LOCUS       sequence-227703-        8916 bp    DNA     circular SYN 14-APR-2025
DEFINITION  ADAR1-p110 expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
  TITLE     Differential Binding of Three Major Human ADAR Isoforms to Coding 
            and Long Non-Coding Transcripts.
  JOURNAL   Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 
            10.3390/genes8020068.
  PUBMED    28208661
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2017-02-11"; volume: "8(2). pii: genes8020068. 
            doi"; pages: " 10.3390/genes8020068"
COMMENT     Sequence Label: pmGFP-ADAR1-p110
FEATURES             Location/Qualifiers
     source          1..8916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     493..514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3399..3416)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3405..3629
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3675..4103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3762..3781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3972..3993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4112..4132)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4117..4446
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4297..4432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4359..4378
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4513..5307
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4567..4586)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5177..5196
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5481..5602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5518..5537)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5572..5591
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5651..5667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5651..5667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5664..5686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5675..5691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5699..5705,5706..5723,5724..5729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5744..5765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5882..5899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6053..6641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6133..6152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6812..7603,7604..7672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7435..7454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7673..7777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7852..7871)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8043..8422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8423..8626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8577..8597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8671..8690
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8671..8689
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8717..8746
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(8756..8758,8759..8761,8762..8916,1..556)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8756..8758,8759..8761,8762..8916,1..556)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8801..8822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
       61 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      121 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      181 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      241 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      301 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      361 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      421 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      481 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      541 ggacgagctg tacaagtccg gcggcaagct tatggccgag atcaaggaga aaatctgcga
      601 ctatctcttc aatgtgtctg actcctctgc cctgaatttg gctaaaaata ttggccttac
      661 caaggcccga gatataaatg ctgtgctaat tgacatggaa aggcaggggg atgtctatag
      721 acaagggaca acccctccca tatggcattt gacagacaag aagcgagaga ggatgcaaat
      781 caagagaaat acgaacagtg ttcctgaaac cgctccagct gcaatccctg agaccaaaag
      841 aaacgcagag ttcctcacct gtaatatacc cacatcaaat gcctcaaata acatggtaac
      901 cacagaaaaa gtggagaatg ggcaggaacc tgtcataaag ttagaaaaca ggcaagaggc
      961 cagaccagaa ccagcaagac tgaaaccacc tgttcattac aatggcccct caaaagcagg
     1021 gtatgttgac tttgaaaatg gccagtgggc cacagatgac atcccagatg acttgaatag
     1081 tatccgcgca gcaccaggtg agtttcgagc catcatggag atgccctcct tctacagtca
     1141 tggcttgcca cggtgttcac cctacaagaa actgacagag tgccagctga agaaccccat
     1201 cagcgggctg ttagaatatg cccagttcgc tagtcaaacc tgtgagttca acatgataga
     1261 gcagagtgga ccaccccatg aacctcgatt taaattccag gttgtcatca atggccgaga
     1321 gtttccccca gctgaagctg gaagcaagaa agtggccaag caggatgcag ctatgaaagc
     1381 catgacaatt ctgctagagg aagccaaagc caaggacagt ggaaaatcag aagaatcatc
     1441 ccactattcc acagagaaag aatcagagaa gactgcagag tcccagaccc ccaccccttc
     1501 agccacatcc ttcttttctg ggaagagccc cgtcaccaca ctgcttgagt gtatgcacaa
     1561 attggggaac tcctgcgaat tccgtctcct gtccaaagaa ggccctgccc atgaacccaa
     1621 gttccaatac tgtgttgcag tgggagccca aactttcccc agtgtgagtg ctcccagcaa
     1681 gaaagtggca aagcagatgg ccgcagagga agccatgaag gccctgcatg gggaggcgac
     1741 caactccatg gcttctgata accagcctga aggtatgatc tcagagtcac ttgataactt
     1801 ggaatccatg atgcccaaca aggtcaggaa gattggcgag ctcgtgagat acctgaacac
     1861 caaccctgtg ggtggccttt tggagtacgc ccgctcccat ggctttgctg ctgaattcaa
     1921 gttggtcgac cagtccggac ctcctcacga gcccaagttc gtttaccaag caaaagttgg
     1981 gggtcgctgg ttcccagccg tctgcgcaca cagcaagaag caaggcaagc aggaagcagc
     2041 agatgcggct ctccgtgtct tgattgggga gaacgagaag gcagaacgca tgggtttcac
     2101 agaggtaacc ccagtgacag gggccagtct cagaagaact atgctcctcc tctcaaggtc
