LOCUS       sequence-22781-e         921 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Hedgepeth CM, Deardorff MA, Rankin K, Klein PS
  TITLE     Regulation of glycogen synthase kinase 3beta and downstream Wnt 
            signaling by axin.
  JOURNAL   Mol Cell Biol. 1999 Oct . 19(10):7147-57.
  PUBMED    10490650
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Oct . 19(10):7147-57."
COMMENT     Sequence Label: Axin GID 1-2/pET29b
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(380..397)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(413..457)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             468..490
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    505..529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(529..548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(530..548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(636..655)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtccaga ggagaacccc
       61 catcatcccc ttcttcttca gctcggacgc gtttaagtcg ctcttcaagc ttctgttctg
      121 cttcacggtc tctcaggacc ccctccaagc gacttataag ctcagcagca aatttttcag
      181 gatcgacgtg aatatccttt ggcatgtgat atgtacgagg aatgtgaggt agcggtccac
      241 gaccatttgc gttggcactc tcttgcattt ctctgcgata gtgttttcgg agtctgtacg
      301 gtgggatccc atctacgctg ctgtcagtta atgacatggt atcggcatcg ctggacatac
      361 tctgttgctc gctctccatg gaaccgcgtg gcaccagggt acccagatct gggctgtcca
      421 tgtgctggcg ttcgaattta gcagcagcgg tttctttcat atgtatatct ccttcttaaa
      481 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      541 tcgtattaat ttcgcgggat cgagatcgat ctcgatcctc tacgccggac gcatcgtggc
      601 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      661 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      721 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      781 ggcggtgctc aacggcctca cctactactg ggctgcttcc taatgcagga gtcgcataag
      841 gagagcgtcg agatcccgga caccatcgat gcgcaaacct ttcgcggtat ggcatgatag
      901 cgcccggaag agagtcattc a
//
