LOCUS       sequence-230050-        1646 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer
            improved NahR (C75m) fused to superfolder GFP (C3m)). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1646)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C90m
FEATURES             Location/Qualifiers
     source          1..1646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             926..1639
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aatggaactg cgtgacctgg atttaaacct gctggtggtg ttcaaccagt tgctggtcga
       61 cagacgcgtc tctgtcactg cggagaacct gggcctgacc cagcctgccg tgagcaatgc
      121 gctgaaacgc ctgcgcacct cgctacagga cccactcttc gtgcgcacac atcagggaat
      181 ggaacccaca ccctatgccg cgcatctggc cgagcacgtc acttcggcca tgcacgcact
      241 gcgcaacgcc ctacagcacc atgaaagctt cgatccgctg accagcgagc gtaccttcac
      301 cctggccatg accgacattg gcgagatcta cttcatgccg cggctgatgg atgcgctggc
      361 tcaccaggcc cccaattgcg tgatcagtac ggtgcgcgac agttcgatga gcctgatgca
      421 ggccttgcag aacggaaccg tggacttggc cgtgggcctg cttcccaatc tgcaaactgg
      481 cttctttcag cgccggctgc tccagaatca ctacgtgtgc ctatgtcgca aggaccatcc
      541 agtcacccgc gaacccctga ctctggagcg cttctgttcc tacggccacg tgcgtgtcat
      601 cgccgctggc accggccacg gcgaggtgga cacgtacatg acacgggtcg gcatccggcg
      661 cgacatccgt ctggaagtgc cgcacttcgc cgccgttggc cacatcctcc agcgcaccga
      721 tctgctcgcc actgtgccga tatgtttagc cgactgctgc gtagagccct tcggcctaag
      781 cgccttgccg cacccagtcg tcttgcctga aatagccatc aacatgttct ggcatgcgaa
      841 gtaccacaag gacctagcca atatttggtt gcggcaactg atgtttgacc tgtttacgga
      901 tggaagcgga agtggatccg gtagtatgcg taaaggcgaa gagctgttca ctggtgtcgt
      961 ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga
     1021 gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa
     1081 actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc
     1141 tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta
     1201 tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt
     1261 gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga
     1321 agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat
     1381 caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga
     1441 ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc
     1501 tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa
     1561 cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg
     1621 tatggatgaa ctgtacaaat aaaggt
//
