..1784
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1042..1061)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1931..2519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2420..2439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2673..2690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2849..2876)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2968..3054)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3103..3125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3117..3134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3118..3134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gttcaacttt cttgtacaaa gtggtggcgg ggcgaggtgg accagcgcgg accaacggtg
       61 ccgcccccgt cgggaacaaa atctgccagt ttaagcttgt gttgctggga gagtctgcag
      121 taggcaagtc cagcctggtg ctgcgcttcg tcaaaggcca gtttcacgag taccaggaga
      181 gcaccattgg agctgctttc ctcacacaga cactctgctt ggacgatacg acagtaaagt
      241 ttgagatttg ggacacggcc ggactggagc ggtatcacag tttggcccct atgtattaca
      301 gaggagccca ggcagccatc gtcgtctacg acattactaa cacagacaca ttcactagag
      361 caaagaactg ggtgaaggag ctgcagcgcc aggccagtcc aaatatcgtt attgctctgg
      421 caggcaacaa agcagatctg gccaacaaga gagccgttga ctttcaggag gcccaggcat
      481 acgcagatga caacagccta ctcttcatgg agacgtctgc aaagaccgcc atgaacgtca
      541 atgagatctt catggctatc gccaagaagc ttccgaaaag cgagccgcag ggtggagcgg
      601 gcagcggggg acgggcccga ggaggggtgg acttacagga gacggcgcct cagggaagaa
      661 gcggccagtg ctgtggaggc ggaaactaac aactttatta tacaaagttg gcattataaa
      721 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      781 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt
      841 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      901 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
      961 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1021 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1081 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1141 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1201 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1261 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1321 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1381 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     1441 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1501 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1561 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1621 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1681 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1741 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1801 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     1861 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     1921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2041 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2701 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2761 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     2821 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     2881 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     2941 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     3001 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     3061 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     3121 aaacgacggc cagtcttaag ctcgggccct gcagctctag agctcgaatt ctacaggtca
     3181 ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt acagtattat
     3241 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     3301 tctc
//
