LOCUS       sequence-227462-        5781 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin 
            PJ, Vogelstein B, Kinzler KW
  TITLE     Identification of c-MYC as a target of the APC pathway.
  JOURNAL   Science. 1998 Sep 4. 281(5382):1509-12.
  PUBMED    9727977
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Sep 4. 281(5382):1509-12."
COMMENT     Sequence Label: c-myc promoter (Del 4)
FEATURES             Location/Qualifiers
     source          1..5781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      993..1002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             999..2651
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1065..1083)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2692..2813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2703..2722)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2757..2776
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3113..3130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3284..3872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3364..3383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4043..4834,4835..4903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4666..4685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4904..5008)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5035..5490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5122..5141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5332..5353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5621..5669
                     /note="synthetic polyadenylation signal"
     misc_feature    5683..5774
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5723..5742
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacagc tgttccgcct gcgatgattt
       61 atactcacag gacaaggatg cggtttgtca aacagtactg ctacggagga gcagcagaga
      121 aagggagagg gtttgagagg gagcaaaaga aaatggtagg cgcgcgtagt taattcatgc
      181 ggctctctta ctctgtttac atcctagagc tagagtgctc ggctgcccgg ctgagtctcc
      241 tccccacctt ccccaccctc cccaccctcc ccataagcgc ccctcccggg ttcccaaagc
      301 agagggcgtg ggggaaaaga aaaaagatcc tctctcgcta atctccgccc accggccctt
      361 tataatgcga gggtctggac ggctgaggac ccccgagctg tgctgctcgc ggccgccacc
      421 gccgggcccc ggccgtccct ggctcccctc ctgcctcgag aagggcaggg cttctcagag
      481 gcttggcggg aaaaagaacg gagggaggga tcgcgctgag tataaaagcc ggttttcggg
      541 gctttatcta actcgctgta gtaattccag cgagaggcag agggagcgag cgggcggccg
      601 gctagggtgg aagagccggg cgagcagagc tgcgctgcgg gcgtcctggg aagggagatc
      661 cggagcgaat agggggcttc gcctctggcc cagccctccc gctgatcccc cagccagcgg
      721 tccgcaaccc ttgccgcatc cacgaaactt tgcccatagc agcgggcggg cactttgcac
      781 tggaacttac aacacccgag caaggacgcg actctcccga cgcggggagg ctattctgcc
      841 catttgggga cacttccccg ccgctgccag gacccgcttc tctgaaaggc tctccttgca
      901 gctggacgtc gatatcgaat tcgggtatat aatggatccg gtatcgagat ctgcgatcta
      961 agtaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa
     1021 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     1081 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     1141 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     1201 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     1261 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     1321 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     1381 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     1441 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     1501 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     1561 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     1621 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     1681 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     1741 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     1801 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     1861 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     1921 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     1981 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     2041 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     2101 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     2161 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     2221 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     2281 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     2341 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     2401 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     2461 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     2521 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     2581 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     2641 tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     2701 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2761 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2821 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2881 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     2941 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     3001 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ttatgactgt
     3061 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     5041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5341 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5401 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5461 attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt
     5521 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag
     5581 taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc
     5641 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
     5701 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
     5761 cagaacattt ctctatcgat a
//
