LOCUS       sequence-230389-       10763 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Yeast expression plasmid for PmCYP719A26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK
  TITLE     The biosynthetic origin of psychoactive kavalactones in kava.
  JOURNAL   Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0.
  PUBMED    31332312
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0."
COMMENT     Sequence Label: pJKW1148
FEATURES             Location/Qualifiers
     source          1..10763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      102..371
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(395..411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(408..430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(443..449,450..467,468..473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    488..509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(2259..2634)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(2827..2844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2998..3586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3078..3097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3757..4548,4549..4617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4380..4399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4618..4722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4790..4808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4846..4868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4968..4987
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5181..5203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5195..5212
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5196..5212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5777..7119
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6740..6787)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7500..7715
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7716..8519
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    8776..8893
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cgcgggggat ctcccatgtc tctactggtg gtggtgcttc tttggaatta ttggaaggta
       61 aggaattgcc aggtgttgct ttcttatccg aaaagaaata aattgaattg aattgaaatc
      121 gtagatcaat ttttttcttt tctctttccc catcctttac gctaaaataa tagtttattt
      181 tattttttga atatttttta tttatatacg tatatataga ctattattta tcttttaatg
      241 attattaaga tttttattaa aaaaaaattc gctcctcttt taatgccttt atgcagtttt
      301 tttttcccat tcgatatttc tatgttcggg ttcagcgtat tttaagttta ataactcgaa
      361 aattctgcgt tcgttaaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      541 cgggaaacct gtcgtgccag agcttcgtaa ccgacagttt ctaacttttg tgctttgaca
      601 agaacttctt cttcttgctt taataaaaac tgttccattt tcgttgtata acttgaatca
      661 taagcgccaa gcagtctgac agccaacagc gcagcgttcg tactattatt aatagcgacg
      721 gtagctactg gaacacctct aggcatttgc acaattgaat gtaaagaatc tactccatct
      781 agacaagaac cttttacggg cacaccgatg acaggaagtg gtgtcattgc agccaccata
      841 cctggcaagt gagcagcccc accagctcca gcgataattg ttttaattcc acgcttgctt
      901 gcggaaatag catatgctga catcctatgt ggagttctat gagcagagac tattgtcact
      961 tcaaatggaa cgccaaaatc ttttaaaacc gcacatgcgg cagacattac cggcaagtca
     1021 gagtctgatc ccatgatgat tccaaccaat ggtttgacca ttgcttccaa gtccaacttt
     1081 tgagcgacag agattttgat tggaatatca gttctacctg taatgtagtt cagcctttgt
     1141 tcacattccg ccatactgga ggcaataata tttatgtgac ctacttttct gttaggtcta
     1201 gactcttttc catataagta cactgaggaa cctggagtcg ccaatgctct ttcgcaagtt
     1261 tctagctctt tatcttttgt atgtttgtct ccaagaacat ttagcataat ggcgttcgtt
     1321 gtaatggtgg agaaagatgt gaaattcttt ggcattggca aatccaatat tgatctcaaa
     1381 tgagcttcaa attgagaagt gacgcaagca tcaatggtat aatgtccaga gttgtgaggc
     1441 cttggggcaa tttcgttaat aagcaattcc cctgtttcta aatagaacat ttccacacca
     1501 aatataccac aaccgggaaa agatttgatt gcattttctg ccaacaactt cgccttaagt
     1561 tgaacggagt ccggaactct agcaggcgca taacataagt cacaaatatt gtccttgtgg
     1621 atagtctcta caattgggta agaaaacact aaaccgttaa cagatctcac aatcatgact
     1681 gctaattctt tagtaaatgg tgcccatttt tcggcgtaca aaggacgatc cttcagtact
     1741 tccaaagctt ccggaatcat ttccttattc tttacaacga agttacctct tccatcgtat
     1801 gccaaagtcc tcgacttcaa gacgaatgga aaacccaaat ctcttccaac attcaatagg
     1861 gacgtctcac tggcttgttc cacaggaaca ctttgggtaa ctgctatacc atttttgatt
     1921 aaatgctctt tttgaatata tttgtcttgt atcaatctga ttgtttctgg agaagggtaa
     1981 atttttaatt tgggatgttt tacttgaaga ttctttagtg taggaacatc aacatgctca
     2041 atctcaatcg ttagcacatc acatttttca gctagttttt cgatatcaag aggattggaa
     2101 aaggagccat taacgtggtc attggagttg cttatttgtt tggcaggaga attttcagca
     2161 tctagtatta ccgtcttaat gttgagcctg tttgctgcct caacaatcat acgtcccaat
     2221 tgtccccctc ctaatatacc aactgttcta gaatccatac ttgattgttt tgtccgattt
     2281 tcttgttttt cttgattgtt atagtaggat gtacttagaa gagagatcca acgattttac
     2341 gcaccaattt atacatgaaa tgctccataa tattgtccat ttagttctta ataaaaggtc
     2401 agcaagagtc aatcacttag tattacccgg ttcgtagcca tgcaacaaga gtcatttgtc
     2461 agcatagctg taataatcaa tcatgacgta agaaatgtat cataattaaa agttgttaaa
     2521 gatgtcagtg ttatgttggt gttacaaaat tctcggcttc tcactaatat ttaatatctc
     2581 ttaaatttta tctgtctttg attcttttaa gaaaagttat gtattattca agaaaaagtc
     2641 aattccgcat caaaaggtaa aatttatata aactgcttta aaatttcatg aaactaggca
     2701 acttttcgaa atgatctttt tcgagcatga agtttctttt ataataacct ggtcaaaagc
     2761 tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3421 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3541 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3601 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3661 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3721 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3781 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3841 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3901 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3961 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4021 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4081 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4141 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4201 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4261 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4321 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4381 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4441 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4501 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4561 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4621 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4741 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4801 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4861 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4921 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     4981 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5041 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     5101 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5161 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat
