LOCUS       sequence-226887-       15921 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14010..14019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14714..14743
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14744..14767
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14857..14877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15275..15286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15511..15645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15548..15567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15602..15621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15674..15839
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5221 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5281 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
     5341 acaacagcag caacagcaac aacagcagca acagcaacaa ccgccaccgc cgccgccgcc
     5401 gccgccgcct cctcagcttc ctcagccgcc gccgcaggca cagccgctgc tgcctcagcc
     5461 gcagccgccc ccgccgccgc ccccgccgcc acccggcccg gctgtggctg aggagccgct
     5521 gcaccgacca aagaaagaac tttcagctac caagaaagac cgtgtgaatc attgtctgac
     5581 aatatgtgaa aacatagtgg cacagtctgt cagaaattct ccagaatttc agaaacttct
     5641 gggcatcgct atggaacttt ttctgctgtg cagtgatgac gcagagtcag atgtcaggat
     5701 ggtggctgac gaatgcctca acaaagttat caaagctttg atggattcta atcttccaag
     5761 gttacagctc gagctctata aggaaattaa aaagaatggt gcccctcgga gtttgcgtgc
     5821 tgccctgtgg aggtttgctg agctggctca cctggttcgg cctcagaaat gcaggcctta
     5881 cctggtgaac cttctgccgt gcctgactcg aacaagcaag agacccgaag aatcagtcca
     5941 ggagaccttg gctgcagctg ttcccaaaat tatggcttct tttggcaatt ttgcaaatga
     6001 caatgaaatt aaggttttgt taaaggcctt catagcgaac ctgaagtcaa gctcccccac
     6061 cattcggcgg acagcggctg gatcagcagt gagcatctgc cagcactcaa gaaggacaca
     6121 atatttctat agttggctac taaatgtgct cttaggctta ctcgttcctg tcgaggatga
     6181 acactccact ctgctgattc ttggcgtgct gctcaccctg aggtatttgg tgcccttgct
     6241 gcagcagcag gtcaaggaca caagcctgaa aggcagcttc ggagtgacaa ggaaagaaat
     6301 ggaagtctct ccttctgcag agcagcttgt ccaggtttat gaactgacgt tacatcatac
     6361 acagcaccaa gaccacaatg ttgtgaccgg agccctggag ctgttgcagc agctcttcag
     6421 aacgcctcca cccgagcttc tgcaaaccct gaccgcagtc gggggcattg ggcagctcac
     6481 cgctgctaag gaggagtctg gtggccgaag ccgtagtggg agtattgtgg aacttatagc
     6541 tggagggggt tcctcatgca gccctgtcct ttcaagaaaa caaaaaggca aagtgctctt
     6601 aggagaagaa gaagccttgg aggatgactc tgaatcgaga tcggatgtca gcagctctgc
     6661 cttaacagcc tcagtgaagg atgagatcag tggagagctg gctgcttctt caggggtttc
     6721 cactccaggg tcagcaggtc atgacatcat cacagaacag ccacggtcac agcacacact
     6781 gcaggcggac tcagtggatc tggccagctg tgacttgaca agctctgcca ctgatgggga
     6841 tgaggaggat atcttgagcc acagctccag ccaggtcagc gccgtcccat ctgaccctgc
     6901 catggacctg aatgatggga cccaggcctc gtcgcccatc agcgacagct cccagaccac
     6961 caccgaaggg cctgattcag ctgttacccc ttcagacagt tctgaaattg tgttagacgg
     7021 taccgacaac cagtatttgg gcctgcagat tggacagccc caggatgaag atgaggaagc
     7081 cacaggtatt cttcctgatg aagcctcgga ggccttcagg aactcttcca tggcccttca
     7141 acaggcacat ttattgaaaa acatgagtca ctgcaggcag ccttctgaca gcagtgttga
     7201 taaatttgtg ttgagagatg aagctactga accgggtgat caagaaaaca agccttgccg
     7261 catcaaaggt gacattggac agtccactga tgatgactct gcacctcttg tccattgtgt
     7321 ccgcctttta tctgcttcgt ttttgctaac agggggaaaa aatgtgctgg ttccggacag
     7381 ggatgtgagg gtcagcgtga aggccctggc cctcagctgt gtgggagcag ctgtggccct
     7441 ccacccggaa tctttcttca gcaaactcta taaagttcct cttgacacca cggaataccc
