LOCUS       sequence-230422-        1277 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S293D into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, 
            Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA,
            Golshani A, Babu M
  TITLE     The conserved Tpk1 regulates non-homologous end joining 
            double-strand break repair by phosphorylation of Nej1, a homolog of 
            the human XLF.
  JOURNAL   Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 
            10.1093/nar/gkab585.
  PUBMED    34244791
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585."
COMMENT     Sequence Label: pLEX307_CR-PDHA2S293D
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             249..260
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggrctttcta tttmatgtcg tgaggctagc atgctggccg ccttcatctc ccgcgtgttg
       61 aggcgagttg cccagaaatc agctcgcaga gtgctggtgg catcccgtaa ctcctcaaat
      121 gacgctacat ttgaaattaa gaaatgtgat ctttatctgt tggaagaggg tccccctgtc
      181 actacagtgc tcactagggc ggaggggctt aaatactaca ggatgatgct gactgttcgc
      241 cgcatggaat tgaaggcaga tcagctgtac aaacagaaat tcattcgcgg tttctgtcac
      301 ctgtgygatg gtcaggaagc ttgttgcgtg ggccttgagg ccggcataaa tccctcggat
      361 cacgtcatta catcctatag ggctcatggt gtgtgctata ctcggggact ttctgtccga
      421 tccattctcg cagagctgac gggaagaaga ggaggttgtg ctaaaggaaa aggaggatcg
      481 atgcatatgt ataccaagaa cttctatggg ggcaatggca tcgtcggtgc acagggcccc
      541 ctgggcgctg gcattgctct ggcctgtaaa tataaaggaa acgatgagat ctgtttgact
      601 ttatatgggg atggcgctgc gaatcagggg cagatagccg aagctttcaa tatggcagct
      661 ttatggaaat taccttgtgt tttcatctgt gagaataacc tatatggaat gggaacatct
      721 actgagagag cagcagccag ccctgattac tacaagaggg gcaattttat ccctgggcta
      781 aaggtcgatg gaatggatgt tctgtgtgtt cgtgaggcaa caaaatttgc agctaactac
      841 tgtagatctg gaaaggggcc catactgatg gagctgcaaa cctaccgtta tcatggacac
      901 agtatggacg atcctggagt cagttatcgt acacgagaag aaattcacga agtaagaagt
      961 aagagggatc ctataataat tctccaagat agaatggtaa acagcaagct cgcactgtga
     1021 agattaagga aattggggct gagtgagaaa gaattgatga tgctgccagt tgctaccact
     1081 gatcctgagc acatttgaga tagccatcac atctacagca gtgatcatct tttgagtcgt
     1141 gtgcaatcca tgatcagtta gtcgtcagta actagtacgc gtagtcgaca tcactctgat
     1201 amaaattgga gatgactgga atcctacwtg ttgctcttta scttgtggga agcctgcctt
     1261 atakgccctt gtawttg
//
