LOCUS       sequence-228694-        1005 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Barcoded lentiviral vector to express GATA1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_GATA1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagttcc ctggcctggg gtccctgggg acctcagagc ccctccccca gtttgtggat
       61 cctgctctgg tgtcctccac accagaatca ggggttttct tcccctctgg gcctgagggc
      121 ttggatgcag cagcttcctc cactgccccg agcacagcca ccgctgcagc tgcggcactg
      181 gcctactaca gggacgctga ggcctacaga cactccccag tctttcaggt gtacccattg
      241 ctcaactgta tggaggggat cccagggggc tcaccatatg ccggctgggc ctacggcaag
      301 acggggctct accctgcctc aactgtgtgt cccacccgcg aggactctcc tccccaggcc
      361 gtggaagatc tggatggaaa aggcagcacc agcttcctgg agactttgaa gacagagcgg
      421 ctgagcccag acctcctgac cctgggacct gcactgcctt catcactccc tgtccccaat
      481 agtgcttatg ggggccctga cttttccagt accttctttt ctcccaccgg gagccccctc
      541 aattcagcag cctattcctc tcccaagctt cgtggaactc tccccctgcc tccctgtgag
      601 gccagggagt gtgtgaactg cggagcaaca gccactccac tgtggcggag ggacaggaca
      661 ggccactacc tatgcaacgc ctgcggcctc tatcacaaga tgaatgggca gaacaggccc
      721 ctcatccggc ccaagaagcg cctgattgtc agtaaacggg caggtactca gtgcaccaac
      781 tgccagacga ccaccacgac actgtggcgg agaaatgcca gtggggatcc cgtgtgcaat
      841 gcctgcggcc tctactacaa gctacaccac cagcactact gtggtggctc cgctcagctc
      901 atgagggcac agagcatggc ctccagagga ggggtggtgt ccttctcctc ttgtagccag
      961 aattctggac aacccaagtc tctgggcccc aggcaccccc tggct
//