     7501 tgaggaacag tatgtctcag acatcttgaa ctacatcgat catggagacc cacaggttcg
     7561 aggagccact gccattctct gtgggaccct catctgctcc atcctcagca ggtcccgctt
     7621 ccacgtggga gattggatgg gcaccattag aaccctcaca ggaaatacat tttctttggc
     7681 ggattgcatt cctttgctgc ggaaaacact gaaggatgag tcttctgtta cttgcaagtt
     7741 agcttgtaca gctgtgagga actgtgtcat gagtctctgc agcagcagct acagtgagtt
     7801 aggactgcag ctgatcatcg atgtgctgac tctgaggaac agttcctatt ggctggtgag
     7861 gacagagctt ctggaaaccc ttgcagagat tgacttcagg ctggtgagct ttttggaggc
     7921 aaaagcagaa aacttacaca gaggggctca tcattataca gggcttttaa aactgcaaga
     7981 acgagtgctc aataatgttg tcatccattt gcttggagat gaagacccca gggtgcgaca
     8041 tgttgccgca gcatcactaa ttaggcttgt cccaaagctg ttttataaat gtgaccaagg
     8101 acaagctgat ccagtagtgg ccgtggcaag agatcaaagc agtgtttacc tgaaacttct
     8161 catgcatgag acgcagcctc catctcattt ctccgtcagc acaataacca gaatatatag
     8221 aggctataac ctactaccaa gcataacaga cgtcactatg gaaaataacc tttcaagagt
     8281 tattgcagca gtttctcatg aactaatcac atcaaccacc agagcactca catttggatg
     8341 ctgtgaagct ttgtgtcttc tttccactgc cttcccagtt tgcatttgga gtttaggttg
     8401 gcactgtgga gtgcctccac tgagtgcctc agatgagtct aggaagagct gtaccgttgg
     8461 gatggccaca atgattctga ccctgctctc gtcagcttgg ttcccattgg atctctcagc
     8521 ccatcaagat gctttgattt tggccggaaa cttgcttgca gccagtgctc ccaaatctct
     8581 gagaagttca tgggcctctg aagaagaagc caacccagca gccaccaagc aagaggaggt
     8641 ctggccagcc ctgggggacc gggccctggt gcccatggtg gagcagctct tctctcacct
     8701 gctgaaggtg attaacattt gtgcccacgt cctggatgac gtggctcctg gacccgcaat
     8761 aaaggcagcc ttgccttctc taacaaaccc cccttctcta agtcccatcc gacgaaaggg
     8821 gaaggagaaa gaaccaggag aacaagcatc tgtaccgttg agtcccaaga aaggcagtga
     8881 ggccagtgca gcttctagac aatctgatac ctcaggtcct gttacaacaa gtaaatcctc
     8941 atcactgggg agtttctatc atcttccttc atacctcaaa ctgcatgatg tcctgaaagc
     9001 tacacacgct aactacaagg tcacgctgga tcttcagaac agcacggaaa agtttggagg
     9061 gtttcttcgc tcagccttgg atgttctttc tcagatacta gagctggcca cactgcagga
     9121 cattgggaag tgtgttgaag agatcctagg atacctgaaa tcctgcttta gtcgagaacc
     9181 aatgatggca actgtttgtg ttcaacaatt gttgaagact ctctttggca caaacttggc
     9241 ctcccagttt gatggcttat cttccaaccc cagcaagtca caaggccgag cacagcgcct
     9301 tggctcctcc agtgtgaggc caggcttgta ccactactgc ttcatggccc cgtacaccca
     9361 cttcacccag gccctcgctg acgccagcct gaggaacatg gtgcaggcgg agcaggagaa
     9421 cgacacctcg ggatggtttg atgtcctcca gaaagtgtct acccagttga agacaaacct
     9481 cacgagtgtc acaaagaacc gtgcagataa gaatgctatt cataatcaca ttcgtttgtt
     9541 tgaacctctt gttataaaag ctttaaaaca gtacacgact acaacatgtg tgcagttaca
     9601 gaagcaggtt ttagatttgc tggcgcagct ggttcagtta cgggttaatt actgtcttct
     9661 ggattcagat caggtgttta ttggctttgt attgaaacag tttgaataca ttgaagtggg
     9721 ccagttcagg gaatcagagg caatcattcc aaacatcttt ttcttcttgg tattactatc
     9781 ttatgaacgc tatcattcaa aacagatcat tggaattcct aaaatcattc agctctgtga
     9841 tggcatcatg gccagtggaa ggaaggctgt gacacatgcc ataccggctc tgcagcccat
     9901 agtccacgac ctctttgtat taagaggaac aaataaagct gatgcaggaa aagagcttga
     9961 aacccaaaaa gaggtggtgg tgtcaatgtt actgagactc atccagtacc atcaggtgtt
    10021 ggagatgttc attcttgtcc tgcagcagtg ccacaaggag aatgaagaca agtggaagcg
    10081 actgtctcga cagatagctg acatcatcct cccaatgtta gccaaacagc agatgcacat
    10141 tgactctcat gaagcccttg gagtgttaaa tacattattt gagattttgg ccccttcctc
    10201 cctccgtccg gtagacatgc ttttacggag tatgttcgtc actccaaaca caatggcgtc
