LOCUS       sequence-231679-        9519 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-PTPN18-201
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     279..298
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             393..2045
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4382..4503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4393..4412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4447..4466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4748..5105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4748..4939
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4771..4791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4956..5091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5150..5159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5156..6088
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6140..6931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6191..6210)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6801..6820
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6995..7043
                     /note="synthetic polyadenylation signal"
     promoter        7199..7303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7304..7372,7373..8164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7522..7541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8322..8910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8402..8421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9026..9309
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(9366..9519,1..346)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9401..9424)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
       61 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
      121 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
      181 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
      241 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
      301 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
      361 cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg
      421 gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca
      481 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
      541 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
      601 atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca
      661 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
      721 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
      781 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
      841 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
      901 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
      961 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
     1021 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
     1081 agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt
     1141 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg
     1201 aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca
     1261 cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc
     1321 acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac
     1381 gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc
     1441 tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg
     1501 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
     1561 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     1621 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
     1681 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     1741 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     1801 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     1861 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     1921 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     1981 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     2041 tgtaattcta gttgtttaaa cgagctcggt ctaacgccag ttcctgcctg ttgcctcttg
     2101 tgagctcgga ctgctgatgc cccggtgctg ctgagcgccg tgcgcagaat ggaaacagtg
     2161 ggcctggatc aaagttaaag tttctcaggg tgggaaatgt gggggctttg ccccaatgac
     2221 tgtagcattc aaggcttgag gctggaggag gtagctaggg tatagtggct ggtgaggctg
     2281 cacagagcag attcaagaaa gaagatcagg aaggggcatg acccctgagt tatgaagggg
     2341 agaagggaca gatgagcttc cggagactgc tctcctcacc acacagcact agtccatcct
     2401 cagcacctga gcctccctca cttggacact caggggacca cacagagaag tggatggaca
     2461 cttcgccatc caggcagaac taagccaggc ataaccacag ccaagcagat taaccccagg
     2521 cagaccgata aaaagacctc cagataggca gacagacaga tggaccacca acctggacag
     2581 acagccaaag cttcagagat acagtccaca ggtggacaaa gggatcccca gccagagaga
     2641 gagagaccag ccaacagctt gatagaccag tgcagccaga gagaccacca aacagagccc
     2701 ccaaaagaca gacatctctg ctagctggac agccaggtgg accccctaag ttagattact
     2761 agacagatat aaacagatcc cctgctgaac agatacacag agttctcaga ccccaccccc
     2821 accctcaggt gggctggctg gctgacagac cttctggcca gacagactcc taaccaacca
     2881 gatggactgc cagacaggca gacatcagcc acatggaatc ctgacatccc agccagccgg
     2941 ccagactctc atcttgatgt cttgatggat ggaccccagc tagtcagaca tgatcctcca
     3001 gattgacaga caagtccccc aaatgagtac acatctccag ctattcagac agatggaccc
     3061 ccagcaaatc aggacctatc taggcagacc ccagccagac ccccgccaga cagactccca
     3121 accagactga ccccttacta ttcacacagc ctgccgagta gctgggacta caggtctaat
     3181 tttttttttt tttaagaaat gagtttttgc catgttgccc agactggtct tgaactccca
     3241 acctcaagca atcctcctgc ctcagcctcc caaagtgctg agattacagg tgtgagccac
     3301 caggctcagc cccctaagat ttgaaacact ttaaatggcc catggtaggg ttcctgctag
     3361 gataaaacat taagcggctg ttaaaagaaa taaaaggagg acacgtctct gtgcactggt
     3421 gtggacaaat ctccaagtca ctgcaaaatg gaaaaagtat aagatgctct ttccctgaac
     3481 ctcaagggtc ccgcccctct cactttcagg tctctggacc tctgactgac actgtgcctg
     3541 cccaggtccc tgtatgcact gccacagtgc cctgggcccc atgtccaccc ctgtcctgcc
     3601 cttctctggg atagggctgg ccttcctctg cctctgcctg gctgcatcca tggtcgatct
     3661 caagtgcctt ggcatgaact ccactctcct gcagccttca atcaaggaat gatggggatg
     3721 tgtacatacc ccaccccacc ccttggcagg gtgatgctga ggtgtggatt tttaacagtt
     3781 cccagacttt cccaggaggc ttgggtttgg gtgcccacag tgggagctgg tgtgatatca
     3841 taccttcgcc ggccgccttt ccttcctgtt ctctgtgccc ctactcccac tctagagctg
     3901 ccccgtttct ctgttttcgt gaaagagctg accctgtgct gcctcccact ctcccaatgc
     3961 ccctgccact cctgtgagcc tgctgctggt gaggtcggtg ctgacctctg tgttgctgga
     4021 taatgagtca tctatctctg gaggagaaga aaggcaggtc ctccacagcc ctgataaaat
     4081 ctccaagtct cccagtttcg ggtccctctc ctgggatgca gacccactgc ctgcccagct
     4141 ggtacgatcc acatgccctc ttcttgggaa taggggcatg ggaaagtgac taaagatact
     4201 gttctggctg ctgtgttcac tgtgagtaat aaactgtcca tttctccgat tcaagtcgac
     4261 ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     4321 ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg agcagacatg
     4381 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4441 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4501 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     4561 tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca aaatattaac
     4621 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4681 atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     4741 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5101 gcaaaaagct tgattcttct gacacaacag tctcgaacca aaggctggag ccaccatggc
     5161 ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc
     5221 tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca
     5281 cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca
     5341 cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg
     5401 taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac
     5461 cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg
     5521 ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca
     5581 tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga
     5641 tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt
     5701 cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta
     5761 cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga
     5821 gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc
     5881 ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt
     5941 ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa
     6001 gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt
     6061 cgtggagcgc gtgctgaaga acgagcagac cggtggtggg agcggaggtg gcggatcagg
     6121 tggcggaggc tccggaggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6181 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6241 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6301 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6361 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6421 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6481 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6541 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6601 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6661 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6721 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6781 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6841 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6901 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6961 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca
     7021 tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atcctctttg cgcttgcgtt
     7081 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     7141 actggctttc tacgtaatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7261 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7321 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7381 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7441 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7501 gatgagcact ttcaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7561 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7621 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7681 catgagtgat aacactgcgg ccaacttact tctgacaact atcggaggac cgaaggagct
     7741 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7801 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7861 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7921 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7981 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8041 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8101 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8161 gtaattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc aaaggcggta
     8221 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8761 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8881 cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc tacggggtct
     8941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9001 atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg atggcccttc
     9061 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     9121 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgattgcgg
     9181 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     9241 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     9301 agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga ggatcgagat
     9361 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     9421 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     9481 taggcgccaa ccggctccgt tctttggtgg ccccttcgc
//