     2161 cccagaagca cagccaaaga cactccctct cactggcagc accttccatg accagatagc
     2221 catgctgagc caccggtgct tcaacactct gactaacagc ttccagccct ccttgctcgg
     2281 ccgcaagatt ctggccgcca tcattatgaa aaaagactct gaggacatgg gtgtcgtcgt
     2341 cagcttggga acagggaatc gctgtgtaaa aggagattct ctcagcctaa aaggagaaac
     2401 tgtcaatgac tgccatgcag aaataatctc ccggagaggt ttcatcaggt ttctctacag
     2461 tgagttaatg aaatacaact cccagactgc gaaggatagt atatttgaac ctgctaaggg
     2521 aggagaaaag ctccaaataa aaaagactgt gtcattccat ctgtatatca gcactgctcc
     2581 gtgtggagat ggcgccctct ttgacaagtc ctgcagcgac cgtgctatgg aaagcacaga
     2641 atcccgccac taccctgtct tcgagaatcc caaacaagga aagctccgca ccaaggtgga
     2701 gaacggagaa ggcacaatcc ctgtggaatc cagtgacatt gtgcctacgt gggatggcat
     2761 tcggctcggg gagagactcc gtaccatgtc ctgtagtgac aaaatcctac gctggaacgt
     2821 gctgggcctg caaggggcac tgttgaccca cttcctgcag cccatttatc tcaaatctgt
     2881 cacattgggt taccttttca gccaagggca tctgacccgt gctatttgct gtcgtgtgac
     2941 aagagatggg agtgcatttg aggatggact acgacatccc tttattgtca accaccccaa
     3001 ggttggcaga gtcagcatat atgattccaa aaggcaatcc gggaagacta aggagacaag
     3061 cgtcaactgg tgtctggctg atggctatga cctggagatc ctggacggta ccagaggcac
     3121 tgtggatggg ccacggaatg aattgtcccg ggtctccaaa aagaacattt ttcttctatt
     3181 taagaagctc tgctccttcc gttaccgcag ggatctactg agactctcct atggtgaggc
     3241 caagaaagct gcccgtgact acgagacggc caagaactac ttcaaaaaag gcctgaagga
     3301 tatgggctat gggaactgga ttagcaaacc ccaggaggaa aagaactttt atctctgccc
     3361 agtatagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3421 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3481 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3541 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3601 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3661 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3721 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3781 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3841 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3901 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3961 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4021 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4081 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4201 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4261 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4321 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4381 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4441 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4501 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4561 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4621 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4681 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4741 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4801 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4861 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4921 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4981 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5041 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5101 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5161 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5221 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5281 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5341 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5401 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5461 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5581 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6781 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6841 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6901 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6961 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7021 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7081 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7141 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7201 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7261 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7321 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7381 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7441 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7501 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7561 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7621 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7681 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7741 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7801 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     7861 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     7921 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     7981 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     8041 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     8101 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     8161 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     8221 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     8281 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     8341 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     8401 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     8461 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     8521 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     8581 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     8641 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     8701 tggctagccc accatggaac aaaaactcat ctcagaagag gatctgtccg gcggcatggt
     8761 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     8821 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     8881 gctgaccctg aagttcatct gcaccaccgg caagct
//
