LOCUS       sequence-225408-        8301 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral transduction and expression of PARK7C53A into any 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLD-puro-Cc-PARK7C53A-VA
FEATURES             Location/Qualifiers
     source          1..8301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1157..1181)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1261..1284
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1321..1344
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1357..1374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1384..1404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1417..1437
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1450..1515
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1563..1680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1729..2239
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2158..2176
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2261..2860
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2261..2280)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2757..2777
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2988..3221
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3293..3414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3330..3349)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3384..3403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3454..3589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3609..3628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3610..3628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3638..3655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3638..3654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3647..3669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3796..4251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3883..3902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4093..4114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4277..4381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4382..4450,4451..5242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4600..4619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5413..6001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5902..5921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6155..6172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6289..6310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6325..6330,6331..6348,6349..6355)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6363..6379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6368..6390
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6387..6403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6387..6403
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6422..6442
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6424..6442
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6470..6696
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6697..6877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6924..7049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7542..7775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7960..8004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgtac gcgtacaagt ttgtacaaaa aagttggcat ggcttccaaa
      601 agagctctgg tcatcctggc taaaggagca gaggaaatgg agacggtcat ccctgtagat
      661 gtcatgaggc gagctgggat taaggtcacc gttgcaggcc tggctggaaa agacccagta
      721 cagtgtagcc gtgatgtggt cattgcccct gatgccagcc ttgaagatgc aaaaaaagag
      781 ggaccatatg atgtggtggt tctaccagga ggtaatctgg gtgcacagaa tttatctgag
      841 tctgctgctg tgaaggagat actgaaggag caggaaaacc ggaagggcct gatagccgcc
      901 atctgtgcag gtcctactgc tctgttggct catgaaatag gttttggaag taaagttaca
      961 acacaccctc ttgctaaaga caaaatgatg aatggaggtc attacaccta ctctgagaat
     1021 cgtgtggaaa aagacggcct gattcttaca agccgggggc ctgggaccag cttcgagttt
     1081 gcgcttgcaa ttgttgaagc cctgaatggc aagggggtgg cggctcaagt gaaggctcca
     1141 cttgttctta aagactaccc aactttcttg tacaaagtgg ttgattctag agcgatcgcc
     1201 agagagctgt tcaacctgct gggcgagaac cagccccccg tggtgatcaa ggcgatcgcc
     1261 tggagccacc ctcagttcga gaagggagga ggaagcggcg gaggcagcgg aggaggaagc
     1321 tggagccacc cgcagttcga gaaaggcgga agcggccatc accatcacca tcaccctgca
     1381 ggagaaaatc tgtacttcca ggggagagga ggcagcgaga acctgtactt tcaggggcct
     1441 gcaggaagag actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     1501 gatgacgatg acaagtagtt cgaattctcg acctcgagac aaatggcagt attcatccac
     1561 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1621 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1681 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt
     1741 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     1801 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     1861 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     1921 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     1981 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2041 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2101 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2161 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2221 caccgacctc tctccccagg gggatccacc ggagcttacc atgaccgagt acaagcccac
     2281 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     2341 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     2401 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     2461 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     2521 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     2581 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     2641 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     2701 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     2761 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     2821 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     2881 gcgcccgacc gaaaggagcg cacgacccca tgcatcggta cctttaagac caatgactta
     2941 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3001 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3181 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     3241 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     3301 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3361 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3421 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3481 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3541 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     3601 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     3661 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3721 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3781 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4141 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4201 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     4261 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4381 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4441 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4501 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4561 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4621 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4681 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4741 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4801 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4861 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4921 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4981 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5041 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5101 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5161 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5221 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5521 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6181 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6241 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6301 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6361 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     6421 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     6481 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     6541 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     6601 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     6661 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     6721 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6781 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6841 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6901 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6961 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7021 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7081 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7141 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7201 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7261 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7321 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7381 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct
     8281 cgagcggccg cccccttcac c
//
