LOCUS       sequence-231339-       10769 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-TP73-201
FEATURES             Location/Qualifiers
     source          1..10769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..548
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(84..107)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     481..500
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      589..598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             595..2247
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5834..5955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5845..5864)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5899..5918
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6200..6557
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6200..6391
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6223..6243)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6408..6543
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      6602..6611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6608..7540
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7592..8383
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7643..7662)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8253..8272
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8447..8495
                     /note="synthetic polyadenylation signal"
     promoter        8651..8755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8756..8824,8825..9616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8974..8993)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9774..10362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9854..9873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10478..10761
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatctaccgg gtaggggagg
       61 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
      121 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
      181 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
      241 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
      301 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
      361 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
      421 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
      481 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
      541 gcctttcgac ctgcagccca agcttggcaa tccggtactg ttggtaaagc caccatggaa
      601 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
      661 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
      721 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
      781 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
      841 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
      901 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
      961 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
     1021 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
     1081 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
     1141 cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc
     1201 ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc
     1261 cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca
     1321 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
     1381 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
     1441 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
     1501 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
     1561 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
     1621 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
     1681 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg
     1741 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
     1801 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
     1861 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
     1921 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
     1981 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
     2041 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
     2101 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
     2161 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
     2221 aagaagggcg gcaagatcgc cgtgtaattc tagttgttta aacgagagat ccccgagcag
     2281 taccgcatga ccatctggcg gggcctgcag gacctgaagc agggccacga ctacagcacc
     2341 gcgcagcagc tgctccgctc tagcaacgcg gccaccatct ccatcggcgg ctcaggggaa
     2401 ctgcagcgcc agcgggtcat ggaggccgtg cacttccgcg tgcgccacac catcaccatc
     2461 cccaaccgcg gcggcccagg cggcggccct gacgagtggg cggacttcgg cttcgacctg
     2521 cccgactgca aggcccgcaa gcagcccatc aaggaggagt tcacggaggc cgagatccac
     2581 tgagggcctc gcctggctgc agcctgcgcc accgcccaga gacccaagct gcctcccctc
     2641 tccttcctgt gtgtccaaaa ctgcctcagg aggcaggacc ttcgggctgt gcccggggaa
     2701 aggcaaggtc cggcccatcc ccaggcacct cacaggcccc aggaaaggcc cagccaccga
     2761 agccgcctgt ggacagcctg agtcacctgc agaaccttct ggagctgccc tagtgctggg
     2821 cttgtggggc gggggctggc ccactctcag ccctgccact gccccggcgt gctccatggc
     2881 aggcgtgggt ggggaccgca gcgtcggctc cgacttccag gcttcatcct agagactgtc
     2941 atctcccaac caggcgaggt ccttccaaag gaaaggatcc tctttgctga tggactgcca
     3001 aaaagtattt tgcgacatct tttggttctg gatagtagtg agcagccaag tgactgtgtc
     3061 tgaaacacca gtgtattttc agggaatgtc cctaactgcg tcttgcccgc gccgggggct
     3121 ggggactctc tctgctggac ttgggactgg cctctgcccc cagcacgctg tattctgcag
     3181 gaccgcctcc ttcctgcccc taacaacaac cacagtgttg ctgaaattgg agaaaactgg
     3241 ggagggcgca acccccccca ggcgcgggga agcatgtggt accgcctcag ccagtgcccc
     3301 tcagcctggc cacagtcgcc tctcctcggg gacccctcag cagaaaggga cagcctgtcc
     3361 ttagaggact ggaaattgtc aatatttgat aaaatgatac ccttttctac atggtgggtc
     3421 agcttttttt tttttttttt taactttctt tctcagcatt ctctttggag ttcaacctag
     3481 cgcccatgag ccaggctgag gaagctgagt gagaagccag gtgggcggga cttgttccca
     3541 ggaaggccgg gtggggagga agcctagagg gaaccccagg aagggcaaat ccaggcaaat
     3601 ctgcaggaat gctctgccat gggagcagct cctcccttgc cacggccacc ttctctagca
     3661 ctgcaaggtc cacagggcat tgctttcctt tctaggcggt ggcagtcagg gaacagactg
     3721 aggtaggtgt aggggggtct aggccttcgt ggagcacccc agggagttag taggccccgg
     3781 ggagacagag tctgcacagg ccctttctgg ggccacctcc atccacgagg agcagcctga
     3841 gccttggtgg ccgaaccttg accgtcccgg agcacagctt cagggcaggg aaccggagcc
     3901 cctggggggc ctcacgggtg tgacgaggcc cttcattgca ggcaggtggg ccaatgggag
     3961 ccctcaccca cgcaagccga gacaccaccc agagtgcagg ctgcctggcc ccttctggca
     4021 cggccagctc cacaccctct gcctagggta tgtgtggtcc taaggtctag gagcttcccc
     4081 tactaacatc tcccagaaaa agcagttaag cccctcaggg cacagcaagg ttagacacag
     4141 cccccatccc cagatcagga ctccatcttg ctaagtggca tcaccgtcac cagcctcccc
     4201 ttatttaaaa gcagcgactg gtgttgccgc aggtacctgg tctacgaaga cgcaggcgtc
