LOCUS       sequence-230545-        4027 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GBPart - ELuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pELuc
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1829..1847)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1830..1847)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1863..1879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1863..1879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1994..2582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2074..2093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2697..2799
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2800..3459
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2866..2885)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3572..4000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3659..3678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3869..3890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaagctc aagccgccag
      121 ggcatagttc tcatcgttgg cggcggatgt cttgtacata gatccaccac ccgccttctc
      181 cataagtgct tctcggagtt cctttcggct gattttgccc gtgacgttcc tcggaataga
      241 gtcaacgaat ctgacacccc ctcgaagata cttgctgtgg ctaactcttt gtgcaaggaa
      301 atcatatact tctttagccg tcagttgggc cccaggttgc ttgacaacga atccggccgg
      361 aagttcccct gcttctatgt cggggatacc gactacggcc acatcgcgga tccctggatg
      421 ttggagcaaa atctcctcca actcgacagg agccacttga tatcccttgt acttgatcag
      481 ctctttatat ctatccacaa tgtaaaaata ttcatcctca tcatagtagc caaaatcacc
      541 actgtgaagc caaccatcgt cgtcaatggc ctccttggtg gcctgtggat tattcacgta
      601 gcctttcgtg accatggggc cccaaatgca caactctccc acttgatttg ggccaagcgc
      661 ctctccggtg tttcgatcta taattttagc ggccatgtag ggcgtcactt ttcccaatga
      721 accggattta aattcgttgt gaagagtgtg gatattagcg gatgtgcttt cagtcaaccc
      781 gtaaccacac cgtatgcctg gcaaattaag gcgttttacg gcgatttcag ctacttcttt
      841 tgcaagaggt gccgcaccac aacaaagttc cgccaaggta gaaagatcat acttatcaac
      901 caacggtgat ttagaaagaa agaggattgt acttgggaca ttgatcacac ttcgtacttc
      961 atagtcttga atcgccttca aaaaaacctc ttggttgaac ctccgaagca tcacgacgcg
     1021 cagccccacc ataaaatacc ccaagttaat ggaaaaccca aaggcgtgaa agaagggaag
     1081 atacgccagc acactaactc cgggaatgag ttgggtacca actctcgggt cagaggcgtg
     1141 ggtcagccga acacatatat tgcggtgggt ctgcatgacg cccttaggca acccggtagt
     1201 accactacta cacagtatcg ctgcaacctg gtcgaccgga tcgtaatgca gtggtttaaa
     1261 ggtctgaaga ttgttatcgc tatatcttga cataaaatta tgcaaggatt cacatccgag
     1321 aaggttttcc tcagagtcca gaattatgat tcttttgata taatttgttc tatccttaac
     1381 ctccaaaacc tttggaagta tctttcgcgt ggtgaacaca agtattggct tactaattcc
     1441 tgtgacctta cacagttcgt ctggtatata gtcctcgttt acaggtgcaa cgaccatgcc
     1501 tatataccac gcactaataa ttggaatgaa aaatcttttg ttgttttcag cgcatattga
     1561 tacaacatca ttcattttat agccacaatt ctggagactc tgccccaatt tgaccgtcgt
     1621 gtcgaagaat tcctgataac taagactttc gttacccatc acgtcgagga tcgcctgcgg
     1681 gatgtgacta tgtttgtgaa gcgcgttgta gagcatctct ccagcggtaa aattcccaag
     1741 cgggtgctta ggctcgggcc catacacaac attcttctct ctcttcatca ttcgagacca
     1801 ctcatcgccc aatcactagt gaattcgttc tatagtgtca cctaaatacc atagagccgt
     1861 tggtcatagc tgtttcctgt ccaggcatca tgacacggtt atccacagaa tcaggggata
     1921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgtgatgat
     2701 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     2761 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2821 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2881 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     2941 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     3001 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     3061 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     3121 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     3181 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtgtcagcc
     3241 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     3301 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     3361 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     3421 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     3481 tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga
     3541 aattcgatga taagctgtca aacatgatgt tacgcgccct gtagcggcgc attaagcgcg
     3601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3781 cttgattagg gtgatggttc acgtagtggg ccagcgccct gatagacggt ttttcgccct
     3841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4021 acaattt
//
