LOCUS       sequence-231715-        2555 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of PYL-SfGFP-Emerin, lentivirus vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, 
            La Russa M, Qi LS
  TITLE     CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear 
            Organization.
  JOURNAL   Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 
            10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
  PUBMED    30318144
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.013"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1405-1417.e14"
COMMENT     Sequence Label: pHR-SFFV-PYL1-sfGFP-Emerin
FEATURES             Location/Qualifiers
     source          1..2555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..492
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1062..1772
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1062..1772
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1089..1117)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1712..1732
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
       61 tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
      121 ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa
      181 acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc
      241 gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc
      301 agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct
      361 gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt
      421 cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact
      481 gagtcatggg tgggggcgcg ccaactcaag acgaattcac ccaactctcc caatcaatcg
      541 ccgagttcca cacgtaccaa ctcggtaacg gccgttgctc atctctccta gctcagcgaa
      601 tccacgcgcc gccggaaaca gtatggtccg tggtgagacg tttcgatagg ccacagattt
      661 acaaacactt catcaaaagc tgtaacgtga gtgaagattt cgagatgcga gtgggatgca
      721 cgcgcgacgt gaacgtgata agtggattac cggcgaatac gtctcgagag agattagatc
      781 tgttggacga tgatcggaga gtgactgggt ttagtataac cggtggtgaa cataggctga
      841 ggaattataa atcggttacg acggttcata gatttgagaa agaagaagaa gaagaaagga
      901 tctggaccgt tgttttggaa tcttatgttg ttgatgtacc ggaaggtaat tcggaggaag
      961 atacgagatt gtttgctgat acggttatta gattgaatct tcagaaactt gcttcgatca
     1021 ctgaagctat gaacgtggat ccaggtggag gtggaagcgg tagcaaagga gaagaacttt
     1081 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1141 ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt aaatttattt
     1201 gcactactgg aaaactacct gttccgtggc caacacttgt cactactctg acctatggtg
     1261 ttcaatgctt ttcccgttat ccggatcaca tgaaacggca tgactttttc aagagtgcca
     1321 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg acctacaaga
     1381 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaagggta
     1441 ttgattttaa agaagatgga aacattcttg gacacaaact cgagtacaac tttaactcac
     1501 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
     1561 gccacaacgt tgaagatggt tccgttcaac tagcagacca ttatcaacaa aatactccaa
     1621 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgtccttt
     1681 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
     1741 ggattacaca tggcatggat gagctctaca aaggtggagg tcggaccgaa atggacaact
     1801 acgcagatct ttcggatacc gagctgacca ccttgctgcg ccggtacaac atcccgcacg
     1861 ggcctgtagt aggatcaact cgtaggcttt acgagaagaa gatcttcgag tacgagaccc
     1921 agaggcggcg gctctcgccc cccagctcgt ccgccgcctc ctcttatagc ttctctgact
     1981 tgaattcgac tagaggggat gcagatatgt atgatcttcc caagaaagag gacgctttac
     2041 tctaccagag caagggctac aatgacgact actatgaaga gagctacttc accaccagga
     2101 cttatgggga gcccgagtct gccggcccgt ccagggctgt ccgccagtca gtgacttcat
     2161 tcccagatgc tgacgctttc catcaccagg tgcatgatga cgatcttttg tcttcttctg
     2221 aagaggagtg caaggatagg gaacgcccca tgtacggccg ggacagtgcc taccagagca
     2281 tcacgcacta ccgccctgtt tcagcctcca ggagctccct ggacctgtcc tattatccta
     2341 cttcctcctc cacctctttt atgtcctcct catcatcttc ctcttcatgg ctcacccgcc
     2401 gtgccatccg gcctgaaaac cgtgctcctg gggctgggct gggccaggat cgccaggtcc
     2461 cgctctgggg ccagctgctg cttttcctgg tctttgtgat cgtcctcttc ttcatttacc
     2521 acttcatgca ggctgaagaa ggcaacccct tctag
//
