LOCUS       sequence-226819-        6396 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2453..2473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2483..2707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2947..2966)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3012..3067
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3066..3085)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3428..3444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3428..3444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3441..3463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3452..3468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3476..3482,3483..3500,3501..3506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3521..3542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3627..3761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3781..3915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3818..3837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3872..3891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3983..4000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4154..4742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4234..4253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4913..5704,5705..5773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5536..5555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5774..5878)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5909..6288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg atacctacgc cggagcctac gacagacaga gccgggagag
      721 agagaacagc agcgccgcca gccccgccac ccagagaagc gccaacgagg ataaggccgc
      781 cgatctgcag agagaggtgg agagggacgg cggcagattc agatttgtgg gccacttcag
      841 cgaggcccct ggcaccagcg ccttcggcac cgccgagaga cccgagttcg agagaatcct
      901 gaacgagtgt agggccggca ggctgaacat gatcatcgtg tacgacgtgt cccggttcag
      961 caggctgaag gtgatggacg ccatccctat cgtgtccgag ctgctggccc tgggcgtgac
     1021 catcgtgtcc acccaggaag gcgtctttag acagggcaac gtgatggacc tgatccacct
     1081 gatcatgagg ctggacgcca gccacaagga gagcagcctg aagagcgcca agatcctgga
     1141 caccaagaac ctgcagaggg agctgggcgg ctatgtgggc ggcaaggccc cctacggctt
     1201 cgagctggtg tccgagacca aggagatcac ccggaacggc aggatggtga acgtggtgat
     1261 caacaagctg gcccacagca ccacccccct gaccggcccc ttcgagtttg agcccgacgt
     1321 gatcaggtgg tggtggcggg agatcaagac ccacaagcac ctgcctttca agccctccgg
     1381 agggtctggc tccggatcaa gtggtggcag cggtaccatg gccgccagcg acgaagtgaa
     1441 cctgatcgag agcagaaccg tggtgcccct gaacacctgg gtgctgatct ccaacttcaa
     1501 ggtggcctac aacatcctgc ggaggcccga cggcaccttc aacagacacc tggccgagta
     1561 cctggaccgg aaagtgaccg ccaacgccaa ccctgtggac ggcgtgttca gcttcgacgt
     1621 gctgatcgac cggcggatca acctgctgag ccgggtgtac agacccgcct acgccgatca
     1681 ggaacagccc ccctctatcc tggatctgga aaagcccgtg gatggcgaca tcgtgcccgt
     1741 gatcctgttc ttccacggcg gcagctttgc ccacagcagc gccaatagcg ccatctacga
     1801 caccctgtgc agacggctcg tgggcctgtg caaatgcgtg gtggtgtccg tgaactaccg
     1861 cagagccccc gagaaccctt acccctgcgc ctacgatgat ggctggatcg ccctgaactg
     1921 ggtcaacagc agaagctggc tgaagtccaa gaaagacagc aaggtgcaca tctttctggc
     1981 cggcgatagc agcggcggca atatcgccca taacgtggcc ctgagagccg gcgagtctgg
     2041 catcgatgtg ctgggcaata tcctgctgaa ccccatgttc ggcggcaacg agcggaccga
     2101 gagcgagaag tctctggacg gcaagtactt cgtgaccgtg cgggaccggg actggtactg
     2161 gaaggccttt ctgcccgagg gcgaggacag agagcacccc gcctgcaatc ccttcagccc
     2221 cagaggcaaa agcctggaag gcgtgtcctt cccaaagtcc ctggtggtgg tggccggcct
     2281 ggacctgatc agagattggc agctggccta tgccgagggc ctgaagaaag ccggccagga
     2341 agtgaagctg atgcacctgg aaaaggccac cgtgggcttc tacctgctgc ccaacaacaa
     2401 ccacttccac aacgtgatgg acgagatcag cgccttcgtg aacgccgagt gccccaagaa
     2461 aaagcggaag gtgtgagaat tcctgtgcct tctagttgcc agccatctgt tgtttgcccc
     2521 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2581 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2641 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2701 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2761 tgtagcgtgt accgctggga gttcgtagac ggaaacaaac gcagaatcca agcgcggccg
     2821 cccaggatac atagattacc acaactccga gcccttccac cgctagcgca ctcctcaggt
     2881 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     2941 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     3001 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3061 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     3121 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     3181 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     3241 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     3301 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     3361 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     3421 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3481 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3541 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     3601 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccagtt ccgcccattc
     3661 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3721 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3901 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3961 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4141 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4561 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4801 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4861 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4921 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4981 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5041 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5101 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5161 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5221 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5281 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5341 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5401 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5461 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5521 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5581 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5641 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5701 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5761 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5821 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5881 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
     5941 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     6001 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     6061 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     6121 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     6181 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     6241 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     6301 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     6361 tttatttttt aattattttg tgcagcgatg ggggcg
//
