LOCUS       sequence-229347-        1187 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Antibiotic resistance 
            marker for selection after transformation in Y. lipolytica 
            (Marker_Nat).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE368
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtctctagg ttagggataa cagggtaatg ataacttcgt atagcataca ttatacgaag
       61 ttatggggtt ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc caattgaccc
      121 caaattgacc cagtagcgga cccaaccccg gcgagagccc ccttcacccc acatatcaaa
      181 cctcccccgg ttcccacact tgccgttaag ggcgtagggt actgcagtct ggaatctacg
      241 cttgttcaga ctttgtacta gtttctttgt ctggccatcc gggtaaccca tgccggacgc
      301 aaaatagact actgaaaatt tttttgcttt gtggttggga ctttagccaa gggtataaaa
      361 gaccaccgtc cccgaattac ctttcctctt cttttctctc tctccttgtc aactcacacc
      421 cgaagcacaa tgaccactct ggatgacacc gcttaccgat accgaacttc cgttcctggc
      481 gatgccgagg ctattgaggc tctggatgga tctttcacca ctgacaccgt tttccgagtg
      541 accgctactg gcgacggctt caccctgcga gaggtgcctg tcgaccctcc tctcaccaag
      601 gttttccctg acgatgagtc ggacgatgag tctgacgctg gagaggacgg cgaccctgac
      661 tctcgaactt tcgtggctta cggcgacgat ggcgacctgg ccggctttgt ggtcgtttct
      721 tactccggat ggaaccgacg actgaccgtg gaggacatcg aggtcgctcc tgagcaccga
      781 ggtcatggtg tcggacgagc tctgatgggt ctggctactg agttcgctcg agagcgaggt
      841 gctggccacc tgtggctcga ggtcaccaac gttaacgccc ctgctattca tgcctaccga
      901 cgaatgggtt ttaccctgtg tggcctcgat actgccctgt acgacggaac cgcttccgat
      961 ggagagcagg ccctctacat gtcgatgccc tgcccttaag tgtctgtggt atctaagcta
     1021 tttatcactc tttacaactt ctacctcaac tatctacttt aataaatgaa tatcgtttat
     1081 tctctatgat tactgtatat gcgttcctct aagacaaatc gaatataact tcgtatagca
     1141 ggagttatcc gaagcgagta gggataacag ggtaatacgg agagacc
//
