LOCUS       sequence-225086-       14393 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Pan neuronal expression of soma-localized Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-elavl3-Voltron-ST
FEATURES             Location/Qualifiers
     source          1..14393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    11350..11484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11374..11393)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11428..11447
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(12156..12172)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12156..12172)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12209..12229)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12209..12227)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12248..12264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12248..12264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12261..12283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12272..12288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12296..12302,12303..12320,12321..12326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12341..12362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12479..12496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12650..13238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12730..12749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13409..14200,14201..14269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14032..14051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14270..14374)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctattcctaa agaccttggg tgaccaaaat cttattttaa
      781 taaataaaac tgtttattaa aacttttttg tttcaaagaa ccatatgtat agtgaaattt
      841 ataaaaatat caatttttaa aaagctggtg tactcattta tgttatgaac tctaaaacca
      901 tatactgact gcaagtgatg atgtatagag tgatgtttac gagtaaacat atttagttgt
      961 atacatccta ctgagcacat tttgatgtat gaaataacat tacaagcttt atccaaatta
     1021 agccatttta aaacactgcc aattgaaaat acaaatcctg gaaaaaatcg tctttagcgc
     1081 agtcatttga gccatcctaa tccgttacct cagaccataa taagaaggga taacactagc
     1141 tgtagcaatg gaacacatct gtttcacaca atcatatctc ctgcgccggt gctaagcaga
     1201 ttcagcgtga tcataacatg ctttccactc ataaatgtaa atttacaatt tgcacatgta
     1261 aaacagacac ttttgagata ttggataaaa aaacaagagt atattgctta gtttcatcca
     1321 ccagtcatcc ccacagcgtt tggaaggcca taaaaagtgt ctaaaatcaa tgatcattga
     1381 aagagcacaa gagagactct tacgctgtaa tgccactggg gacaaaagtg acagtctctt
     1441 aatgggctct tctggagggg ctcctgaaca ttaaaaatta tcagcgaaat taccgaaaga
     1501 gcttcaagca actggcatgc ttgatcctct gcgtcggggc ggtgaatagg tgcttcagat
     1561 gccctcttac ccacgggctg gattcagctg ccccgctacc agcggagacc ccctaatgag
     1621 cctctgcaat taagtttatt catgttaagt gtgaacgggg tgcgtgcgga actgtgggca
     1681 gctaacagac ctgggttctt tgtgccacaa gtgctgcctt tattcggctc acaaagcaga
     1741 aaacaacacc cgcacctata atggcgccct cggctgggtc taagaaacgt ggcgagttga
     1801 cagagcagag tgggcggggt taagacagac tgacagcggg acccatctcc atcctcttat
     1861 taacgcttaa cgagtgcctt cctcatgcaa tattcatcgc cactaatatc atccaagctc
     1921 tgagctgagc tggccactta tgtaaggcaa ttatgtaaaa tatcagacag ggcccacact
     1981 cagaatctga ctggggtaga gacgcgggac gagaaccgag agcaagaact gaaagtgaaa
     2041 gtgaccacta aagggaggag aggacagagg ggcaggatgt gtcaagatta ccagagaaca
     2101 cttggccaga aatgcgcaac cattggagct ctccggatta cccaaaggtt aacgagtttg
     2161 aacgcctctg cccactcgcc catctctgat ggtttcccaa gaactcctca agcaaaatat
     2221 atataattgt gtgtattatg cacagacacg agaaaatgct gtttttctga tctgcattac
     2281 agcacatttg cccgccaacg acaataccac ccactcggta cctcgctgac tcctgatgcc
     2341 tgatacctgc gcggtgactg tctacaatct gcataatcaa gagaagttgt gttgaagacg
     2401 agcgccacac aaccgtttcc acaaggtcac ccaaggccgg tgcagatgta ggtgaggtct
     2461 ccataaacag actgaaataa acacatcctc cgctgggaac aacaaccccc tcacgcctca
     2521 tgcatttcca taagcctaca tgcatctctt ccaacttatg gagactcgca cctaccaaca
     2581 tccgcacaac aaagatatac agagcgcgct ccctcaggtc aaggccctgt gggggtctgt
     2641 gcagaaatag gtcatttgtc acacatcaag tcctggggca ggagatgcat tatagatgag
     2701 accaaacagc ctgtctcggt gagctctacc cactccctga gactagaaat gggggaaggg
     2761 agcttgagat aacaaccgct gcaatcactg tgtcgatgtt taatatcagc accaaccggg
     2821 aacaataagg agatgggtgc attcatgttc acatcttacc agtcaagtat catcgaaccg