    10261 cgtgagcact gttcaactgt ggatatcggg aattctggcc attttgaggg ttctgatttc
    10321 ccagtcaact gaagatattg ttctttctcg tattcaggag ctctccttct ctccgtattt
    10381 aatctcctgt acagtaatta ataggttaag agatggggac agtacttcaa cgctagaaga
    10441 acacagtgaa gggaaacaaa taaagaattt gccagaagaa acattttcaa ggtttctatt
    10501 acaactggtt ggtattcttt tagaagacat tgttacaaaa cagctgaagg tggaaatgag
    10561 tgagcagcaa catactttct attgccagga actaggcaca ctgctaatgt gtctgatcca
    10621 catcttcaag tctggaatgt tccggagaat cacagcagct gccactaggc tgttccgcag
    10681 tgatggctgt ggcggcagtt tctacaccct ggacagcttg aacttgcggg ctcgttccat
    10741 gatcaccacc cacccggccc tggtgctgct ctggtgtcag atactgctgc ttgtcaacca
    10801 caccgactac cgctggtggg cagaagtgca gcagaccccg aaaagacaca gtctgtccag
    10861 cacaaagtta cttagtcccc agatgtctgg agaagaggag gattctgact tggcagccaa
    10921 acttggaatg tgcaatagag aaatagtacg aagaggggct ctcattctct tctgtgatta
    10981 tgtctgtcag aacctccatg actccgagca cttaacgtgg ctcattgtaa atcacattca
    11041 agatctgatc agcctttccc acgagcctcc agtacaggac ttcatcagtg ccgttcatcg
    11101 gaactctgct gccagcggcc tgttcatcca ggcaattcag tctcgttgtg aaaacctttc
    11161 aactccaacc atgctgaaga aaactcttca gtgcttggag gggatccatc tcagccagtc
    11221 gggagctgtg ctcacgctgt atgtggacag gcttctgtgc acccctttcc gtgtgctggc
    11281 tcgcatggtc gacatccttg cttgtcgccg ggtagaaatg cttctggctg caaatttaca
    11341 gagcagcatg gcccagttgc caatggaaga actcaacaga atccaggaat accttcagag
    11401 cagcgggctc gctcagagac accaaaggct ctattccctg ctggacaggt ttcgtctctc
    11461 caccatgcaa gactcactta gtccctctcc tccagtctct tcccacccgc tggacgggga
    11521 tgggcacgtg tcactggaaa cagtgagtcc ggacaaagac tggtacgttc atcttgtcaa
    11581 atcccagtgt tggaccaggt cagattctgc actgctggaa ggtgcagagc tggtgaatcg
    11641 gattcctgct gaagatatga atgccttcat gatgaactcg gagttcaacc taagcctgct
    11701 agctccatgc ttaagcctag ggatgagtga aatttctggt ggccagaaga gtgccctttt
    11761 tgaagcagcc cgtgaggtga ctctggcccg tgtgagcggc accgtgcagc agctccctgc
    11821 tgtccatcat gtcttccagc ccgagctgcc tgcagaaccg gcggcctact ggagcaagtt
    11881 gaatgatctg tttggggatg ctgcactgta tcagtccctg cccactctgg cccgggccct
    11941 ggcacagtac ctggtggtgg tctccaaact gcccagtcat ttgcaccttc ctcctgagaa
    12001 agagaaggac attgtgaaat tcgtggtggc aacccttgag gccctgtcct ggcatttgat
    12061 ccatgagcag atcccgctga gtctggatct ccaggcaggg ctggactgct gctgcctggc
    12121 cctgcagctg cctggcctct ggagcgtggt ctcctccaca gagtttgtga cccacgcctg
    12181 ctccctcatc cactgtgtgc acttcatcct ggaggccgtt gcagtgcagc ctggagagca
    12241 gcttcttagt ccagaaagaa ggacaaatac cccaaaagcc atcagcgagg aggaggagga
    12301 agtagatcca aacacacaga atcctaagta tatcactgca gcctgtgaga tggtggcaga
    12361 aatggtggag tctctgcagt cggtgttggc cttgggtcat aaaaggaata gcggcgtgcc
    12421 ggcgtttctc acgccattgc tcaggaacat catcatcagc ctggcccgcc tgccccttgt
    12481 caacagctac acacgtgtgc ccccactggt gtggaagctt ggatggtcac ccaaaccggg
    12541 aggggatttt ggcacagcat tccctgagat ccccgtggag ttcctccagg aaaaggaagt
    12601 ctttaaggag ttcatctacc gcatcaacac actaggctgg accagtcgta ctcagtttga
    12661 agaaacttgg gccaccctcc ttggtgtcct ggtgacgcag cccctcgtga tggagcagga
    12721 ggagagccca ccagaagaag acacagagag gacccagatc aacgtcctgg ccgtgcaggc
    12781 catcacctca ctggtgctca gtgcaatgac tgtgcctgtg gccggcaacc cagctgtaag
    12841 ctgcttggag cagcagcccc ggaacaagcc tctgaaagct ctcgacacca ggtttgggag
    12901 gaagctgagc attatcagag ggattgtgga gcaagagatt caagcaatgg tttcaaagag
    12961 agagaatatt gccacccatc atttatatca ggcatgggat cctgtccctt ctctgtctcc