     4261 cctctcccac cgtccacctc cccgggggcc gctgacagca cagtcgcctg ggtgcacgct
     4321 tgtgggggca gcaggaacgg ggctgtcggc tctcagggga tctggctgca gccagggcga
     4381 gggcctggcc cttccttcca gctccttccg gctccttcca gctgaagggc aggaagctct
     4441 ggccgcttag cttctagggt tccatctccc tagaaaggtg cccacgccca gggcatcagt
     4501 cagtagcggc agcagcagca gactcggggc tttcccaggg tggcgcagcc accccagctg
     4561 catgtcacct cagctctcca tcttattgcc attttgtaga tgaggaagct gagaccagaa
     4621 aggctaagac ccatgcccca ggcaccacac ccatctcttg ggggctgggc acctgctacc
     4681 cgaggccacc tcctgaagcc cccactcttc ccccatgttc cacttcagga gccgcggggg
     4741 cccatcctga cacccggggt tcctcagccc agcgcagatg tgcttcagtt ccagagggct
     4801 tgttgatttg tttcttaggt acgttacctg tccaccctga gtccagtgag gctgtcccaa
     4861 gagcccctgt agtgtgctcc tgggaagggc tgggggggct gggggggctg ggagaggccc
     4921 aggggcagct gtcactggaa ccccagccag atgtccaagg aagccggcca gaacacggag
     4981 cagccagatg gccccagctg cacctgtcta gggagcccat gcagcctcct tgcactggag
     5041 aagcagctgt gaaagtagac agagttgaga cttcgccgtg gtcaggagaa aatgcaaatt
     5101 cccaggaaca agaatccttt aagtgatatg tttttataaa actaaacaaa tcaacaaata
     5161 aatcttgaag gcggatggtt ttcccagcag tgcaggggtt ggagggaggc tgctggcact
     5221 cctggggcca agggggacag gcagtggtcc tgagtctgct cagagaggca aggcagaagg
     5281 agctcgccag gcaggtcagc tcacatctgt ccaagtcgct ctggtcagaa acagcgactc
     5341 tcccccattc ccccagcgtt cccaccaggc ctgggctgct gggaagccct tgctgtaccc
     5401 aggagcccga cccgcagtat cctggcacag agccacttgt cactcagaac agtcagtgtc
     5461 tccaacgcac aaacatccac tcctctgtta ccagttaaag cactttaatg ctttaaggtg
     5521 aaaacgaaat cccatccgtg tttttcgtgt aagatcgtgc ttctccgagc agtattaatg
     5581 gacgccctcc aatgacataa caactgtttt tggtaatgta atcttgggaa aatgtgttat
     5641 ttttttagct gtgtttcagt ggggattttt gtttttgtaa cataataaag tgtatgttcc
     5701 aatgaagtcg acctgcaggc atgcaagctg atccggctgc taacaaagcc cgaaaggaag
     5761 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcggccgctt
     5821 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5881 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5941 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6001 atgtgggagg tttttttaag caagtaaaac ctctacaaat gtggtaaaat cgaattttaa
     6061 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     6121 atttcacacc gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag
     6181 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     6241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6301 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6361 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6421 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6481 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6541 gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac caaaggctgg
     6601 agccaccatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc
     6661 tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga
     6721 ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta
     6781 cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct
     6841 gatcggaatg ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta
     6901 caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg
     6961 ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa
     7021 ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga
     7081 catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa
     7141 taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga
     7201 gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc
     7261 ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg
     7321 caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga
     7381 ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt
     7441 cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggtaagta
     7501 catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag accggtggtg ggagcggagg
     7561 tggcggatca ggtggcggag gctccggagg gattgaacaa gatggattgc acgcaggttc
     7621 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7681 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7741 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     7801 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7861 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7921 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7981 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     8041 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     8101 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     8161 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     8221 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     8281 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     8341 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     8401 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     8461 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt
     8521 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     8581 ccgtttctgc ggactggctt tctacgtaat ggtttcttag acgtcaggtg gcacttttcg
     8641 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8701 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8761 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8821 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8881 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8941 acgttttcca atgatgagca ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat
     9001 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9061 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9121 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa ctatcggagg
     9181 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9241 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9301 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9361 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9421 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9481 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9541 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9601 gattaagcat tggtaattcg aaatgaccga ccaagcgacg cccaaccggt atcagctcac
     9661 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9961 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10201 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10321 gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt
    10381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10441 ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct
    10501 ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg
    10561 ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag
    10621 gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
    10681 tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact
    10741 gtctgccctc gtagccatcg agataaacc
//
