LOCUS       sequence-226474-       15443 bp    DNA     circular SYN 14-APR-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-145
FEATURES             Location/Qualifiers
     source          1..15443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(91..96,97..113,114..119)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             308..841
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             1203..1898
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1336..1354)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1604..1623
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1919..2013
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2057..2716
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2123..2142)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2781..2867
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  2978..3143
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(3576..4526)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4574..4796
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     5479..5498
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            5536..5645
                     /note="incP origin of transfer"
     primer_bind     5668..5687
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(5816..6607,6608..6676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6439..6458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6677..6768)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(6787..6814)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6906..6992)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     7145..7162
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(13853..14137)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(13911..13930)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             14164..15042
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 actgccagga attggggatc ggattcgagc tttagtacct agatttagat gtctaaaaag
       61 cggggtaccg aggacgcgtc aattctcgaa ttgacataag cctgttcggt tcgtaaactg
      121 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      181 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg
      241 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      301 agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
      361 gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa
      421 atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc
      481 ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc
      541 gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccaa
      601 gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg
      661 gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg
      721 tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac
      781 aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta
      841 aagctcgcac gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg
      901 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
      961 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     1021 cgatagctag actgggcggt cttatggaca gcaagcgaac cggaattgcc agctggggcg
     1081 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     1141 atctgatggc gcaggggatc aagatctgat caagagacag gatactagtg gaggaagaaa
     1201 aaatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg
     1261 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1321 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg
     1381 acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca
     1441 tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1501 tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga
     1561 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga
     1621 agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg
     1681 gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga
     1741 ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt
     1801 tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg
     1861 gccgccactc caccggcggc atggacgagc tgtacaagta agtaattagc tgagcttgga
     1921 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1981 ggttgccgcc gggcgttttt tattggtgag aatccaagct agcttggcga gattttcagg
     2041 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2101 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2161 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2221 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     2281 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2341 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2401 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2461 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2521 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2581 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2641 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     2701 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggggtaat
