LOCUS       sequence-228907-        1317 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Barcoded lentiviral vector to express MYC Thr58Ala in mammalian 
            cells under control of EF1a promoter. Barcoded for transcript 
            detection in single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYC Thr58Ala_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1317
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPAPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
ORIGIN
        1 atgcccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag
       61 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg
      121 cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc cgccccgccc
      181 ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc
      241 tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag
      301 atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac
      361 gacgagacct tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc
      421 gccgccaagc tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc
      481 agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat
      541 ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac
      601 gacagcagct cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg
      661 gattctctgc tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc
      721 catgaggaga caccgcccac caccagcagc gactctgagg aggaacaaga agatgaggaa
      781 gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc agagtctgga
      841 tcaccttctg ctggaggcca cagcaaacct cctcacagcc cactggtcct caagaggtgc
      901 cacgtctcca cacatcagca caactacgca gcgcctccct ccactcggaa ggactatcct
      961 gctgccaaga gggtcaagtt ggacagtgtc agagtcctga gacagatcag caacaaccga
     1021 aaatgcacca gccccaggtc ctcggacacc gaggagaatg tcaagaggcg aacacacaac
     1081 gtcttggagc gccagaggag gaacgagcta aaacggagct tttttgccct gcgtgaccag
     1141 atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca
     1201 gcatacatcc tgtccgtcca agcagaggag caaaagctca tttctgaaga ggacttgttg
     1261 cggaaacgac gagaacagtt gaaacacaaa cttgaacagc tacggaactc ttgtgcg
//