    13021 ggctactaca ggtgccctca tcagccacga gaagctgctg ctgcagatca accccgagcg
    13081 ggagctgggg agcatgagct acaaactcgg ccaggtgtcc atacactccg tgtggctagg
    13141 gaacagcatc acacccctga gggaggagga atgggacgag gaagaggagg aggaggccga
    13201 cgcccctgca ccttcgtcac cacccacgtc tccagtcaac tccaggaaac accgggctgg
    13261 agttgacatc cactcctgtt cgcagttttt gcttgagttg tacagccgct ggatcctgcc
    13321 gtccagctca gccaggagga ccccggccat cctgatcagt gaggtggtca gatcccttct
    13381 agtggtctca gacttgttca ccgagcgcaa ccagtttgag ctgatgtatg tgacgctgac
    13441 agaactgcga agggtgcacc cttcagaaga cgagatcctc gctcagtacc tggtgcctgc
    13501 cacctgcaag gcagctgccg tccttgggat ggacaaggcc gtggcggagc ctgtcagccg
    13561 cctgctggag agcacgctca ggagcagcca cctgcccagc agggttggag ccctgcacgg
    13621 catcctctat gtgctggagt gcgacctgct ggacgacact gccaagcagc tcatcccggt
    13681 catcagcgac tatctcctct ccaacctgaa agggatcgcc cactgcgtga acattcacag
    13741 ccagcagcac gtactggtca tgtgtgccac tgcgttttac ctcattgaga actatcctct
    13801 ggacgtaggg ccggaatttt cagcatcaat aatacagatg tgtggggtga tgctgtctgg
    13861 aagtgaggag tccaccccct ccatcattta ccactgtgcc ctcagaggcc tggagcgcct
    13921 cctgctctct gagcagctct cccgcctgga tgcagaatcg ctggtcaagc tgagtgtgga
    13981 cagagtgaac gtgcacagcc cgcaccgggc catggcggct ctgggcctga tgctcacctg
    14041 catgtacaca ggaaaggaga aagtcagtcc gggtagaact tcagacccta atcctgcagc
    14101 ccccgacagc gagtcagtga ttgttgctat ggagcgggta tctgttcttt ttgataggat
    14161 caggaaaggc tttccttgtg aagccagagt ggtggccagg atcctgcccc agtttctaga
    14221 cgacttcttc ccaccccagg acatcatgaa caaagtcatc ggagagtttc tgtccaacca
    14281 gcagccatac ccccagttca tggccaccgt ggtgtataag gtgtttcaga ctctgcacag
    14341 caccgggcag tcgtccatgg tccgggactg ggtcatgctg tccctctcca acttcacgca
    14401 gagggccccg gtcgccatgg ccacgtggag cctctcctgc ttctttgtca gcgcgtccac
    14461 cagcccgtgg gtcgcggcga tcctcccaca tgtcatcagc aggatgggca agctggagca
    14521 ggtggacgtg aaccttttct gcctggtcgc cacagacttc tacagacacc agatagagga
    14581 ggagctcgac cgcagggcct tccagtctgt gcttgaggtg gttgcagccc caggaagccc
    14641 atatcaccgg ctgctgactt gtttacgaaa tgtccacaag gtcaccacct gctaaaacct
    14701 ctacttccaa tcgcaccatc atcaccatca ccatcaccac catgattaca aggatgacga
    14761 cgataagtga ggcggccgcc aacttgatat caagcttatc gataatcaac ctctggatta
    14821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    14881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    14941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    15001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    15061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    15121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    15181 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    15241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    15301 ttcccgcggg cgcccccctc ctcacggcga gcgaagcttg tcgagaagta ctagaggatc
    15361 tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat
    15421 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    15481 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    15541 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    15601 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
    15661 cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
    15721 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
    15781 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
    15841 cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa
    15901 accgtcatct tcggctactt t
//