     2881 gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta aagcgttggc
     2941 ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg gttatgtcga
     3001 ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc cacacaaatg
     3061 gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat aatgtgaaat
     3121 caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg ggcaaggaga
     3181 aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg agctctattc
     3241 actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa aaggaagagt
     3301 ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct gaaggggata
     3361 atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac aattagccaa
     3421 tcagcagcaa gctctgccag ccagaaggac acataaaaga agaacattgc agcagaggca
     3481 cagaaggagc ctgcgaggag ctgggaaata cacacacaac agcagaacca caacaccctc
     3541 ccctggacac accctactgg ggatcactgc ttttcttttt ttctgaacca tcgcccacgc
     3601 cacacggaga gaaatctctc tctcatcatc atcctgaaga aaaccccctt atcctcattt
     3661 tcacactgct gaggaaaacc tacaatcgca cgggctgaga tttcctggcg aagactgtcc
     3721 tttttccttt ttcttttttt ttcttttcct ttggaaactg acatttgcat ttctccattc
     3781 caagccacgg cgtaataata tctgcaatcc agcctgaaga cctgcaaatc gaaggaccta
     3841 gatcactatc atctttgtac gtcaagaatg gttactgtac gtataacctt tctttctttt
     3901 gctctgacca atatgaaaca ctaaaatcga ttcgagcagc ctctcagcat caattacagt
     3961 gcgtgaaaaa cattcaaata gaggcagcaa atattacatg tgaaaataca ggctggctaa
     4021 atccaagcta atttagaaat gtggtcaaaa cgcatactgg cacgtctaat cgcggattca
     4081 gtaaacaaga ttaacgatta gcccagtgta taagtcatat gatacaggca tgcgcgagag
     4141 catcgctaca cccgagctgg cttcattttc ggaggaaaat caaaacattg ctttctcctg
     4201 ccgtgcgaac cattcgtcat aaaccgtaat acgcaacata catttattac tacatccgtt
     4261 aattagcgat aattagccgt tattaacaaa gagcgctgag gaattcctct caaatagcgg
     4321 aggtgcggcg gaggcagagg ggcgtaaaag ggcacatgcg tgcctggctc aaaaaaggat
     4381 gctccagact gaaactcaga gacaaaacac gatgcctcgg aggctgagag cccatcgaga
     4441 ggaaaggcaa agaaaagggt cgagtcactc agagggggag gggagatatt gtgcatccat
     4501 tggtttgaat tgaagcaggc agaataaaaa tccttgcgat tatgcttcgt gagagatcgc
     4561 gagagaggaa aaggctaaat gccacgtatg caaatggata aatgtcatct atttcttcgc
     4621 gagtagacat tttgtggaca acgagagtgt aaataagcga tatgagggga atacaaaggc
     4681 cttgagggaa agcgttttgg gcgtgtcttg tcgaaaagaa gacagcgatg gcacgcgctc
     4741 agaaccacca tcttcacgta gataatccgc gtgaaaacaa taacaaaact gctcatttta
     4801 acacgagcag gtgtttgaaa ccacccaaaa aagattgtct aaaatattaa gattaattaa
     4861 tgacgtttaa atgtccaggc ctgatataaa tgaagcgtgt agtaagagta atcgcctttg
     4921 tttcttttca gagatacgtt ttcagagatt cagggaatta tatcttcaat aaaataatgt
     4981 ttgatttcat tattttaaag ctttttaaga agtaaaaata cacattattc atttacattt
     5041 tattttattt aaaggcctat ggtcttgaaa ataataagtg gaatatatag gctatatatt
     5101 cgtctaagga cagccggtgt tattttatag tgacctcaga caaaagccga gacaaaacga
     5161 ggcgcttgcc tttcatttta atccttaata gacggtgtga tgaatgaatg aatgaatgaa
     5221 taaatgaatg aatgacgggc ccttcggcat gttgttccgc agtcctccgt gagcacgagt
     5281 ctctaccgca gtgccaagca cataaaaccg ttgagtcaga taaccttctc caggcgcgta
     5341 tacacaattc aaggacaagc acatcacaac tatacacaac acaacacaac cgcctaaacg
     5401 cacgagctca tcaatcgcaa tttaaaggca tgtcagtcaa agaaaaaggc ggcgttattt
     5461 aaaaccaatg ttcaacaata tttgtatgct gactagcccg ggcgcgcacg ctataagatc
     5521 acaaggcctt gttttttccc tttattcaaa atatcattaa ttattacagg tcataaaaca
     5581 aaacaatgca aattacaatg atttaaagac tattgattta acaataatca gttaattata
     5641 gaataatatt ataatatttt acatacaaat gaaagatgac attatttgat gacattgtaa
     5701 ttaaaatata tgcaatatat ttttacgttt ttattaatta ggctattact attattataa
     5761 ttatcattat ttttattatt attattatta ttaaatagtc ttgcgaaata agagtgaagg
     5821 cagttgcaat gttcattgtt cccggagagt cccgcagcct ctggcctcaa cacaaagcgc
     5881 tactgtttac attaatattc atagtgctgc catggtctct gtagaggagg aacgggtggc
     5941 tcttttgttc gctagtgtca atacgtttat tcagcaaaaa gcagctcgga tttgtgaatg
     6001 cccaacaagc acacgcgcac tccaatcaaa ggacgagagg ataacgattt taaacgattc
     6061 gcaaacgtgt ttatttgtaa agacgacatc aagagcttaa tgtccacttg aaaagaaata
     6121 aatatgcccg cggcgccaag gatattttga atgtagtcgt tcatggtgag ccactcccga
     6181 attcagcact ctggagagcg accaaagagg aatagcggaa tagcaactac ttgagccatt