     2761 gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2821 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgctaggag
     2881 cttgcggccg ccaggggatc tgattaatta aaacgtggct tactaggatc cgatatcatt
     2941 taaatctagg gataacaggg taatactagt gtcgaccaac cagataagtg aaatctagtt
     3001 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     3061 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     3121 cccaactatt ttgtccgccc acaggctgaa agcgctattt cttccagaat tgccatgatt
     3181 ttttccccac gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca
     3241 gcatcgcctg tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt
     3301 gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta
     3361 catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa
     3421 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     3481 acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg
     3541 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     3601 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     3661 tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     3721 gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg
     3781 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     3841 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     3901 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     3961 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     4021 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     4081 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     4141 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     4201 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     4261 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     4321 acaccataag cattttccct actgatgttc atcatctgaa cgtattggtt ataagtgaac
     4381 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     4441 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     4501 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     4561 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     4621 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     4681 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     4741 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     4801 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     4861 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     4921 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     4981 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     5041 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     5101 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     5161 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     5221 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcgggga
     5281 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     5341 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     5401 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     5461 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     5521 gccggtgatg ccgccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     5581 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     5641 gccggcggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5701 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     5761 gctacagggc gcgtaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5941 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6121 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6421 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaaag agtttgtaga
     6781 aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagtttat
     6841 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc
     6901 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     6961 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
     7021 gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
     7081 aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg
     7141 ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac agccaagctt
     7201 gcatgcctgc aggtcgactc tagaggatcc cctaaaaata atgaccgagc tagctctaac
     7261 tgtgggatgt gataacgagt cttgctatat ggtgtttcag cttcgtaaat aaatgaactc
     7321 tgcgctccat ccttgttgcg tacggaagca aaggacgaga gattaggaaa atcgattatt
     7381 ttggctcgtt gaagatccga tgcttcaaac tcaatcacac gatccgcctt atttatttgc
     7441 tttgttgtac tgttaaccac cttagctcga cttaataaag gcaaatgagt aaatcgagtc
     7501 catttccgtt cttgcattgg attaaaccaa acaaaaatac gccactcctt atgacccgag
     7561 ttacggaata aatgaccaat atgtacaacc tgcacattgt cattaaatgt agctagccta
     7621 accactcact cttccccata aaccatttcc aagaatcttt gtgcttcctc agttatccgc
     7681 tctttagata acgttgcact tcttaataat cgccagcgcc taagctcaac atcttcctgt
