LOCUS       sequence-231754-        5629 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM
  TITLE     Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha
            family of nuclear import receptors.
  JOURNAL   BMC Cell Biol. 2010  . 11():63.
  PUBMED    20701745
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol. 2010  . 11():63."
COMMENT     Sequence Label: pCMVTNT-T7-KPNA3
FEATURES             Location/Qualifiers
     source          1..5629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        523..726
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     677..697
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     723..747
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..994
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     977..996
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1039..1057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1112..1121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2748..2869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2759..2778)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2813..2832
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3050..3505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3137..3156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3347..3368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3572..3591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3691..3713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3751..3769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3837..3941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3942..4010,4011..4802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4160..4179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4973..5561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5462..5481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
       61 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
      121 tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata gtaatcaatt
      181 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      241 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      301 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      361 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      421 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      481 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      541 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      601 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      661 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      721 agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt
      781 taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagaagt
      841 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
      901 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt
      961 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc
     1021 tcttaaggct agagtactta atacgactca ctataggcta gcatttaggt gacactatag
     1081 aatacaagct acttgttctt tttgcactcg agccaccatg gccagcatga ccggcggcca
     1141 gcagatgggc gaattcgccg agaaccccag cttggagaac caccgcatca agagcttcaa
     1201 gaacaagggc cgcgatgtgg aaacaatgcg aagacataga aatgaagtga cagtggaact
     1261 gcggaagaac aaaagagatg aacacttatt gaaaaagaga aatgttcccc aagaagaaag
     1321 tctagaagat tcagatgttg atgctgattt taaagcacaa aatgtaaccc tagaagctat
     1381 attgcagaat gccacaagtg ataacccagt ggtccaattg agtgctgtcc aggcagcaag
     1441 aaaactgtta tccagtgaca gaaatccacc gattgatgac ttaataaaat ctgggatttt
     1501 accaattcta gtcaaatgtc tagaaaggga tgataatcct tcattacagt ttgaagctgc
     1561 ttgggcatta actaacatag catcaggaac ttctgcacag actcaagctg ttgtgcagtc
     1621 taatgcagta cctctttttc tgagacttct tcgttcacca catcagaatg tttgtgaaca
     1681 agcagtatgg gctttgggaa acattatagg tgatggtcct caatgtagag attatgtcat
     1741 atcactggga gttgtcaaac ctcttctgtc cttcatcagt ccctccatcc ccatcacctt
     1801 ccttcggaac gtcacatggg tcattgtcaa tctctgcagg aataaggatc ccccaccgcc
     1861 tatggagaca gttcaggaga ttttgccagc tttatgtgtc ctcatatacc atacagatat
     1921 aaacattctt gtagacactg tttgggctct gtcatacttg acagatggag gtaatgaaca
     1981 gatacagatg gttattgatt caggagttgt gccctttctt gtgccccttc tgagccatca
     2041 ggaagtcaaa gttcaaacag cagccctcag agcagttggc aacatagtga ctggcaccga
     2101 cgagcagacc caggttgttc tcaattgtga tgtcctgtca cacttcccaa atctcttatc
     2161 acacccaaaa gagaagataa ataaggaagc agtgtggttc ctttccaaca taacagcagg
     2221 caaccagcaa caagttcaag ctgtaataga tgctggatta attcctatga taattcatca
     2281 gcttgctaag ggggactttg gaacacaaaa agaagctgct tgggcaatca gcaacttaac
     2341 aataagtggc agaaaagatc aggttgagta ccttgtacag cagaatgtaa taccaccgtt
     2401 ctgtaattta ctgtcagtga aagattctca agtggttcag gtggttctag atggtctaaa
     2461 aaacattctg ataatggccg gtgatgaagc aagcacaata gctgaaataa tagaggaatg
     2521 tggaggtttg gagaaaattg aagttttaca gcaacatgaa aatgaagaca tatataaatt
     2581 agcatttgaa atcatagatc agtatttctc tggtgatgat attgatgaag atccctgcct
     2641 cattcctgaa gcaacacaag gaggtaccta caattttgat ccaacagcca accttcaaac
     2701 aaaagaattt aatttttaac ccgggcggcc gcttcgagca gacatgataa gatacattga
     2761 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2821 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2881 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     2941 aaacctctac aaatgtggta aaatcgataa ggatccgggc tggcgtaata gcgaagaggc
     3001 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     3061 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3121 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3181 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3241 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3301 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3361 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3421 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3481 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     3541 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3601 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3661 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3721 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3781 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3841 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4021 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4081 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4141 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4201 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4261 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4321 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4381 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4441 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4501 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4561 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4621 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4681 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4741 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4801 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4861 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4921 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5101 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5581 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgaca
//