     6241 tctctccgtt tgatggcgtt catgattaaa aatataatca cgtgattttt tatacatata
     6301 taaatatata cataaagtaa cgctttcctt ttgagaatac ataaatattt attcgcgtga
     6361 taaatcaatg atcttatatt tatttgcacg accgaaatta gccaaattca ccaattaaaa
     6421 aaaaaataag ccaacaaaaa agaagggttt atccgttcag ttttgacttg tgtcctgttg
     6481 ttttacagct gccactgtga tcccttaagc tgcagtgaga gtgtggctaa tgccttttgt
     6541 ttaagataat cctgtaatct gttaccgaaa cggcctattg acaagccggc attcacattt
     6601 cagtcaggag gcccgtccag acatgtacat ttatgaatta cgaatgataa aattaagatc
     6661 tgcattaggc gtcagaaatg tcacgaacac ccattcattt cccacagcca ataatggatc
     6721 atgctgggaa gcgctatcct cgcagtctca aaattaatgt tgacatgttg cgtagagcta
     6781 cagattagat cagccatatg ctttatgtgt ttacctcagc ggatcttcag caagatgtgt
     6841 ttattttaag aaaatggctt cctcgctgcc tgcacagggg cattaaggaa gtgacgtgag
     6901 cgtcctcaca gtcaaattac tttatctcat gctgctttca ggcctctgct tattttatta
     6961 ttattatttt aaattagggg gaatcacggt ggcgttgtgg gtagggtagc gcgatcacct
     7021 tacagcaaga aggtcgctgg ttcgagctct ggctgggtca gttggcattt ctgtgtggag
     7081 tttaaattga attgaaattg aataaactaa attggcccta gtgtatgtgt gtgaatgtaa
     7141 gtgtgcatgg gtgtttccca gtgttgggtt tgactggaag agcatccgct tcgtaaaaca
     7201 tatgctggat aagttggcgg ttcatttcac tgtggtgacc gctgattaat aaagggacta
     7261 agctgaaaat gaaatgaatt aatacatttt aaatgagctt ttgctgacat gtttatgatt
     7321 acaaatagtg aaattgtatg ttaatttacg ataagtgcat tgttctaaat tcatttttat
     7381 gtagcagctg gtttttcttc catttgtgtc attcaacaga acatttttct aaaaaagtga
     7441 acttaaattt tctcctccct ctgacaaggc ttgatggtaa tttcagccga ctctaattac
     7501 agactcacta aaaggaatcg gtgcagtcat ttatctttat cagcgcgctg gtgggaaggt
     7561 aataggtttt gcttatgagt tgtttgcgtg cgagtctggg tccttgtgta ggcgatgctg
     7621 atgcactgca ttaaatattg agggagaagt cgctcaaaaa tagcaaagta caggctgggg
     7681 caggaacgtc aaagcccgct cagtggccac gtttcttgtt tcgtaagtcc tatttttaat
     7741 agcgataatt caggctcagt agaaggaact ccagcactat aagcagtctc tcgcctgtct
     7801 cggcgagtta tgtattttgg gatgaagtgc tcttccacct ctacctctgc tgtgctggcg
     7861 ccagtatctg ggctgtaaga tagtgtacgt gtcagtctgt gtggccgaag agcctgtgtg
     7921 ttgtgtaact cactacattc gtcgctgtgg atggctgact tgccatgggg aggtttactt
     7981 cagttcacga atcacattcg ccacgatctt gtcactttgt gtcaagactc gctctctgtg
     8041 tgtcaacagc tctgctgttt gaggttgaaa ttctgaactt gagatatatc ccaggatata
     8101 ttagggctac tgccaacgga aaaagcaaat ggtgccatgg ccatttgatt gatcaaacaa
     8161 acaccctttt gcttgggtga cagtgaaatg tcagatccat gtttgtactg ctttaataac
     8221 ttgcctctct gagctttctc cttgtcagtg tcaatttgcc tgagatataa attccggttg
     8281 ccccgaaagc ttattttatt aaaaaaaagc taacatgcac tcatacaacg tgcgcacata
     8341 catgtgaggc ttgctaaaag tcaagaagca agccaatagc aagagtcgtc agactgctgt
     8401 tacctgggca acctggttag aggtaaggct caaggcaaga ataaatcatt ccactgaaga
     8461 gacacagcgt tacagcgaga tgagagcaag aggcgatagt gaagggagaa caaaagaaca
     8521 gcctacccac actagtgcag atctacctgt ttgagctact gattgttcag tttgattaga
     8581 tatcagacaa ttctgctatc tccaattgca ttgctgcatg aattcctaga aatcatttta
     8641 atcaccaaaa actagtaggg acacttgtgg cgtatatgat tctgcctgtt gattgtgggg
     8701 atccaacaga aagatcatga atgcattgtt aattaagtcg gtgagacacc ctgttccacc
     8761 ctaggggccg ttctggggaa agagtgcttt cagtcagcta aagatgacta atatgtgaac
     8821 atatattact acacatgcca atttgtacat tctggatagt taccagccct gggaaaacat
     8881 cgaaaaaaat aaatcaacat agataaaatt ggcaaatccc ttctggtacg atgcagagtg
     8941 cttgctctga gaacttgtgc attgtgacag aaagcgaccg ttaaaaactg aaagtagcat
     9001 ctacaacctg catacatctg ttctttgaaa atcccctgta ggccaaatta agcatcacgc
     9061 tgtgctccac agcaacacgg atgaaccgaa aacccagaca caaacgcaca cacttttcac
     9121 ataaacaagg tttagttatg ctagctatgc tttttcgtcc tccctttgtc atcagcggcg
     9181 agtgacgtaa cacagtttga ccctggacag cgtaaagggg gggttgggaa tgagtgaaag
     9241 gactgtgaca tctgcagggc caggggctga tggagagagc tgaagtgagg gaggtcagag
     9301 gaggaggaag ggtgggatat tgtcctcctc aggccctatc cctctcagac gcattgattt
     9361 ttaacctctc agcgagaatg ccaagcatta cacccctcaa attatggttc ctcacccaac
     9421 ctgttatatt ttccacctgc agataattac cggtatggct gacgtggaaa ccgagaccgg
     9481 catgattgca cagtggattg tctttgctat tatggctgct gctgctattg cttttggagt
     9541 ggctgtgcac tttcggcctt cagagctgaa gagcgcatac tatatcaaca ttgccatctg
