LOCUS       sequence-229166-        9156 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_171460-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4320..4337
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4389..4444
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4443..4462)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6680..6698)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6766..6870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6871..6939,6940..7731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7089..7108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7902..8490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8391..8410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8644..8661
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8814..8819,8820..8837,8838..8844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8852..8868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8857..8879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8876..8892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8876..8892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgaacatcaa agaggtggac gtcgacggca actactacgt gcagtggggc gacaacctgg
     1261 ccctgacctg cgcccaggat acccccccat tcggcaaggc cagcatcgac tggtttctgc
     1321 ccagccagct gaaccggccc gtgggctatg gcaacaaggc ccacgtgtac cggcaggacc
     1381 agctggacag catggtgctg gtgctggtcg tgcggagcgt gaacaccgac gacagcggca
     1441 cctacacctg tcggaccggc agacaggaag gcaaccagtt catcgagatg ggccggaagt
     1501 ccgtgaacgt gatcgtgcgg aagtccatcg tggccaccga ctgccccagg gaccagtgga
     1561 tccctgtgtc cggcgagatc accaactgct ggggcaacgg caccgagtgc ggcgtgccaa
     1621 cagccaccgt gcggtgcatc atcgaggcct tccctgcccc caccatccac tggcggttca
     1681 agggcgtgca gctgaccacc ggcaccaagt acatcatcac caccttcggc atcaccatca
     1741 tcaaccccac caccgaggac tccggcatct acaccgtgat cgcccggcag cctcagcaga
     1801 ccgccgtgtt cgacctgcag atcagcgcct tcagccggcc cagaatcacc agcggcccta
     1861 gcatcgtgcg gacccacaac aacaccttcg tgtccggcag agaagcctac ctgcagtgcc
     1921 tggccagcgg ccagcctccc cctacaatcc actggtatca cgagcgggac ccccagaccg
     1981 agctgcagaa ggccaacccc aagaaattct ccgtgaacac caacttcaga gtgggcctgc
     2041 tgtggatcag cgaggtgtcc taccccgagg atagcggcag ctacatctgc agagccgtga
     2101 tccccgtgcc cgccacctac agcgatagca ccctggtcac caccacagag gcccagatcc
     2161 ccatcgaagt gaccctgccc cccaccctga tccccctgac caccatgcac agctacgtgg
     2221 aactgggcgg caccgccacc gtgcagtgta gagtgcgggc caccaccccc ctggaactgt
     2281 acttcaagcg gttcaacagc agcaccccct acatcagcgg cgtgcagccc ggcgacaagc
     2341 ggatcagagt gtggcgcgag ggcgacatga acgaccccct gaaccacgac ctgtttctga
     2401 ccatcgagaa caccaccatc gacgacacct ggaactacag ctgccacgcc gtgaaccagg
     2461 gcaacagcag ctggtggaat accaccatcc aggtcatcca gaccccccag atcctgctgc
     2521 acagcaccct gaacaccgat agcgagctga gcggcctgag attcggctgg cgctaccagg
     2581 gcaccaacct gacctgcatc agccacggca tgccccaccc cacctggacc tggtacagac
     2641 ggggcgagca gatcctgaac ggccagaacc tgaccttcgt gatcatcagc tacgactact
     2701 gggaccacag ccagagctgg ctgcagatca ccccctgcct gtacaccgag cacttcatct
     2761 acgacgacta cgtgtgcaag gccaccaaca tcaagggcac caatgagagc aaggtgtcct
     2821 tcagacgggc cagcgtgccc ggccagccca gactggaaag ctacagcgtg acccagagca
     2881 ccatctttct gaacgtgtcc ccccccatca acaccggcgg catgcctacc ctggcctacg
     2941 agttcaccta ccgggccttt ggcggccctg aggggtggta cggccctgtg acataccccc
     3001 tggacggcag cagaagcagc ggcaagccca agttcgagct gacaggcctg ctgggcgaca
     3061 ccaactacca gctgaagctg agcgcctgct ctatcgtggg cagaggcatc ccctacagct
     3121 tcagcaccac caccgccagc agcaccagac ccggccctgt ggaagtgatc cacagcgcca
     3181 caggcatcta cccctacggc cacgtggtca actggatccc ccctatgagc ggcggctccc
     3241 ccatcctggg ctacagaatc agagtcagaa gcgtggaaaa cgccctgaac ctgatcagca
     3301 tggtggaaaa catcagcccc agcagcagct ggaaggtgta cacccccagc ttcaacaacc
     3361 cctacatgga ctactaccac ctggcccccc tgaagcccga ccagacatac cagctgatcg
     3421 tggaagccta caaccggcac ggctacagcc tggacggcgt ggacatcgag cagtacggct
     3481 acctgaaccg gacccccacc agcacccccc agctgcagca gatcttcccc ggcgagcggc
     3541 tgaactacca gaacagcgac aagctgagca acagcgtgaa cagcctgatg aacttcaagc
     3601 agagcgacct ggtgcccatc tggtatctgt tcgccacccc cagcgccgac gagctggacc
     3661 ctccttctgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     3721 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     3781 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     3841 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     3901 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     3961 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     4021 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     4081 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     4141 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     4201 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     4261 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     4321 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     4381 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     4441 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     4501 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     4561 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     4621 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     4681 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     4741 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     4801 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     4861 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     4921 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     4981 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     5041 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     5101 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     5161 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     5221 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     5281 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     5341 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     5401 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     5461 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     5521 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     5581 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     5641 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     5701 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     5761 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     5821 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     5881 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     5941 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     6001 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     6061 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     6121 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6181 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     6241 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     6301 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     6361 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     6421 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6481 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     6541 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6601 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     6661 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6721 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7081 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7381 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     7441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8881 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     8941 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     9001 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     9061 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     9121 tgtacattta tattggctca tgtccaatat gaccgc
//
