LOCUS       sequence-227342-        5991 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Empty E. coli - B. burgdorferi shuttle vector, blasticidin S 
            resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2B
FEATURES             Location/Qualifiers
     source          1..5991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(66..82)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(75..97)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1775..2363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2264..2283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5840..5861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5876..5881,5882..5899,5900..5906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5914..5930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5919..5941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5938..5954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5938..5954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacgtcgcta gcggatcccg gggtaccgat cctctagagt cgacctgcag gcatgcaagc
       61 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcggccg ccctaggtta
      241 atcttcaata acatgtaaac cacgacgttt taaatcttct aatgaatcta acattcctct
      301 tattaattca acaggatgtc cttcccaatc ttcaacaaca ccaacaattc ttaaaggttc
      361 acaagttcta taagattgtg ttggattacc aggaaatttt ttatttgtaa gatttggatc
      421 atcttcaaat ggtcctgttg gttcaactat atatatataa ccacgacctt caagaccaga
      481 tagtgacata gcaagttcag ctccccaaac tgctggttcc attaaagctg aaaaataaat
      541 atgtttaaga attcgaccat cttcaaaatg agaaataaat cctgtagtta gtaaatcacc
      601 aattgctaaa ttagctttag ttccatgata aaatggtcct tgtacttgtt tataattatc
      661 atgagaaata ggaatccaat cttttaccat gacgttacct cctcatgatg tttaactttg
      721 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
      781 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
      841 agtcataaca agccatgcgg ccgtacccga gcttcaagga agatttccta ttaaggttga
      901 acttaagagc ttaagcatag acgatttgaa aaaaatttta aaacaaacaa aaaattcttt
      961 aataaaacaa tatgttgcga tgtttaaggt ttatgattta gatttaaagt ttagtgagga
     1021 agctatagat agaattgcag agcttacttt taatatgaat cttgagagtg aaaatcttgg
     1081 tgccagaaga cttcacggtg ttatggaaat agtgcttgca gatctttttt ttgaagtgcc
     1141 tggcagtaag ttgaaaaaat ttgaaataaa cttggactat gttaataaaa aaatacaaat
     1201 taacgaacaa aaagatttga attattatat aatttagtta aaagcaattt taaatgaggg
     1261 aggtttccat catatggcta aacctttaag tcaagaagaa agtactttaa ttgaaagagc
     1321 tactgctact attaatagta ttcctattag tgaagattat agtgttgcta gtgctgcttt
     1381 aagtagtgat ggtagaattt ttactggtgt taatgtttat cattttactg gtggtccttg
     1441 tgctgaatta gttgttttag gtactgccgc cgctgctgct gctggtaatc tcacttgtat
     1501 tgttgctatt ggtaatgaaa atagaggtat tttaagtcct tgtggtagat gtagacaagt
     1561 tttattagat ttacatcctg gtattaaagc tattgttaaa gatagtgatg gtcaacctac
     1621 tgctgttggt attagagaat tattacctag tggttatgtt tgggaaggtt aacaattcgt
     1681 tcaagccgag atcggcttac gcgtccaaaa tcccttaacg tgagttttcg ttccactgag
     1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1921 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2401 tttgctggcc ttttgctcac atgttctttc ctgcactaga tccccggcaa gttcatcctt
     2461 agggttttca tatctcttaa aaagtcaatt tctgcttgat taatttcttg tggattattg
     2521 tttttgcggt ttttcatttt ttatctcctt gagtattgaa ttttttctta tatataaaat
     2581 atagcaaaaa ctatttttgc caactttttt acaaaaaatt ttttattaaa acacttaggg
     2641 ctttgctaaa ttctctttta aaagaactta gtaaagccct atggatttaa gaactgatta
     2701 tacttacgta gtaaaaatac tacagatatt aatagtatag accatatcaa aatcataatc
     2761 aatttatatt tatctaaaat tgtggtccat acaaaataca aaaaaattag gtttttctaa
     2821 attctatttt aaaagaactt agtaacacct aatctctaaa ttacttacaa aattctaaaa
     2881 ttagattttt agttcttcat atttctttaa aagtttttta agaaaatctt tttgattttc
