LOCUS       sequence-231702-       11491 bp    DNA     circular SYN 15-APR-2025
DEFINITION  N.benthamiana expression plasmid for PmCYP719A26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK
  TITLE     The biosynthetic origin of psychoactive kavalactones in kava.
  JOURNAL   Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0.
  PUBMED    31332312
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0."
COMMENT     Sequence Label: pJKW1270
FEATURES             Location/Qualifiers
     source          1..11491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    98..122
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     261..280
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     405..427
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     419..436
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     420..436
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        738..783
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      3086..3338
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3470..3815
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    4442..4618
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        5184..5363
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5216..5399
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5420..6214
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5474..5493)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6084..6103
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6600..6852
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6943..6967
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      7335..7966
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8056..9204)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9503..10297)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10887..10904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11138..11157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
       61 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
      121 caaacactga tagtttgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt
      181 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      241 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgccat tcaggctgcg
      301 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      361 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      421 taaaacgacg gccagtgaat tgttaattaa gaattcgagc tccaccgcgg aaacctcctc
      481 ggattccatt gcccagctat ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc
      541 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      601 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      661 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      721 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      781 aggtattaaa atcttaatag gttttgataa aagcgaacgt ggggaaaccc gaaccaaacc
      841 ttcttctaaa ctctctctca tctctcttaa agcaaacttc tctcttgtct ttcttgcgtg
      901 agcgatcttc aacgttgtca gatcgtgctt cggcaccagt acaacgtttt ctttcactga
      961 agcgaaatca aagatctctt tgtggacacg tagtgcggcg ccattaaata acgtgtactt
     1021 gtcctattct tgtcggtgtg gtcttgggaa aagaaagctt gctggaggct gctgttcagc
     1081 cccatacatt acttgttacg attctgctga ctttcggcgg gtgcaatatc tctacttctg
     1141 cttgacgagg tattgttgcc tgtacttctt tcttcttctt cttgctgatt ggttctataa
     1201 gaaatctagt attttctttg aaacagagtt ttcccgtggt tttcgaactt ggagaaagat
     1261 tgttaagctt ctgtatattc tgcccaaatt cgcgaccggt atggagcaag ctcaatgggt
     1321 cgacccaact ctgctccctg catttgtggg catcatcttc ttcttccttg gcatgttctt
     1381 tggaaggagt tctttgggag ctgggaaggg tgcagcgcct agaagcacca gttctaccga
     1441 gtggccagac ggccctccaa agctgcccat catcggcaac ctgcaccagc tcaacaaagg
     1501 cggggagctg gtccaccaca acctcgccaa gctcgcccag tcctacgacc gcgccatgac
     1561 catctgggtc ggcagctggg gccccatgat cgtcgtcagc gacgccgacc ttgcatggga
     1621 ggtcctcgtc accaagtcgc cggacttcgc cggccgggtg ctctccaagc tctcgcactt
     1681 gttcaacgcc aactacaaca ccgtcgtcgc ctacgacgcc gggccgcaat ggcagtcgct
     1741 ccggcgaggt ctgcagcacg ggccgctcgg ccccgcccat gtttctgcgc aggctcgttt
     1801 ccacgaagaa gacatgaagc tcctggtgag cgacatgatg agagcagcac agaaaggtgg
     1861 tagcaatgga gtggttgaac ctctggccta tgtccggcga gccactatcc gatttctgtc
     1921 tcgtctatgc tttggggagg ccttcaacga cgaggcgttc gtggagggga tggacgaagc
     1981 agtggaggag accatcggag ccactggcca tgcacgcatc ctcgacgcct tctacttcac
     2041 tcgccacctc cctatcatcc gccgcagctt catagatacc gtcaacgcca agaagaagat
     2101 cgagagcctt gtccggccgt tgctctcccg gccggcgcca ccggggtctt acctccactt
     2161 cctcctttcc accgacgcgc cggagaatat gatcatcttt cgaatattcg aagtctactt
     2221 gctgggcgtg gacagcaccg cctccaccac cacatgggct ctcgccttcc tcgtctccaa
     2281 ccaacaggcg caggagaagc tccacaatga gctcgcccag tactgcgcca gccagaacaa
     2341 tcagatcatc aaagcagacg acgtcggaaa gctgtcgtac ctgctcgggg tagtgaagga
     2401 gacgatgagg atgaagccga tagcgccgct ggccgtcccc cacaagacgc tcaaggagac
     2461 gatgctcgac ggaaagaggg tggcggcggg aacgacggtg gtagtgaacc tctatgccgt
     2521 ccactacaac ccgaagctat ggccggagcc ggagcagttc cgcccggaga ggttcgtggt
     2581 cggcgccagc ggcggcaatg gtggggggtc ttccgagtac atgctgcagt cgtacctgcc
     2641 ctttggaggg gggatgaggt cctgcgcagg gatggaggtg ggaaagttgc aggtggcgat
     2701 ggtcgtggcc aaccttgtga tggcatttaa atggttgccg gaggaggagg ggaagatgcc
     2761 ggacctggct gaagacatga ccttcgtgct catgatgaag aagccattgg ctgccaaaat
     2821 cgttccacgt gcatgactcg aggcctttaa ctctggtttc attaaatttt ctttagtttg
     2881 aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg tgctcagagt
     2941 gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt cgtcccttca
     3001 gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaaa gaccgggaat
     3061 tcgatatcaa gcttatcgac ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
     3121 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3181 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3241 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3301 taaattatcg cgcgcggtgt catctatgtt actagatctc tagagtctca agcttggcgc
     3361 gccagcttgg cgtaatcatg gtcatagctg ttgcgattaa gaattcgagc tcggtacccc
     3421 cctactccaa aaatgtcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     3481 aacaaagggt aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     3541 tcgaaaggac agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
     3601 aggctatcat tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     3661 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     3721 acatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     3781 ctatataagg aagttcattt catttggaga ggacagccca agcttcgact ctagaggatc
     3841 cccttaaatc gatatggaac gagctataca aggaaacgac gctagggaac aagctaacag
     3901 tgaacgttgg gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag
     3961 tccgagttgg actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc
     4021 ccttggtttc aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata
     4081 cgacaggacg gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa
     4141 ctatgcagca tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt
     4201 tcgaggagtt agtatcaccg tttctggagg gtctcgaact cttcagcatc tctgtgagat
     4261 ggcaattcgg tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt
     4321 atcaagagga tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagtcgaggg
     4381 cgagctcgaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
     4441 ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg
