LOCUS       sequence-224099-        8771 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Melanocyte-specific expression of the LexPR-2A-Cerulean fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Kenyon A, Gavriouchkina D, Zorman J, Chong-Morrison V, Napolitani G,
            Cerundolo V, Sauka-Spengler T
  TITLE     Generation of a double binary transgenic zebrafish model to study 
            myeloid gene regulation in response to oncogene activation in 
            melanocytes.
  JOURNAL   Dis Model Mech. 2018 Apr 6;11(4). pii: dmm.030056. doi: 
            10.1242/dmm.030056.
  PUBMED    29666124
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2018-04-6"; volume: "11(4). pii: dmm.030056. doi"; 
            pages: " 10.1242/dmm.030056"
COMMENT     Sequence Label: 
            pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
FEATURES             Location/Qualifiers
     source          1..8771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             52..846
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             52..846
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    853..901
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(926..973)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(1249..1267)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1250..1267)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1285..1301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1285..1301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1298..1320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1309..1325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1333..1339,1340..1357,1358..1363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1378..1399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1516..1533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1687..2275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1767..1786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2446..3237,3238..3306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3069..3088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3324..4118)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(4462..4890)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4572..4593)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4784..4803
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5016..5038
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5030..5047
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5031..5047
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5054..5073
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5054..5072
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5338..5589
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             6880..7251
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             7267..7320
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7333..7335,7336..8049)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7375..7396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7636..7655)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7983..8004
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8101..8222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8112..8131)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8166..8185
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8346..8393)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        8487..8591
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8616..8636)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 taaccatagt ccgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
       61 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      121 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      181 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
      241 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      301 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      361 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      421 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      481 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      541 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
      601 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      661 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      721 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      781 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      841 ttctgagggg atcggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
      901 cggatccgag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
      961 gaatagtaac ttctccatgg tagcctccaa aaaagcctcc tcactacttc tggaaaagct
     1021 caatcagtga gatgggattt tgtgtgttaa ccttgaagaa aaatattctt taacacatat
     1081 taagacgtaa gcagattaga atattcattt aatcttctgt atgtttgcat gcttgcatat
     1141 ttatagactg agcggttata aaatacgtaa agggcgaatt ccagcacact ggcggccgtt
     1201 actagtggat ccgagctcgg taccaagctt gatgcatagc ttgagtattc tatagtgtca
     1261 cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1321 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1381 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1441 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1501 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1561 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1621 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2101 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2341 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2401 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2461 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2521 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2581 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2641 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2701 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2761 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2821 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2881 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2941 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3001 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3061 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3121 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3181 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3241 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3301 actcatactc ttcctttttc aattcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3541 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     3601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3661 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3781 actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat
     3841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     3961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     4141 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     4201 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     4261 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     4321 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     4381 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     4441 ttgcgccctg aattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     4501 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     4561 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4621 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4681 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4741 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4801 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4861 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     4921 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4981 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5041 ggccagtgaa ttgtaatacg actcactata gggcgaattg ggccctctag atgcatgctc
     5101 gagcggccgc cagtgtgatg gatatctgca gaattcgccc tttacgtaaa aaaaacaata
     5161 tgagaagagc aatcccctcc caccccctta aatacatttt gtgtcttttc tttggcattg
     5221 acatcttcag ctggccaaga cgactggtca gttcttgcac atcacctaaa ttcacaaagg
     5281 gaatactttt ttgaagctaa cacatagttg aacatggcac ccaagaagaa gaggaagatg
     5341 aaagcgttaa cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag
     5401 acaggaatgc cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac
     5461 gcggctgaag aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc
     5521 gcatcacgcg ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggttggtcgt
     5581 gtggctgccg gtgaaccgtc gagcgccgga aaaaagttca ataaagtcag agttgtgaga
     5641 gcactggatg ctgttgctct cccacagcca gtgggcgttc caaatgaaag ccaagcccta
     5701 agccagagat tcactttttc acctggtcaa gacatacagt tgattccacc actgatcaac
     5761 ctgttaatga gcattgaacc agatgtgatc tatgcaggac atgacaacac aaaacctgac
     5821 acctccagtt ctttgctgac aagtcttaat caactaggcg agaggcaact tctttcagta
     5881 gtcaagtggt ctaaatcatt gccaggtttt cgaaacttac atattgatga ccagataact
     5941 ctcattcagt attcttggat gagcttaatg gtgtttggtc taggatggag atcctacaaa
     6001 cacgtcagtg ggcagatgct gtattttgca cctgatctaa tactaaatga acagcggatg
     6061 aaagaatcat cattctattc attatgcctt accatgtggc agatcccaca ggagtttgtc
     6121 aagcttcaag ttagccaaga agagttcctc tgtatgaaag tattgttact tcttaataca
     6181 attcctttgg aagggctacg aagtcaaacc cagtttgagg agatgaggtc aagctacatt
     6241 agagagctca tcaaggcaat tggtttgagg caaaaaggag ttgtgtcgag ctcacagcgt
     6301 ttctatcaac ttacaaaact tcttgataac ttgcatgatc ttgtcaaaca acttcatctg
     6361 tactgcttga atacatttat ccagtcccgg gcactgagtg ttgaatttcc agaaatgatg
     6421 tctgaagtta ttgctgggtc gacgcccatg gaattccagt acctgccaga tacagacgat
     6481 cgtcaccgga ttgaggagaa acgtaaaagg acatatgaga ccttcaagag catcatgaag
     6541 aagagtcctt tcagcggacc caccgacccc cggcctccac ctcgacgcat tgctgtgcct
     6601 tcccgctcct ccgcttctgt ccccaagcca gcaccccagc cctatccctt tacgtcatcc
     6661 ctgagcacca tcaactatga tgagtttccc accatggtgt ttccttctgg acagatcagc
     6721 caggcctcgg ccttggcccc ggcccctccc caagtcctgc cccaggctcc agcccctgcc
     6781 cctgctccag ccatggtatc agctctggcc caggccccag cccctgtccc agtcctagcc
     6841 cctggccctc ctcaagctgt ggccccacct gcccccaagc ccacccaggc tggggaagga
     6901 acgctgtcag aggccctgct gcagctgcag tttgatgatg aagacctggg ggccttgctt
     6961 ggcaacagca cagacccagc tgtgttcaca gacctggcat ccgtcgacaa ctccgagttt
     7021 cagcagctgc tgaaccaggg catacctgtg gcccctcaca caactgagcc catgctgatg
     7081 gagtaccctg aggctataac tcgcctagtg acaggggccc agaggcctcc ggacccagct
     7141 cctgctccac tgggggcccc ggggctcccc aatggcctcc tttctggaga tgaagacttc
     7201 tcctccattg cggacatgga cttctcagcc ctgctgagtc agatcagctc cgatatcgga
     7261 tcaggagagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccgggccc
     7321 cggtcgcgat cggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     7381 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     7441 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     7501 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     7561 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     7621 accatcttct tcaaagacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     7681 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     7741 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     7801 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     7861 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     7921 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     7981 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8041 tacaagtaaa gcggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
     8101 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     8161 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     8221 ttaacaacaa caattgcatt cattttatgt ttcgggttca gggggaggtg tgggaggttt
     8281 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgacc
     8341 tgcttgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc
     8401 ccctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttcgt
     8461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8581 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     8641 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8701 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8761 caattagtca g
//
