LOCUS       sequence-225136-        7522 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter 
            for multiplexed electron microscopy labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Zhang Q, Lee WA, Paul DL, Ginty DD
  TITLE     Multiplexed peroxidase-based electron microscopy labeling enables 
            simultaneous visualization of multiple cell types.
  JOURNAL   Nat Neurosci. 2019 Mar 18. pii: 10.1038/s41593-019-0358-7. doi: 
            10.1038/s41593-019-0358-7.
  PUBMED    30886406
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Mar 18. pii: 10.1038/s41593-019-0358-7. doi: 
            10.1038/s41593-019-0358-7."
COMMENT     Sequence Label: pAAV-FDIO-SYP-HRP
FEATURES             Location/Qualifiers
     source          1..7522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        231..1409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          462..1400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1357..1377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1440..1473
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      3472..3481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3507..3540)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3649..4237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3702..3722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4120..4131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4269..4745
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4468..4487)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4785..4925
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4785..4914
                     /label=AAV2 ITR
     rep_origin      5000..5455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5087..5106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5297..5318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5472..5491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5591..5613
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5651..5669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5737..5841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5842..5910,5911..6702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6060..6079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6873..7461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7362..7381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga
      181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1381 gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc gactccggag
     1441 aagttcctat tctctagaaa gtataggaac ttcgcagaat ggtagctgga ttgtagctgc
     1501 tattagcaat atgaaacctc ttggaagttc ctattcttca aatagtatag gaacttcgcc
     1561 tcaaagccgc gtgacgagct agcccatggg aattttacat ctgattggag aaggaggtgg
     1621 gcgcaccctg ttggccgtag ccttgctgcc catagtcgcc ctgaggcccg tagccaccgc
     1681 caccgccgct ggctggctgc ccgtaatcgg gttgataacc accctgaggc ccgtaggagt
     1741 cctggggccc atagcctccg gggccctgcc catagcccgc atcgccgtag gcatcgccag
     1801 gagctggttg cttttctggg gcgcctggag gtgcgcgcat gaatggggcg gcccagcctg
     1861 tctccttgaa cacgaaccat aggttgccaa cccagagcac caggttcagg aagccaaaca
     1921 ccaccgaggt gttgagtcct gaagtcacag ggtccctcag ttccttgcat gtgattcgag
     1981 ctccaccggt agagttgctg ttgaccactc tgcagttcag acgaatctgg ccttgggtac
     2041 ccgtcagagg ggtaatgtta cccatacggt ccatggcttc cacgaaggcg ttaaagaagg
     2101 tttgagtaga gttagcaaaa cttctcacca gtgggatggt gtcagtggcg tttggactgc
     2161 taaacagttc ttgatcactc tgtatcaggc ctttctgctc ctctagattc acatagtact
     2221 tgttatcgaa gatggttggg gtccgcagat caaagtccac tagtgcactg aggttgccat
     2281 tcagtgggca caagcctctc agtgtctgga gatacgtagt gttcagcgtg gggtcaggta
     2341 acccagtgtt gctgaaattg tagagcctat ccatgatgaa cctacactgg ttctttccaa
     2401 atgtgtgtcc tccggacaga gccacaaggt cactcgagcg attcagaccc acgtttctaa
     2461 agctatcctt cagctggggc agggtgaaga atggagcagg caagttggcg ttggccagat
     2521 ctaggaatgc ctgtagggag tcacgtcgac cgagcggcac tctccaggac ggtccgcctg
     2581 caagagtcac gctctgttgc gcagctatag tcagcaggtc tgcacaactg actgttcgtg
     2641 ggcatgctga ctcaacggca gccttcatgc gatcgatcac tggaaagccc ctggcgctgt
     2701 tagcgttccc gaatgcatcc ttttcagtgc ggaaactggt ggtgttgtcc agtaatatgc
     2761 tagcgtcgca accattcacg aagcagtcat ggaagtgcag acgtaatatt gaagcagcga
     2821 tcctgggatc ggatctgagc tcgttgacga ttgtgtcgcg aacgatgttg gacacgttgg
     2881 gacagctatt gtcgtagaat gtaggggtta actgggtggc tagatccata tctgtctggc
     2941 ggcacatagg catctccttg ataatgttct ctgggtcagt ggccatcttc acatcggaca
     3001 ggcctttggc ccaggcggat gagctaacta gccacatgaa agcgaacact gctgtggcca
     3061 ggaagtccat cattggccct ttgttgttct ctcggtactt gttctgcagg aagatgtagg
     3121 tggccagggc ccccatggag tagaggaagg caaacacagc cacggtgaca aagaattcag
     3181 ccgaggagga gtagtcacca actaggaaga tcttggtagt gcccccttta acgcaggagg
     3241 gtgcatcaaa gtacacttgg tgcagcctga atgggtactc aaattcgact tcgatgttga
     3301 gggcactctc cgtcttgttg gcacactcca cgctcagccg aagctctccg gtgtagctgc
     3361 cgcacgtagc aaaggcgaag atggcaaaga cccactgcag caccttcacg aagccaaggg
     3421 gctccttgac cacccggaac tgacccccag ccaccagctg attcaccacg tccatggtgg
     3481 caattcgtga gcgtcggttg aacagcgaag ttcctatact ttctagagaa taggaacttc
     3541 ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga gaagttccta
     3601 tactatttga agaataggaa cttcaatccc gatatcaagc ttatcgataa tcaacctctg
     3661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
     4081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4201 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag
     4261 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     4321 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     4381 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     4441 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     4501 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     4561 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     4621 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     4681 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     4741 tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     4801 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4861 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4921 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4981 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5041 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     5101 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5161 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5221 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5281 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     5341 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     5401 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5521 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5581 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5641 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5701 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5761 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5821 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5881 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5941 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6001 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6061 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6121 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6181 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6241 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6301 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6361 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6421 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6481 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6541 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6601 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6661 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6721 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6781 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6841 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6901 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6961 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7021 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7081 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7141 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7201 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7261 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7321 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7381 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7441 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7501 tgctggcctt ttgctcacat gt
//