     7741 ttaagcttaa taaaagcttg gctaatccgt ctaatttgat aatcttccac actctctgag
     7801 taacgcttta agcaaagcgc taccaaaggc agtttctgta gattttttgc aagagaggtt
     7861 ccgttaggag tttgctttaa cagccagctc gatgtcgctc ttgggtgatc aaggctacta
     7921 tctagacgct ttatgattct taatagttgt cgtacagcaa ttcgatctct ttggttccaa
     7981 tcaactctag gggcgggtgc cagacgctct tgttgatgct gttgattcca gtgaactagc
     8041 caatccctgt catgtcggta aagccaagcg tatagcaccc caccctctaa agactgtctt
     8101 gccgccttaa ttccttggta tttatgcact agttgctgcc agtctttatg cttaacagat
     8161 aaatcttcag agttaggttg cacagactgg ctaacaggtc ttgtcgttat agagtgctca
     8221 gtaagagcac ttgcctgctg tagcgcttct ataaccgtta gttttggcaa taaggcttgc
     8281 cacacaatac tatgctgtaa ataactaaag gcttttctat gcttacggaa tatacttttc
     8341 agccaacacg tgtccttgtt ctctgccaac tttaaatcca gtttctctag tgcctcatca
     8401 ctaaaagtct gcctcactct ctccgccacc aagtcatgac gaatgtgctt gcttttggtt
     8461 agccctagat cctgcgctaa gcgctgataa aacagcgtcc actgctcaag gcttggggaa
     8521 agctcttgcg ctcgtggagc atctaacaga ggggctatat aagcagctag tgctgttaat
     8581 tgagataggg agtctttggg gtagtctgaa agcagctcag tatgacccaa agcccaaaat
     8641 tgatgtcggt gatcatctac agctctatca aagaagacta aagcaccgtg ttttggacaa
     8701 tatggcaaag cgggcaaata ccaatctcgt tgccaaaagg cttccccata cctatttagc
     8761 tgaagagcaa cgcaatcagg gcagtatcta aagcggttat cgctcttaac tctagaagca
     8821 gcgactccta gcattaaatg caccgcacct tgcgcttggt actccattaa ccgaatagct
     8881 tcgtctcggc gctccttgcc tacaaacgga gcatataaag ggaataaggt atgctcataa
     8941 ataagctgct gaacagcgta acgtcctgtt tgatgtagat gtcttgctat cacacctaaa
     9001 tgcgagggca gacctaaggt agcgaccacc ttgcggttgc catacacctc atccaacagc
     9061 tgcttaggac taacaatccc ttgataaacg cctgcccgtg caatagtgct ataaatcagc
     9121 tcattcgagt acggaacagg aaagtttctc atggctaacc tgcttgttta aataagctcg
     9181 ccatatccac tattacccct ttccctttta accgttcatg catggttttt tcaggttggc
     9241 gttgtgaata gatataacgt aaatccgtat caggcaagct atcccagtcg ctgggcttga
     9301 ctacctttat agccgaaacc tttttactct tggagggctt ttctagttct gacactaccg
     9361 tttcgccttc catcaaccac tgcaaaacaa gaggcagtaa cttttgtctt gtcatcgttg
     9421 gattctggct aaacgctttt ttaatagtgg gaattaacag gcttgaatcg taatcctctt
     9481 tcagcatcag atataaatga cgctgatctt cggtatctaa ctcttgaagg gctttttctt
     9541 ctggtgtttg ttcttgtatc gctgcgatat ctagctgaag ttggattaac cgtttatcaa
     9601 tctcgggaac gactagatca gaataacgag caatgcgttc tgggataccc gagcgtaatg
     9661 cctctagcat ggggtgcaca ggctttaact catcttgata cacttgccgc aataaaccag
     9721 cggtaatacg ctcattgcct aaagctagcg cacggagctg agcgagtaca aaaagtttta
     9781 ctacaatgtc catcactcct tggcttagct cataccacac atcacggacc tcatccgata
     9841 acagcgcatc tttgcgttgt aaaagctgta attgccaaag attatccgta aaagcgatcc
     9901 actcttgatt gggctttcca cgttgcgttt gttgtatagg atcccagaat atagctccaa
     9961 accctgcccc tctacgtgca gaccgcaaat cagcctcaaa aatctctcgt gctttagggg
    10021 taccaatcaa catcactggt acgccaataa tattcaccat cgtcacaaaa aagttcagca
    10081 tctcttgaga tccacccgaa cgagagcggc ttaaatgctg aatttcatca ataaccaaca
    10141 accctaaagc atgtgcattg gctatttgcg acatcaaagc caacatggtt tctataccat
    10201 gacgttttaa gccataacga cgctcatagt tcgagcccaa ggctcgatcc aacgctctga
    10261 aaaaattcaa gcagatttct tttagcgaac cattatgcga gcagtctatt ttcaaataca
    10321 ccacctgctc tacattgagt tcacgatggt aaatcacctg aggatacgtg gctagaatac
    10381 gatgaagaga ggtcgtcttc ccactaccag aacaaccaat taacaataag ctttgtgccg
    10441 tagatcgtgc ctcctcaaag cgaaatgtct ccaactctcc cgtttgaaca cgctcataac
    10501 cattttgtaa atgcttttgt aaatctcctg ttttaggatt tctgcctacg tagccacctc
    10561 gaatcatgac cgaaatacgc tcacttagta gcaaatgcgt acctaatggc tgaaaatagt
    10621 catctggaat acgacaaatg gtatgagctc tgataacacg ggacttttgc aagtcagagg
    10681 aagtaagctg taaagaggat ttcagtgatg cagcactatt cactgactct tgtaatggtg
    10741 gtaaggcctc gataaaagga ttatcacgat aagcctctac ccccgtatca cgataaactg
    10801 cttgaatccg ggtagcactc atgactcatc cttttctggt ggatcctgaa ataattcagg
    10861 cacgtatgtg ggtaggctgt aatcttcttg atcatccgct tccactgcgt tgaaaggaat
    10921 aactttagcc tcatcacctg aagagcttgg cttcaaatgc tccgcacgtt ttttacgctc
    10981 cgaggtcacg gcttcttttt tattagtttt aatctgctta atgcgtgttg atttgggctc
    11041 agtagtactg ggcgttaact tattcgcttt ctgaatggtt tgctgaataa acgcctcaag
    11101 ctccctgcgt ttagttaact catcctgctt cgcattggct ttattgtgtt tttcttgtgc
    11161 ttgtatatcc caaacctccc aaaatgagag acctttaaac tgccgactac gttccgtcag
    11221 attacagcgc caaaatacac ggctgccaac ttgcggaaac aaataaatcg tatcaaccag
    11281 cactgggtca taagccgctt ctaaatgttg aggtctagct atatcagtgc tccgctgcaa
    11341 ccaaccctca cgcagaatct ctgaccccga gtaatacaaa ccccacaaat taacgccaaa
    11401 tgaagaaata gagacctttc ggcgaggcag taacgctact cgcaactgct cttgctccac
    11461 agcccttaaa ctacctgtac gatgctgcat accccattgc catagctgga caggaataga
