LOCUS       sequence-227900-        5349 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor Vector containing ZNF484 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF484
FEATURES             Location/Qualifiers
     source          1..5349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2668..2767)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2784..2803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2785..2803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2808..2824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2808..2824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3255..4046
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4139..4727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4628..4647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4881..4898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5057..5084)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5176..5262)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5311..5333
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5325..5342
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5326..5342
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 atgagcaatg cttttttata atgccaactt tgtacaaaaa agttggcacc atgaccaagt
      121 ccctggaatc agtgtcattc aaggacgtaa ctgtagactt cagtagggat gagtggcaac
      181 aattagacct tgctcagaaa agcctgtaca gagaagtgat gctggaaaac tatttcaact
      241 tgatctcagt gggatgtcaa gttcccaaac cagaagtcat cttcagcttg gaacaagaag
      301 agccatgtat gttggatggt gagatcccca gtcagagccg tccagatggg gacattggtt
      361 ttggaccttt acaacagagg atgtctgaag aagtttcttt ccagtctgag attaatatta
      421 atctcttcac aagagatgac ccatattcca ttttagaaga attgtggaaa gacgatgaac
      481 acacaagaaa atgtggagaa aaccagaaca aacctttaag tcgtgttgtc ttcattaaca
      541 agaaaacact agctaatgac agcatctttg aatataagga cattggggaa atagttcatg
      601 taaacacaca cctggtttcc tcaagaaaaa gaccccataa ctgtaactcg tgtggaaaga
      661 atttggagcc tatcataacc ttatataata gaaacaatgc aacagaaaat tctgataaga
      721 ctattggaga tggtgatatt ttcactcatt tgaattctca tacagaagtg actgcttgtg
      781 aatgtaacca atgtgggaaa cctctgcatc ataagcaagc tctcattcaa caacagaaaa
      841 ttcatactag agagagcctc tatttgtttt ctgactacgt aaatgttttc tccccgaagt
      901 cacatgcctt tgcacatgag agtatttgtg ctgaagaaaa gcagcatgaa tgccatgaat
      961 gtgaggcagt cttcactcag aagtcccagc ttgatggcag tcagagggtt tatgcaggaa
     1021 tatgcactga atatgagaag gatttttccc tcaagtcaaa ccgtcagaaa actccttatg
     1081 aggggaatta ctataaatgc agtgactatg gaagagcctt tatccagaag tcagatctgt
     1141 tcagatgcca gagaattcat tctggagaaa aaccttatga gtacagtgaa tgtgagaaaa
     1201 acctccctca gaattcaaac cttaatatac ataaaaaaat tcatactgga gggaaacact
     1261 ttgaatgtac tgaatgtgga aaagctttca caaggaaatc aacactaagt atgcatcaga
     1321 aaatccatac aggagaaaaa ccttatgtat gtactgaatg tgggaaggcc tttatccgga
     1381 agtcacattt tatcacacat gaaagaattc atactggaga aaaaccctat gaatgcagtg
     1441 actgtgggaa atcctttatt aaaaaatcac aactccatgt gcatcagcga attcacacag
     1501 gagagaatcc ctttatatgt tcagaatgtg ggaaggtctt cactcacaag acaaatctca
     1561 ttatacacca gaaaattcat acaggagaaa gaccctatat atgtactgtg tgtggtaagg
     1621 cctttactga caggtcaaat ctcattaagc accaaaaaat tcatactgga gagaaacctt
     1681 ataaatgcag cgactgtgga aaatcattca cctggaagtc tcggctcagg atacatcaga
     1741 agtgtcatac tggagagaga cattatgaat gcagtgaatg tgggaaagca ttcattcaga
     1801 agtcaacatt aagtatgcac cagagaattc atagagggga aaaaccatat gtttgcactg
     1861 aatgtggtaa ggccttcttc cacaaatccc attttattac acatgagaga attcatactg
     1921 gagagaaacc ctatgaatgc agtatttgtg ggaaatcctt cactaagaaa tcacagctcc
     1981 acgtacatca gcagattcac acaggagaga aaccctatag gtgtgctgaa tgtggaaagg
     2041 cttttactga cagatcaaat ctctttacac accagaaaat tcacactgga gagaaacctt
     2101 ataaatgtag tgactgtggg aaagccttca ctaggaagtc aggtctccat atacatcagc
     2161 aatcccatac tggagaaagg cattatgagt gcagtgaatg tgggaaagcc tttgcaagaa
     2221 aatcaacact aattatgcat cagagaattc atacaggaga gaaaccctat atttgtaatg
     2281 aatgtgggaa atccttcatc cagaagtcac acttaaatag acacaggaga attcatactg
     2341 gagagaaacc ctatgaatgc agtgactgtg gcaagtcttt cattaagaaa tcacaactcc
     2401 atgagcatca tcgaattcac acaggagaga aaccatatat atgtgctgag tgtggaaagg
     2461 ccttcaccat cagatcaaat cttattaaac accagaaaat tcatactaaa cagaaaccct
     2521 ataagtgcag tgacttgggg aaagccttaa actggaagcc acaactcagt atgcctcaga
     2581 aatctgacaa tggggaagta gagtgctcca tgccacaatt atggtgtggg gactcagaag
     2641 gtgaccaagg ccaactttct tctatcttgc caactttctt gtacaaagtt ggcattataa
     2701 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2761 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2821 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2881 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2941 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     3001 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3061 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3121 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3181 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3241 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3301 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3361 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3421 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3481 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3541 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3601 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3661 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3721 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3781 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3841 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3901 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3961 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     4021 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     4081 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4201 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4261 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4741 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4801 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4861 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4921 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4981 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     5041 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     5101 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     5161 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     5221 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5281 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     5341 gtcttaagc
//
