LOCUS       sequence-231093-        2858 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo golden gate assembly CD part for LuxRQ-FMY (Adam Meyer's 
            improved LuxR activator from https://doi.org/10.1101/285866, 
            selected for dynamic range and low cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C74m
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(154..945,946..1014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     777..796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1015..1119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1187..1205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1209..1252)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1255..1276
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             2040..2051
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2056..2076
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2082..2099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2253..2841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2333..2352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
       61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1201 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     1261 gcggccgctt ctagagacta gtgggtctca aatgaaaaac ataaatgccg acgacacata
     1321 cagaataatt aataaaatta aagcttttag aagcaataat gatattaatc aatgcttatc
     1381 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc
     1441 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta
     1501 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc
     1561 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat
     1621 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atatggctaa
     1681 caatggcttc ggaatgctta gttttgcata ttcagaaaaa gacaactata tagatagttt
     1741 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg
     1801 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg
     1861 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggatgcag
     1921 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg
     1981 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa
     2041 ttgaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg
     2101 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2161 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2221 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     2281 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2341 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2401 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2461 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2521 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2581 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2641 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2701 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2761 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2821 cgcagaaaaa aaggatctca agaagatcct ttgatctt
//
