LOCUS       sequence-228670-        1437 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Barcoded lentiviral vector to express ERG in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ERG_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccagca ctattaagga agccttatca gttgtgagtg aggaccagtc gttgtttgag
       61 tgtgcctacg gaacgccaca cctggctaag acagagatga ccgcgtcctc ctccagcgac
      121 tatggacaga cttccaagat gagcccacgc gtccctcagc aggattggct gtctcaaccc
      181 ccagccaggg tcaccatcaa aatggaatgt aaccctagcc aggtgaatgg ctcaaggaac
      241 tctcctgatg aatgcagtgt ggccaaaggc gggaagatgg tgggcagccc agacaccgtt
      301 gggatgaact acggcagcta catggaggag aagcacatgc cacccccaaa catgaccacg
      361 aacgagcgca gagttatcgt gccagcagat cctacgctat ggagtacaga ccatgtgcgg
      421 cagtggctgg agtgggcggt gaaagaatat ggccttccag acgtcaacat cttgttattc
      481 cagaacatcg atgggaagga actgtgcaag atgaccaagg acgacttcca gaggctcacc
      541 cccagctaca atgccgacat ccttctctca catctccact acctcagaga gactcctctt
      601 ccacatttga cttcagatga tgttgataaa gccttacaaa actctccacg gttaatgcat
      661 gctagaaaca cagggggtgc agcttttatt ttcccaaata cttcagtata tcctgaagct
      721 acgcaaagaa ttacaactag gccagattta ccatatgagc cccccaggag atcagcctgg
      781 accggtcacg gccaccccac gccccagtcg aaagctgctc aaccatctcc ttccacagtg
      841 cccaaaactg aagaccagcg tcctcagtta gatccttatc agattcttgg accaacaagt
      901 agccgccttg caaatccagg cagtggccag atccagcttt ggcagttcct cctggagctc
      961 ctgtcggaca gctccaactc cagctgcatc acctgggaag gcaccaacgg ggagttcaag
     1021 atgacggatc ccgacgaggt ggcccggcgc tggggagagc ggaagagcaa acccaacatg
     1081 aactacgata agctcagccg cgccctccgt tactactatg acaagaacat catgaccaag
     1141 gtccatggga agcgctacgc ctacaagttc gacttccacg ggatcgccca ggccctccag
     1201 ccccaccccc cggagtcatc tctgtacaag tacccctcag acctcccgta catgggctcc
     1261 tatcacgccc acccacagaa gatgaacttt gtggcgcccc accctccagc cctccccgtg
     1321 acatcttcca gtttttttgc tgccccaaac ccatactgga attcaccaac tgggggtata
     1381 taccccaaca ctaggctccc caccagccat atgccttctc atctgggcac ttactac
//
