LOCUS       sequence-230106-         167 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression 
            bi-cistronic RBS, engineered for context-independence, prefixed by 
            self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 167)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U24m
FEATURES             Location/Qualifiers
     source          1..167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        7..56
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             150..158
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tactagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
       61 tacaaataat tttgtttaag ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      121 attttcgtac tgaaacatct taatcatgct aaggaggttt tctaatg
//