     4501 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     4561 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc
     4621 ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg
     4681 agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga
     4741 tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt
     4801 ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga
     4861 agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag ggaatgcttg
     4921 tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc
     4981 ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat
     5041 aaaacctcac caaaatacga aagagttctt aactctaaag ataaaagatg gcgcgtggcc
     5101 ggccatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag
     5161 ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca gccgcgggtt
     5221 tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg
     5281 cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat
     5341 aaattcccct cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcac
     5401 cggatctgga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5461 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5521 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5581 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5641 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5701 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5761 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5821 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5881 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5941 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     6001 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6061 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6121 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6181 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6241 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6301 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6361 ctcatgctgg agttcttcgc ccacgggatc tctgcggaac aggcggtcga aggtgccgat
     6421 atcattacga cagcaacggc cgacaagcac aacgccacga tcctgagcga caatatgatc
     6481 gcggcgtcca catcaacggc gtcggcggcg actgcccagg caagaccgag atgcaccgcg
     6541 atatcttgct gcgttcggat attttcgtgg agttcccgcc acagacccgg atgatccccg
     6601 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     6661 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     6721 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     6781 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     6841 tgttactaga tcgggggccg gccctcactg gtgaaaagaa aaaccacccc agtacattaa
     6901 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     6961 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     7021 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     7081 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     7141 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     7201 gatcaaatat catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc
     7261 ttaaccgtga cagagtagac aggctgtctc gcggccgagg ggcgcagccc ctggggggat
     7321 gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
     7381 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
     7441 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
     7501 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
     7561 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
     7621 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
     7681 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
     7741 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
     7801 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
     7861 gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata
     7921 gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgctcgg
     7981 tcttgccttg ctcgtcggtg atgtacacta gtcgctggct gctgaacccc cagccggaac
     8041 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     8101 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     8161 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     8221 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     8281 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     8341 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     8401 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     8461 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     8521 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     8581 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     8641 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     8701 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     8761 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     8821 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     8881 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     8941 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
     9001 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     9061 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     9121 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     9181 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     9241 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     9301 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     9361 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
     9421 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
     9481 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
     9541 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
     9601 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
     9661 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
     9721 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
     9781 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
     9841 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
     9901 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
     9961 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    10021 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    10081 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    10141 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    10201 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    10261 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    10321 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    10381 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    10441 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
    10501 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
    10561 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
    10621 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
    10681 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
    10741 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
    10801 ctcaggccgc agtcggtaac tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10861 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10921 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10981 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    11041 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    11101 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11161 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11221 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    11281 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11341 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11401 ctggcagcag gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
    11461 ggacgggccc ccggcgccag atctggggaa c
//