     9601 cactatcgcc gctaccgctt actatgcaat ggccgtgaac taccaggacc tgacaatgaa
     9661 tggtgaaagg caggtggtct acgcaagata tattaactgg gtgctgacca caccactgct
     9721 cctgctcgat ctcatcgtca tgaccaagat gggcggagtg atgatttctt gggtcatcgg
     9781 cgcagacatt ttcatgatcg tgtttggtat tctgggcgcc ttcgaggatg aacacaagtt
     9841 caaatgggtg tactttatcg ctggatgtgt gatgcaggca gtcctgacat acgggatgta
     9901 taacgccact tggaaagacg atctgaagaa aagccccgag taccatagct cctatgtcag
     9961 tctgctcgtc ttcctgtcaa tcctctgggt gttttatcct gtcgtgtggg ctttcgggtc
    10021 tggtagtggc gtgctgtccg tcgacaatga ggccattctc atgggaatcc tggatgtgct
    10081 cgctaagcca ctgtttggaa tggggtgcct cattgcccat gagactatct tcaagatcgg
    10141 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
    10201 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
    10261 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
    10321 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
    10381 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
    10441 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
    10501 tattgcattt atggagttca tccaccctat cccgacctgg gacgaatggc cagaatttgc
    10561 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
    10621 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
    10681 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
    10741 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
    10801 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
    10861 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
    10921 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
    10981 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccacta agagcaggat
    11041 caccagcgag ggcgagtaca tccccctgga ccagatcgac atcaacgtgt tctgctacga
    11101 gaacgaggtg caaagtcagc ctatcctgaa cacaaaggaa atggctccac agtctaagcc
    11161 tcccgaagag cttgagatgt ccagtatgcc aagtcccgtg gctcccctcc ctgccaggac
    11221 tgaaggagtg attgacatga ggagtatgtc atctattgat agcttcatct cttgcgcaac
    11281 agatttcccc gaggctactc gattctaacc ggtggatcct ggccgtcgtt ttactgtacc
    11341 atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
    11401 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
    11461 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
    11521 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
    11581 gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
    11641 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
    11701 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
    11761 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
    11821 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
    11881 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
    11941 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
    12001 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
    12061 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
    12121 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
    12181 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
    12241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12361 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    12421 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    12481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13081 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    13441 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    13501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    13561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    13621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    13681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    13741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    13801 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    13861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    13921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    13981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    14041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    14101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    14161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    14221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    14281 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    14341 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtg
//
