LOCUS       sequence-22779-e         736 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Hedgepeth CM, Deardorff MA, Rankin K, Klein PS
  TITLE     Regulation of glycogen synthase kinase 3beta and downstream Wnt 
            signaling by axin.
  JOURNAL   Mol Cell Biol. 1999 Oct . 19(10):7147-57.
  PUBMED    10490650
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Oct . 19(10):7147-57."
COMMENT     Sequence Label: Axin GID 5-6 LP/pCS2MT
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..59
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             69..98
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             108..137
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             147..176
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             186..215
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    364..498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(388..407)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     442..461
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(611..631)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(611..629)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(650..666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(650..666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(663..685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    674..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(697..703,704..721,722..726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gtctttgcgg accatcgatt taagctatgg agcaaaagct catttctgaa gaggacttga
       61 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
      121 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
      181 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagagc ttgggcgacc
      241 tcaccatgga gattcacgtc gatcctgaaa aatttgctgc tgagcctata agtcgcttgg
      301 agggggtcct gagagaccgt gaagcactcg agcctctaga actatagtga gtcgtattac
      361 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      421 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      481 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      541 gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca
      601 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
      661 ttcctgtgtg aaattgttat ccgctcacaa ttcccacaac atacgagccg ggagcataaa
      721 gtgtaagcnt gggtgc
//