    11521 cggtaaatct gtaggaaaat cagcatctcg atcgtattta tccatcacca gatggttatt
    11581 tctgaataag atcgtacgca aaataatttg tgtgaactca aataccgaca gagatgcatc
    11641 taacctatag tctgtttcac catggctttt gatccgactg ccctctacaa tgccaggtgc
    11701 aaaggactta aactcggctt gtagtgttct aaaagtgctt tccactatgc ctttagcatc
    11761 gccacgtcta ggtggagcac tttccactcg cacattaaaa ctagaaacta aggcttcgac
    11821 ctgatgactc attaattcgc cacggtccgc tagcaacaca tctggcaaac ctacacacgg
    11881 ccagtctgag ctactaatct caatatcatg ctgggcacaa atggccgttt tgtcagagca
    11941 agcatttaca aaagcctgca tcgccaccac ataagacgga ttttcaaagc cgatataaaa
    12001 gcccgtgatc atccgactaa acacatcaat cacaatgtaa agcgttggtc ttcctatgat
    12061 tttttggcga tcatgatgat ccactaaata aatatcagca atcgtggcat caatctcata
    12121 acgactccca gggcctaacg cctgagaagt ggctgtacta cttaagggtc gtacgtcttt
    12181 tttatatacc cctgctttaa ctctagactt taagcgctga gctttagggt attctcgatc
    12241 ataaaaataa cgaaactgac gtagtgttgg gtaatcctct tggggaatgc gaggaaaata
    12301 ctgagcaaac aagtccacaa atcgtctata ggcaacggtg gtctttgtac ctttttgatt
    12361 taacaggtgc ttttctatgg tcaacctaaa aaggcgttca atctcgggcg ttaccttggt
    12421 tccttcaccc ttaccatatt ctctggctcg gccaatcttt gctgttcctg tcgctgaacg
    12481 tctttcccct ggtgcaccgc tgtttttgta gtcaggaatt aatgcattag gcgtttgacc
    12541 acgctgccag taacggcgta acaacttata aactgtagcc ttagtaacct tatgttcttg
    12601 gaccacatgc tcaacgagtt cgcttctgac tttagggtcg aaacgatcct tactattaat
    12661 aataggaaga atttttcgat aatcctcctc gcgcttctgg aagctgacag aatctacaga
    12721 cggctcttct aaatcaagat gtacataagg gtcatcagtt ctaactaagc ggccttcatc
    12781 taaatactgc attagcaaca actcagccct agcttgtgga actcctttat cagcgcttat
    12841 ttgcatccag acaacttggc catcctctat agccaaaatg cgatacggat cattatcaaa
    12901 tagcacaacc tcattaattt gccacatagc gcaactcctc catattcact acttggctaa
    12961 tacagagatc tgcacactta tttgccctga aagacttata aatattgaac ttaataaaac
    13021 catttgcggt taaggctcgt atctcactca atgttttgcc taactccaaa tcataagcaa
    13081 tatcaacctg cttacagaca ttgataatgt tttcatctcc tttttcttgc aggatatggg
    13141 ccaatgggga tagttgtgct aaaagctccg cagaaacttc ttctgttttc actgaataaa
    13201 gccattcaat attttctttt actacgggat ttatttcttt atcagtaaaa atgaaccaag
    13261 gaatttgctt ttgctgccaa tagcgacgct ctagttctag tttttctaag gtacgctcgt
    13321 cttgtaaggc tgctgcaggt ttgacttgaa tagcaaactg ctcaaaagga ccatctttgc
    13381 agtccactaa aaaatcagta gacataacct gatctacacc acgaataaca ggatgcttaa
    13441 taccactatc tattgcaatc tgcctggtat cactaggtaa taaggggaac tgctcgcgta
    13501 tatctagcac gctgctctcc cactcaagac tgagaaaaac agcaagctct aagtcagata
    13561 gcaaatgatg gactcgtccc gtcttatgag aataaatacg gtgggaacga cctgaagaag
    13621 gaacttcttg tactgttagc catggaatat agtctttacc atgcccttgg ccacgcccct
    13681 ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa
    13741 aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc
    13801 tagtttaaga cttgggtacc gagctcgaat tcgctagccc aaaaaaacgg gtatggagaa
    13861 acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat
    13921 gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga
    13981 ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg
    14041 cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga
    14101 cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa
    14161 agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg
    14221 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg
    14281 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat
    14341 cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc aggagagatt
    14401 catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt
    14461 ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa tacggggttc
    14521 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac
    14581 gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg
    14641 ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta
    14701 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc
    14761 gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta
    14821 gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa gctgcttttg
    14881 agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc
    14941 tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt
    15001 tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aattggtaac gaatcagaca
    15061 attgacggct tgacggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag
    15121 gcacattatg catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat
    15181 cgtggcctgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
    15241 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
    15301 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
    15361 gcccgtcgta ttaaagaggg gcgtggggtc gacgcggccg tggcgcgcct cctaggtgct
    15421 cgagtggcgg ccgctattga ccc
//
