LOCUS       sequence-224847-        8499 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 
            and 259) from the FRT site of FlpIn cells upon addition of 
            doxycyclin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Vleugel M, Tromer E, Omerzu M, Groenewold V, Nijenhuis W, Snel B, 
            Kops GJ
  TITLE     Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 
            promote accurate chromosome segregation.
  JOURNAL   J Cell Biol. 2013 Dec 23;203(6):943-55. doi: 10.1083/jcb.201307016.
  PUBMED    24344183
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-12-23- 23"; volume: "203"; 
            issue: "6"; pages: "943-55"
COMMENT     Sequence Label: pcDNA5-LAP-KNL1-M3
FEATURES             Location/Qualifiers
     source          1..8499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(410..412,413..415,416..1126)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(455..476)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(716..735)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1063..1084
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3837..3854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3843..4067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4200..4219)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4351..4398
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4406..5428
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4406..5428
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5558..5679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5595..5614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5649..5668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5728..5744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5728..5744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5741..5763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5752..5768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5776..5782,5783..5800,5801..5806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5821..5842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5959..5976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6130..6718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6210..6229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6889..7680,7681..7749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7512..7531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7750..7854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7929..7948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8120..8499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccgct agcgctaccg gtcgccacca tggtgagcaa
      421 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      481 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      541 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      601 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      661 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      721 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      781 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      841 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      901 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      961 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     1021 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1081 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gaggggatcg
     1141 gtggagctcc accggtggcg gccgcatgga tggggtgtct tcagaggcta atgaagaaaa
     1201 tgacaatata gagagacctg ttagaagacg gcattcttca atattgaaac ccccaaggag
     1261 tcctcttcag gacctcagag gtgggaatga aagagttcag gaatccaatg ctttgagaaa
     1321 taagaaaaac tctcgtcgag tcagctttgc agatactata aaggtattcc agacggagtc
     1381 tcatatgaaa atagtgagaa agtcagaaat ggaaggctgc agtctcgacg gtccttctga
     1441 actacaagaa cttggtaaaa ctaatttaga acacactact ggccagctaa caacaatgaa
     1501 cagacagata gctgtaaaag ttgaaaaatg tggtaaaagt cccatagaaa aaagtggagt
     1561 gcttaaatct aactgtatta tggatgtgtt agaggacgaa agtgtacaga aacctaaatt
     1621 tccaaaggaa aagcaaaatg tcaaaatttg gggaaggaaa agtgttggtg gaccaaaaat
     1681 tgataagact attgtatttt cagaagacga taagaatgat atggatatca ctaagagtta
     1741 tacaatagaa ataaaccata gacctttatt agagaaacgt gattgtcatt tggtgccatt
     1801 ggcaggaact tctgaaacta ttttatatac atgtgggcag gatgacatgg agatcactag
     1861 aagtcacaca actgccttag aatgtaaaac tgtctcacca gatgaaataa ctactaggcc
     1921 tatggacaaa actgtagtgt ttgtagataa tcatgttgaa ctagaaatga cagagtccca
     1981 tactgttttc attgactacc aagaaaagga aagaacagac agacctaact ttgaactatc
     2041 ccaaaggaaa agcctaggaa caccaacagt gctcgagaac agtgtattga taaaaaacct
     2101 gagcaggacc ccatctagtt gcagcagctc tctggattca atcaaggctg atgggacctc
     2161 tctggacttc agcacttacc gcagtagtca aatggaatca cagtttctca gagatactat
     2221 ttgtgaagag agcttgaggg agaaactcca agatgggaga ataacaataa gggagttctt
     2281 tatacttctc caggtccaca tcttgataca gaaaccccga cagagcaatc tcccaggcaa
     2341 ttttactgta aacacaccac ctactccaga agacctgatg ttaagtcaat atgtttaccg
     2401 acccaagata cagatttata gagaagattg tgaggctcgt cgccaaaaga ttgaagaatt
     2461 aaagctttct gcatcgaacc aagataagct gttggttgat ataaataaga acctgtggga
     2521 aaaaatgaga cactgctctg acaaagagct gaaagccttt ggaatttatc ttaacaaaat
     2581 aaagtcatgt tttaccaaga tgactaaagt cttcactcac caaggaaaag tggctctgta
     2641 tggcaagctg gtgcagtcag ctcagaatga gagggagaaa cttcaaataa agatagatga
     2701 gatggataaa atacttaaga agatcgataa ctgcctcact gagatggaaa cagaaactaa
     2761 gaatttggag gatgaagaga aaaacaatcc tgtggaagaa tgggattctg aaatgagagc
     2821 tgcagaaaaa gaattggaac agctgaaaac tgaagaagag gagcttcaaa gaaatctctt
     2881 agaactggag gtacaaaaag agcagaccct tgctcaaata gactttatgc aaaaacaaag
     2941 aaatagaact gaagagctac tggatcagtt gagcttgtct gagtgggatg tcgttgagtg
     3001 gagtgatgat caagctgtat tcacctttgt ttatgacacg atacaactca ccatcacctt
     3061 tgaagagtca gttgttggtt tccctttcct ggacaagcgt tataggaaga ttgttgatgt
     3121 caattttcaa tctctgttag atgaggatca agctcctcct tcctcccttt tagttcataa
     3181 gcttattttc cagtacgttg aagaaaagga atcctggaag aagacatgta caacccagca
     3241 tcagttaccc aagatgcttg aagaattctc actggtagtg caccattgca gactccttgg
     3301 agaggagatt gagtatttaa agagatgggg accaaattat aacctaatga acatagatat
     3361 taataataat gaattgagac ttttattctc tagctccgca gcatttgcaa agtttgaaat
     3421 aactttgttt ctctcagcct attatccatc tgtaccatta ccttccacca ttcagaatca
     3481 cgttgggaac actagccaag atgatattgc taccattcta tctaaagtgc cactggagaa
     3541 caactacctg aagaatgtag tcaagcaaat ttaccaagat ctgtttcagg actgccattt
     3601 ctaccactag gttaaccaaa tggccaaggg aaccactgaa gaaatgaaat atgttctggg
     3661 ccaacttgtt ggtctgaatt ctcctaactc cattttgaaa gctgctaaaa ctttatatga
     3721 acactatagt ggtggtgaaa gtcataattc ttcatcctcc aagacttttg aaaaaaaaag
     3781 gggaaaaaaa tgagtcgagc atgcatctag agggcccgtt taaacccgct gatcagcctc
     3841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4021 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4081 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4321 acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct
     4381 agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     4441 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     4501 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     4561 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     4621 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     4681 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     4741 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     4801 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     4861 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     4921 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     4981 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     5041 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     5101 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     5161 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     5221 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     5281 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     5341 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     5401 cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc
     5461 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5521 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5701 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7861 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     7921 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     7981 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     8041 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     8101 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     8161 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     8221 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     8281 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     8341 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     8401 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     8461 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
