LOCUS       sequence-229301-        6515 bp    DNA     circular SYN 14-APR-2025
DEFINITION  human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Josefsson SE, Beiske K, Blaker YN, Forsund MS, Holte H, Ostenstad B,
            Kimby E, Koksal H, Walchli S, Bai B, Smeland EB, Levy R, Kolstad A, 
            Huse K, Myklebust JH
  TITLE     TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector 
            Function in B-cell Non-Hodgkin Lymphoma.
  JOURNAL   Cancer Immunol Res. 2019 Mar;7(3):355-362. doi: 
            10.1158/2326-6066.CIR-18-0351. Epub 2019 Jan 18.
  PUBMED    30659053
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2326-6066.CIR-18-0351"; journalName: "Cancer Immunol Res"; 
            date: "2019-03"; volume: "7"; issue: "3"; pages: "355-362"
COMMENT     Sequence Label: pMP71-hPDL-1
FEATURES             Location/Qualifiers
     source          1..6515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     671..690
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        671..689
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    721..745
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1661..1685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1930..2333
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             2839..3429
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3454..3470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3454..3470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3467..3489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3478..3494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3502..3508,3509..3526,3527..3532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3547..3568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3685..3702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3856..4444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4615..5406,5407..5475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5238..5257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5476..5580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5648..5666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5704..5726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5826..5845
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6039..6061
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6053..6070
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6054..6070
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
       61 tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac ctcgggggtc
      121 tttcatttgg aggttccacc gagatttgga gacccctgcc cagggaccac cgaccccccc
      181 gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg tgcgtgtttg
      241 tgccggcatc taatgtttgc gcctgcgtct gtactagttg gctaactaga tctgtatctg
      301 gcggtcccgc ggaagaactg acgagttcgt attcccggcc gcagcccctg ggagacgtcc
      361 cagcggcctc gggggcccgt tttgtggccc attctgtatc agttaaccta cccgagtcgg
      421 actttttgga gctccgccac tgtccgaggg gtacgtggct ttgttggggg acgagagaca
      481 gagacacttc ccgcccccgt ctgaattttt gctttcggtt ttacgccgaa accgcgccgc
      541 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
      601 gtctgaaaat tagctcgaca aagttaagta atagtccctc tctccaagct cacttacagg
      661 cggcctcgag taatacgact cactataggg agacccaagc tggctaggta agcttgatca
      721 acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatgag gatatttgct
      781 gtctttatat tcatgaccta ctggcatttg ctgaacgcat ttactgtcac ggttcccaag
      841 gacctatatg tggtagagta tggtagcaat atgacaattg aatgcaaatt cccagtagaa
      901 aaacaattag acctggctgc actaattgtc tattgggaaa tggaggataa gaacattatt
      961 caatttgtgc atggagagga agacctgaag gttcagcata gtagctacag acagagggcc
     1021 cggctgttga aggaccagct ctccctggga aatgctgcac ttcagatcac agatgtgaaa
     1081 ttgcaggatg caggggtgta ccgctgcatg atcagctatg gtggtgccga ctacaagcga
     1141 attactgtga aagtcaatgc cccatacaac aaaatcaacc aaagaatttt ggttgtggat
     1201 ccagtcacct ctgaacatga actgacatgt caggctgagg gctaccccaa ggccgaagtc
     1261 atctggacaa gcagtgacca tcaagtcctg agtggtaaga ccaccaccac caattccaag
     1321 agagaggaga agcttttcaa tgtgaccagc acactgagaa tcaacacaac aactaatgag
     1381 attttctact gcacttttag gagattagat cctgaggaaa accatacagc tgaattggtc
     1441 atcccagaac tacctctggc acatcctcca aatgaaagga ctcacttggt aattctggga
     1501 gccatcttat tatgccttgg tgtagcactg acattcatct tccgtttaag aaaagggaga
     1561 atgatggatg tgaaaaaatg tggcatccaa gatacaaact caaagaagca aagtgataca
     1621 catttggagg agacgtaact cgagaagggt gggcgcgccg acccagcttt cttgtacaaa
     1681 gtggttgatc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tcaattcgag
     1741 catcttaccg ccatttattc ccatatttgt tctgtttttc ttgatttggg tatacattta
     1801 aatgttaata aaacaaaatg gtggggcaat catttacatt ttatgggata tgtaattact
     1861 agttcaggtg tattgccaca agacaaacat gttaagaaac tttcccgtta tttacgctct
     1921 gttcctgtta atcaacctct ggattacaaa atttgtgaaa gattgactga tattcttaac
     1981 tatgttgctc cttttacgct gtgtggatat gctgctttaa tgcctctgta tcatgctatt
     2041 gcttcccgta cggctttcgt tttctcctcc ttgtataaat cctggttgct gtctctttat
     2101 gaggagttgt ggcccgttgt ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca
     2161 acccccactg gctggggcat tgccaccacc tgtcaactcc tttctgggac tttcgctttc
     2221 cccctcccga tcgccacggc agaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2281 gctaggttgc tgggcactga taattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     2341 tggctgctcg cctgtgttgc caactggatc ctgcgcggga cgtccttctg ctacgtccct
     2401 tcggctctca atccagcgga cctcccttcc cgaggccttc tgccggttct gcggcctctc
     2461 ccgcgtcttc gctttcggcc tccgacgagt cggatctccc tttgggccgc ctccccgcct
     2521 gtttcgcctc ggcgtccggt ccgtgttgct tggtcgtcac ctgtgcagaa ttgcgaacca
     2581 tggattccac cgtgaacttt gtctcctggc atgcaaatcg tcaacttggc atgccaagaa
     2641 taattcggat ccaagcttag gcctgctcgc tttcttgctg tcccatttct attaaaggtt
     2701 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
     2761 ggattctgcc tagcgctaag cttaacacga gccatagata gaataaaaga ttttatttag
     2821 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     2881 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     2941 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     3001 gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac aggatatctg
     3061 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     3121 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     3181 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     3241 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     3301 tagactgcgt cgcccgggta cccgtgttct caataaaccc tcttgcagtt gcatccgact
     3361 cgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactgccc acctcggggg
     3421 tctttcattc tcgagcagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3601 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttcgccctgg
     3961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4441 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4501 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4561 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5221 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5581 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5641 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5701 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5761 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5821 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5881 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     5941 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6001 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6061 gacggccagt gaattagtac tctagcttaa gtaacgccat tttgcaaggc atggaaaata
     6121 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
     6181 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagttgg
     6241 aacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     6301 gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca
     6361 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     6421 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     6481 acaacccctc actcggcgcg ccagtcctcc gatag
//