     5221 tccacggact atagactata ctagtatact ccgtctactg tacgatacac ttccgctcag
     5281 gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca
     5341 aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa
     5401 gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac
     5461 gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca
     5521 aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa
     5581 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
     5641 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
     5701 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
     5761 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     5821 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     5881 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     5941 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     6001 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     6061 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     6121 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     6181 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     6241 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     6301 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     6361 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     6421 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     6481 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     6541 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     6601 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     6661 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     6721 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     6781 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     6841 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     6901 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     6961 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     7021 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     7081 ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac
     7141 tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac
     7201 ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt
     7261 cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat
     7321 agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt
     7381 atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg
     7441 ttttaggact ggttcagaat taattctcat gtttgacagc ttatcatcgc gataagcttt
     7501 tcaattcatc attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg
     7561 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
     7621 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
     7681 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
     7741 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
     7801 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
     7861 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
     7921 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
     7981 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
     8041 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
     8101 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
     8161 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
     8221 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
     8281 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
     8341 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
     8401 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
     8461 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
     8521 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
     8581 tatatcagtt attacccgat caaaaatcat cgcttcgctg attaattacc ccagaaataa
     8641 ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt tcatttgaag
     8701 gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt
     8761 agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac
     8821 cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca cccgctcggc
     8881 ggcttctaat ccgtacttca atatagcaat gagcagttaa gcgtattact gaaagttcca
     8941 aagagaaggt ttttttaggc taatcgacct cgagcagatc cgccaggcgt gtatatagcg
     9001 tggatggcca ggcaacttta gtgctgacac atacaggcat atatatatgt gtgcgacgac
     9061 acatgatcat atggcatgca tgtgctctgt atgtatataa aactcttgtt ttcttctttt
     9121 ctctaaatat tctttcctta tacattaggt cctttgtagc ataaattact atacttctat
     9181 agacacgcaa acacaaatac acacactaaa ttaccggatc catggagcaa gctcaatggg
     9241 tcgacccaac tctgctccct gcatttgtgg gcatcatctt cttcttcctt ggcatgttct
     9301 ttggaaggag ttctttggga gctgggaagg gtgcagcgcc tagaagcacc agttctaccg
     9361 agtggccaga cggccctcca aagctgccca tcatcggcaa cctgcaccag ctcaacaaag
     9421 gcggggagct ggtccaccac aacctcgcca agctcgccca gtcctacgac cgcgccatga
     9481 ccatctgggt cggcagctgg ggccccatga tcgtcgtcag cgacgccgac cttgcatggg
     9541 aggtcctcgt caccaagtcg ccggacttcg ccggccgggt gctctccaag ctctcgcact
     9601 tgttcaacgc caactacaac accgtcgtcg cctacgacgc cgggccgcaa tggcagtcgc
     9661 tccggcgagg tctgcagcac gggccgctcg gccccgccca tgtttctgcg caggctcgtt
     9721 tccacgaaga agacatgaag ctcctggtga gcgacatgat gagagcagca cagaaaggtg
     9781 gtagcaatgg agtggttgaa cctctggcct atgtccggcg agccactatc cgatttctgt
     9841 ctcgtctatg ctttggggag gccttcaacg acgaggcgtt cgtggagggg atggacgaag
     9901 cagtggagga gaccatcgga gccactggcc atgcacgcat cctcgacgcc ttctacttca
     9961 ctcgccacct ccctatcatc cgccgcagct tcatagatac cgtcaacgcc aagaagaaga
    10021 tcgagagcct tgtccggccg ttgctctccc ggccggcgcc accggggtct tacctccact
    10081 tcctcctttc caccgacgcg ccggagaata tgatcatctt tcgaatattc gaagtctact
    10141 tgctgggcgt ggacagcacc gcctccacca ccacatgggc tctcgccttc ctcgtctcca
    10201 accaacaggc gcaggagaag ctccacaatg agctcgccca gtactgcgcc agccagaaca
    10261 atcagatcat caaagcagac gacgtcggaa agctgtcgta cctgctcggg gtagtgaagg
    10321 agacgatgag gatgaagccg atagcgccgc tggccgtccc ccacaagacg ctcaaggaga
    10381 cgatgctcga cggaaagagg gtggcggcgg gaacgacggt ggtagtgaac ctctatgccg
    10441 tccactacaa cccgaagcta tggccggagc cggagcagtt ccgcccggag aggttcgtgg
    10501 tcggcgccag cggcggcaat ggtggggggt cttccgagta catgctgcag tcgtacctgc
    10561 cctttggagg ggggatgagg tcctgcgcag ggatggaggt gggaaagttg caggtggcga
    10621 tggtcgtggc caaccttgtg atggcattta aatggttgcc ggaggaggag gggaagatgc
    10681 cggacctggc tgaagacatg accttcgtgc tcatgatgaa gaagccattg gctgccaaaa
    10741 tcgttccacg tgcatgagaa ttc
//
