LOCUS       sequence-225139-       11551 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express NuMA(1-505)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK664
FEATURES             Location/Qualifiers
     source          1..11551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      4761..4770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4767..5498
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             5574..5903
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    6053..6134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6156..6666
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             6703..7722
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6703..7722
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(8032..8049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8038..8262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9363..9383)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9363..9381)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9402..9418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9402..9418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9415..9437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9426..9442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9450..9456,9457..9474,9475..9480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9495..9516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9804..10392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9884..9903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10563..11354,11355..11423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11186..11205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11424..11528)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct ggaggtggat
     4741 ctacgcgtgg aggtggatct gccaccatgg tgtctaaggg cgaagagctg attaaggaga
     4801 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     4861 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     4921 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     4981 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     5041 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     5101 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     5161 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc gagatgctgt
     5221 accccgctga cggcggcctg gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg
     5281 gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca
     5341 agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag
     5401 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     5461 tggggcacaa acttaatggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     5521 aagcttcgaa cgaattcggc attgatctga gcggcctgac cctgcaggaa ttcagctccc
     5581 cgaaacgccc taagctgctg cgtgaatatt acgattggct ggttgataac agctttaccc
     5641 catatctggt ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag
     5701 acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg
     5761 attttatcca gttcaacgcc cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg
     5821 gtgccgctct ggccatttac gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag
     5881 aaatctatga cgagctgaat attggttaaa ccggtggatc cagatctctg cagcatatgt
     5941 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     6001 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     6061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6121 atttttttca ctgccgacgt caggtggcac ttttcggggt tggggttgcg ccttttccaa
     6181 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     6241 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     6301 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     6361 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     6421 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     6481 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     6541 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     6601 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     6661 ccccaggggg atccaccgga gcttaccatg gatagatccg gaaagcctga actcaccgcg
     6721 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     6781 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     6841 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     6901 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     6961 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     7021 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     7081 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     7141 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     7201 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     7261 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     7321 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     7381 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     7441 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     7501 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     7561 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     7621 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     7681 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac
     7741 aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc
     7801 gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg
     7861 gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc
     7921 agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat
     7981 cgccgcccgt gcctgccgat tgggtgcaga tctctagagc tcgctgatca gcctcgactg
     8041 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8101 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8161 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8221 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8281 ccagctgggg ctcgatgagg gagctgcggt ggaggaggtg gagagaaggc cgcacccttc
     8341 tgggcagggg gaggggagtg ccgcaatacc tttatgggag ttctctgctg cctcccgtct
     8401 tgtaaggacc gccctgggcc tggaagaagc cctccctcct ttcctcctcg cgtgatctcg
     8461 tcatcgcctc catgtcgagt cgcttctcga ttatgggcgg gattcttttg cctaggctta
     8521 aggggctaac ttggtccctg ggcgttgccc tgcaggggag tgagcagctg taagatttga
     8581 ggggcgactc cgattagttt atcttcccac ggactagagt tggtgtcgag gttattgtaa
     8641 taagggtggg gtagggaaat ggagcttagt cattcacctg gggctgattt tatgcaacga
     8701 gactgcggat tatcactaat catttttgga gcatttttct agagacagac ataaagcatg
     8761 atcacctgag ttttatacca tttgagaccc ttgctgcacc accaaagtgt agcatcaggt
     8821 taaatcttaa tagaaaaatt ttagcttttg cttgagaaac cagtgcttcc ctccctcacc
     8881 ctctctcccc aggctctcta cccctttgca tccctaccag gcatcttagc aactctcact
     8941 catacttgat cccattttcc atttgttgta cttgctcctc cagtattcag acatagcact
     9001 agctttctcc ctctcttgat cttgggtagc ctggtgtctc gcgaaaccag acagattggt
     9061 tccaccacaa attaaggctt gagctggggc ttgactctta cccagcagtg cttttattcc
     9121 tccctagttc acgttcttaa atgtttatct tgattttcat tttatccttt ttccttagct
     9181 gggattctgt ccctgaccgt cttcacagtc caggtgatct tgactactgc tttacagaga
     9241 attggatctg aggttaggca acatctccct ttttcttcct ctaaatacct ctcatttctg
     9301 ttcttaccag ttagtaactg atctcagatg cctgtgtgat agctggtacc cagcttttgt
     9361 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     9421 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
     9481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10561 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10621 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10681 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10741 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10801 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10861 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10921 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10981 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11041 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11101 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11161 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11221 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11281 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11341 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11401 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11461 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11521 gttccgcgca catttccccg aaaagtgcca c
//
