LOCUS       sequence-229240-        7410 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_061310-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2574..2591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2643..2698
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2697..2716)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4934..4952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5020..5124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5125..5193,5194..5985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5343..5362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6156..6744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6645..6664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6898..6915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7032..7053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7068..7073,7074..7091,7092..7098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7106..7122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7111..7133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7130..7146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7130..7146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 atgtgatcga gggcaccgtg gtcatgcccc ccgaggcccc tccagactgg cacgtgaaca
     1261 cccggatcaa tatcgccggc gaccagttcg tgggcttcgt gaaggccgac ggcaacttcg
     1321 aagtgaccaa cgtgcccagc ggcagctaca tcgtggaagt gatcaacccc gtgtactatt
     1381 ttcagggcgt gcgggtggac atcagcagca agggccggat ccgggccaga cagctgaacg
     1441 cccctcagcc caacgccgtg aaagagctgc cctaccccct gcggctgagc agcagcggca
     1501 gagccgtgta cttcaaggcc agagagcagc tgcggaccct ggacctgctg ttcaacccca
     1561 acgtgctgta cgtgctggtg cccctgctgc tggtggtcat cctgaccaag ctggtcaaca
     1621 ccaacgaccc cagcgtgcag aaagaaatgc agcagatgaa catcctgaac ccccagcagc
     1681 agctgcccga tatgagcgag ttcctgagca gcctgagcct gttcggcggc aacaccaaaa
     1741 agaccaaccc caccaccatc aacaagagaa agagcgccga gaagcaccgg caggtcacaa
     1801 acagcgccgg catcagcgag agccccagcc agtacaccac cgtgtccaac accagcaaca
     1861 ccaccagctc cagcagcaac agcgccagca agcggaccac caccaaggcc cggaaggccg
     1921 aaggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct
     1981 ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga
     2041 aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc
     2101 agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga
     2161 taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag
     2221 ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca
     2281 ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg
     2341 agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag
     2401 caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc
     2461 aggttttatc caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga
     2521 tgctgtggaa ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc
     2581 atcaccacca ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     2641 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2701 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     2761 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     2821 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     2881 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     2941 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     3001 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     3061 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     3121 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     3181 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     3241 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     3301 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     3361 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     3421 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     3481 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     3541 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     3601 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     3661 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     3721 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     3781 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     3841 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     3901 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     3961 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     4021 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     4081 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     4141 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     4201 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     4261 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     4321 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     4381 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4441 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4501 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4561 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     4621 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     4681 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4741 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     4801 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4861 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     4921 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4981 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5221 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5281 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5341 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5461 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5521 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5581 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5641 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5701 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5821 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5881 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5941 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6301 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6601 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6661 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6721 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6781 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6841 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6901 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6961 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7021 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7081 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7141 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     7201 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     7261 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
     7321 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     7381 tttatattgg ctcatgtcca atatgaccgc
//
