LOCUS       sequence-225599-         600 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses C-terminus of split luciferase fused to P3 coil, PPVs 
            cleavable linker and autoinhibitory coil AP4/for split 
            protease-cleavable orthogonal Coiled-coil (SPOC) logic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: AP4mS_PPVs_P3_cLuc
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    22..43
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             532..558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtaacggccg ccagtgtgct ggaattcgcg gccgcttcta gagccaccat gtcacccgag
       61 gacgagcttc aatctaatga agaggagttg cagcaaaatg aacaaaagtt gcaacaaatt
      121 aagcaaaaac ttcaatctat caaatacggt ggcggctctg gcggcggcaa cgtggtggtg
      181 caccaggccg gcggctccgg aggctcttcc cccgaggacg aaatccaaca gctcgaagaa
      241 gagatagctc aacttgaaca aaaaaacgca gcactgaaag agaaaaacca ggcacttaaa
      301 tacggtggcg gctctggcgg cggctccgga ggctctacca tgaccgagaa ggagatcgtg
      361 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
      421 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
      481 attaaggcca agaagggcgg caagatcgcc gtgaactcta gtggttctgg atacccatac
      541 gatgttccag attacgctta aactagtagc ggccgctgca ggggccctat tctatagtgt
//
