LOCUS       sequence-22575-e         836 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hirota J, Omura M, Mombaerts P
  TITLE     Differential impact of Lhx2 deficiency on expression of class I and 
            class II odorant receptor genes in mouse.
  JOURNAL   Mol Cell Neurosci. 2007 Apr . 34(4):679-88.
  PUBMED    17350283
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci. 2007 Apr . 34(4):679-88."
COMMENT     Sequence Label: pMOR22-2-ISH
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(662..680)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(663..680)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(698..714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(698..714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(711..733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(746..752,753..770,771..776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    791..812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt aaggctgtag
       61 ataaagggat tcagaaaagg tgggactaac aggtaaaggt ctgacaggag gatgtgcaca
      121 taggggggta tgtgagcccc aaacctgtgt gtgaagaaag agaagaaggc cagaaggtag
      181 aactgcagga agacacagat gtgagcaatg caggtattga aggctttgaa tcgagcctcc
      241 ttctggggca gctggaagac cgtgatgaag attcgcacat aggagaaggt aatgaagact
      301 atgtcaaacc ctaagatggc aaaggcaaca aggaggccac atatcttgtt gattctgata
      361 tcttgagctg ccaatttcac aatggccatg tgttcacagt acgagtggga gataatggta
      421 gttcgaaagt acttaaggcg acatttgatg agcaatatca atggaaatgt ggtaatgaga
      481 gacctcagta atgcaccagc tccaaggcaa gtggtgagtt gtggagagaa gatagtagca
      541 tgtctgaggg ggttgcacat agccacatac ctatcatacg caatcactag tgaattcgcg
      601 gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
      661 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgct
//