     2941 aaaaatttca tgcatcataa aactagtaaa tttggatttt tttttgtaaa aatcataact
     3001 ttcttgggtt ttaagttgaa atcttaatgg ttttattgga ttttgcctag atttttttaa
     3061 cgccggactt tctttatctt ttataaaatt ataagaattt ttaataccat tttctattaa
     3121 ataatcttct tttataagcc cctcttgcag tgcagttgct aatcttaaat acttatacgc
     3181 ttgagttttt gctacatcaa agtcttttat aaaatcaaga aaagttttat aaccatcaaa
     3241 tttataatat tgattatctt ttatttcttt taaaatttta atattttcca ccctgtggaa
     3301 tatttctttt ttgaaattgt catttaactt ttcctttagt gtcttgtaat aagaaatttt
     3361 actttcatta atcattaaat tttgttcaac ataagtatct atcttttttg ttaaaacttt
     3421 aacaacacgt tttttttcca taaaaatttt cctttatttt tgcaacattt tccttaattg
     3481 atataattgt tactatttta gattttttaa tatccactta agaattaatc cctcactaga
     3541 atatttttta ttatagaatt catatacatg tttttctaaa tctataaatt tatcaacaat
     3601 tgctttatta tagggtgtat ctatcttttc ttttcttaat aatcgtctca tattctttaa
     3661 ataacaacaa acactgccgg ttttaaacct aaattctatg taatatattt ttgaaaaagt
     3721 atatacattt tcaaccccgt ttatttcata ctttacaaaa acattattta ttggttttct
     3781 ataaccatag taaatgccta aaaatttgtc cccgtctttc ataggatata gataaagttt
     3841 ttgaatctct gatttatcta ataatttttc aaaaaaaatt atacaccgct tttgtttgtt
     3901 actaactact ctatgcaatg cctttaatat ttttgtgtga tatatatttt tatcaccttt
     3961 tttttctttc agcaggaaga tactttcttt tggaaattta attttttgtt catttattct
     4021 tgcagcctta agtccgattt tcatatttaa tccttatata gcctttctat ttaattcttt
     4081 taaactcaat aaactttttt ggtttttcat tagttctaat aattcgcaat aatatgtgca
     4141 aaacacttta ttgtattcta ttttcttttg gttattcaaa tattctttta taattggctt
     4201 tagaagttca atatttactt ctttttttag ttgttcaata aggatattga aaatgtttgt
     4261 ttttaaatct tgataatttt gttgagaatt ttcttttttt ctttcaattg acttctctaa
     4321 tttacgcttt atattgttta aatcgctata tttatgactt tcaataataa aatgaggctt
     4381 aaatttgtaa ttttcgtata ttttttggaa acttgtttct aattgtttag aattatattc
     4441 atttttttca aattcttttt gagtgttgca tagaatttcc tttaattttt cttgtttttc
     4501 tttcatacaa gatttagtaa aatgtatatt tttatttttt agtaatttaa tttctattat
     4561 ttttatgatt ttaagtattt tgattatatt atctttatca atatttaaat tgaataaaat
     4621 tgtaagaaaa attgaaagaa tttttttaca taaaaagtta catttattga agtaatttct
     4681 tacttgatac ttttctattt cattaatctt tctttcttct tttatattat ttttattatt
     4741 taaacactcc tttaaatcta cactcccatt tttagtaaat ctatctttaa ggccccttga
     4801 aactctattt tgaaatctta aatctttttt ttcttgaaag tatttataga ttttttggta
     4861 acattctttt tttgaaaaat taagttgata gtatatttcg gtccccaaat ttacccctaa
     4921 gtgtttgtga taattagttg tgactttaaa ttctttttct aatttgtaaa ggtaattttg
     4981 cagtgttttt agtttaacgg gtgtttgact atttctttta aggttttcat taaagtaata
     5041 gagtatgttt ttttgtgtgt atttcttata tgttttattt atgtacgcta gtgttgagat
     5101 aaggactact aatttatact gtaatttatt gtagcaagtt gtggttttac tggttttgtt
     5161 tatattagat aagttttcca tttatagctc cttgaatacc ggatttttta tctataaagt
     5221 atatatatca caattttgat ttaaaagtaa attctttttt taaaaataca aaaatttttt
     5281 ataaattata tagaccatga tattgtatta atactaattg attttaattt aaggttgaac
     5341 taaaataaac atagttttgt agaaaataat ttttcattat tttgctactt gatattggat
     5401 cgtaaaaata ttagggcttt acaaagttct tttaaaagag aatttagtaa agccctaagt
     5461 cttttaataa aaaatttttt gtaaaaaagt tggcaaaaat agtttttgct atatatttat
     5521 atataagaaa aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa caataatcca
     5581 caaaaaatta atcaaataaa taagcaaact aattttattg gttttgaaaa ttttatagaa
     5641 cttgataatc aagttaatca aactaaagaa aaaagtaaat taagtaagat aggtaagaaa
     5701 ttacccggaa taagcagtca agtctagtgc ggccgcgccc aatacgcaaa ccgcctctcc
     5761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5881 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5941 gaaacagcta tgacatgatt acgaattcga gctcggtaca ccggtctcga g
//
