LOCUS       sequence-229108-        2244 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RBS  RBSc42 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::RBSc42
FEATURES             Location/Qualifiers
     source          1..2244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..169)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(182..200)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        268..372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(373..441,442..1233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(591..610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1404..1992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1893..1912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2150..2244
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ccagtgtggc tcttcgccaa caggatacat ctgtaaagga ggtaacgatg atgtgaagag
       61 cgtaagacct ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      121 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcccgaaag
      181 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      241 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      301 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      361 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      421 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      481 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      541 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      601 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      661 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      721 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      781 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      841 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      901 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      961 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1021 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1081 gagcgtgggt cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1141 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1201 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2101 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgccttgg actcctgttg
     2161 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     2221 cgggcgtttt ttattggtga gaat
//
