LOCUS       sequence-228088-        9542 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 1 Golden Gate Cassette: AsCas12a expression cassette for 
            dicotyledonous plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR1CB0014
FEATURES             Location/Qualifiers
     source          1..9542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..783
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             874..4794
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4514..4523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4795..4842
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4849..4929
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      4945..5197
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5245..5269
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5425..6573)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6909..7497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6989..7008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7668..8459,8460..8528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8291..8310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8529..8633)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8720..9351
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9443..9467
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac
      841 aacattacaa ttacatttac aattatcgat acaatgacac agttcgaggg ctttaccaac
      901 ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag
      961 cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag
     1021 ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg
     1081 cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag
     1141 gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac
     1201 ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac
     1261 aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg
     1321 accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc
     1381 tccggctttt atgagaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc
     1441 ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca
     1501 cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc
     1561 ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg
     1621 ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca
     1681 ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat
     1741 gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc
     1801 ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg
     1861 atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc
     1921 gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag
     1981 aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg
     2041 tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg
     2101 cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag
     2161 gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc
     2221 ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct
     2281 cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc
     2341 aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct
     2401 tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag
     2461 aagttcaagc tgaactttca gatgcctaca ctggcctctg gctgggacgt gaataaggag
     2521 aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca
     2581 aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc
     2641 tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc
     2701 acccagctga aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc
     2761 aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag
     2821 aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac
     2881 agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag
     2941 acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag
     3001 tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag
     3061 gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac
     3121 tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt
     3181 tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac
     3241 cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag
     3301 aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat
     3361 gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg
     3421 atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc
     3481 tttttccacg tgcctatcac actgaactat caggccgcca attccccatc taagttcaac
     3541 cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgatcgg
     3601 ggcgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag
     3661 cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag
     3721 gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag
     3781 ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg
     3841 gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag
     3901 gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag
     3961 gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc
     4021 acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat
     4081 acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag
     4141 aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa
     4201 accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg
     4261 cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc
     4321 aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc
     4381 accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag
     4441 ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct
     4501 cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat
     4561 gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc
     4621 gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac
     4681 atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg
     4741 cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg caacaaaagg
     4801 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat
     4861 gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc
     4921 gactatgcct aagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
     4981 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     5041 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     5101 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     5161 aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctactattg tcttctgcac
     5221 gaagtggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     5281 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     5341 atttgtatgt gcatgccaac cacagggttc cccagatcag gcgctggctg ctgaaccccc
     5401 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     5461 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
     5521 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
     5581 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
     5641 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
     5701 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
     5761 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
     5821 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
     5881 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
     5941 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
     6001 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
     6061 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
     6121 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
     6181 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
     6241 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
     6301 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcaaga cgacgcgaac gctccacggc
     6361 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
     6421 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
     6481 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
     6541 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
     6601 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
     6661 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
     6721 cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc
     6781 gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt
     6841 gtcgccggca tcgttgcgcc acatctagga tctgccagga accgtaaaaa ggccgcgttg
     6901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7801 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8521 atactcatac tcttcctttt tcaatattat tgaagcattt atcaaggtta ttgtctcatg
     8581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacgaat
     8641 tggccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
     8701 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
     8761 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
     8821 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
     8881 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
     8941 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
     9001 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
     9061 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
     9121 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
     9181 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
     9241 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
     9301 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cgcaaaggag
     9361 atcctgatct gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     9421 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     9481 gacaacttaa taacacattg cggacgtttt taatgtactg gggtggatgc agtgggcccc
     9541 ac
//
