LOCUS       sequence-231759-        9896 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-RASSF4-201
FEATURES             Location/Qualifiers
     source          1..9896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..1683
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4397..4518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4408..4427)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4462..4481
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4763..5120
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4763..4954
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4786..4806)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4971..5106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5165..5174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5171..6103
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6155..6946
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6206..6225)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6816..6835
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7010..7058
                     /note="synthetic polyadenylation signal"
     promoter        7214..7318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7319..7387,7388..8179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7537..7556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8337..8925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8417..8436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9041..9324
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        9381..9880
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9416..9439)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9813..9832
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc
       61 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
      121 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
      181 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
      241 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
      301 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
      361 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
      421 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
      481 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
      541 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
      601 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
      661 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
      721 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
      781 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
      841 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
      901 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
      961 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     1021 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     1081 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     1141 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     1201 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     1261 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     1321 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     1381 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     1441 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     1501 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     1561 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     1621 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     1681 taattctagt tgtttaaacg agctcgctgg ccaacacctg cctcttccaa agtccccagc
     1741 agtggcaggt gtacactgag ccctggttgc tggccccggc cggtcacatt gactgatggc
     1801 caccgcctga cgaatcgagt gcctgtgtgt ctacctctct gaagcctgag caccatgatt
     1861 cccacagcca gctcttggct ccaagatgag cacccacagg aagccgaccc aggcctgagg
     1921 ggccaggaac ttgctgggtc agatctgtgt ggccagccct gtccacacca tgcctctcct
     1981 gcactggaga gcagtgctgg cccagcccct gcggcttagg cttcatctgc ttgcacattg
     2041 cctgtcccag agcccctgtg ggtccacaag cccctgtcct cttccttcat atgagattct
     2101 tgtctgccct catatcacgc tgccccacag gaatgctgct gggaaaagca gggcctgcca
     2161 gcaggtatga gatctagcct gctttcagcc atcaccttgc cacagtgtcc ccggcttcta
     2221 agcctccaat atcaccctgt gagcctcgca cagctcagcc ccaacacaga ggtgagacca
     2281 ggaataaggc cacaagtatc tcactttctc tgcagaaatc aatctttact tcatcagaga
     2341 gacctaaagc gattcttaca aggagcttgc tgcaagaaac acggtcattc aatcacattg
     2401 aggagggtcc acatggcatt gagagggtgc tgcccgctca atgcccagca gcagctctgg
     2461 aaggcagtgc tcagccccat caccactgtc ccgtggatgc ctgtgtacct cttgcctttt
     2521 ctgggcttgc gtttctctcc tctagtgggt ggggatgact ttcaatgact ttcaatactt
     2581 cccctgaagg aagaatgata aggagaaatg tctgttttga ggaaagggct ttgaattccc
     2641 cagatactga acaatttgtg tttgtgactg atggagaatt tcaggaatga atgagaaagc
     2701 ctttgcgaaa ctatgcaaca gtttacatca gtcatgtgaa gtatttgtct aaaacagagc
     2761 aaactgaaga ccaaattatt ctcctgttga ggtccgtgga tggcagattt aaagggaaga
     2821 accacaaagg cttgcaaaga taggagaggc tccatctcta atgcatgtag aagctcctta
     2881 cgggtgccca tcaagagcat agcttggaag ccaccatgct gtgcggaact gcgtcagggc
     2941 aaatgtcaca gcaggatttc cccaacccag ctccatcatc acagacacag agagctgcag
     3001 gggaggcctg cccactgttt tgtcgactct gccctcctct ggcagcatag atccttaggt
     3061 gctcaataaa ggtgtgctgt attgaactga agaagtgaga accaagcttt tttttttttt
     3121 tttgctttct gtgcatccac cctgaccccc ggctctaact tctgaaactc ttatcctaaa
     3181 ggatgtgtgg ccatgtttgg cagaagagcc acaactcgct gctctaggag ccgacatgac
     3241 tccttcattt ctgtgcatgc cctggggact agggtggagc ctgaggagag gctgccatgg
     3301 aacacgcagg cgtcactggt gggagcctag aggtgacctg gatgatgagc aatagagtca
     3361 ccccgagtca cagaatctta gcatcaggag ggccccaaga atcttccagg ccaggggctc
     3421 aggtgggatg gaagcagggc ctaggagagg gtacaaaaca aatcaaacag gagctcccag
     3481 gtaattgtac tcccaagcta ttatttacaa tctcaggtgg aggacagctc ctgtcccatt
     3541 gttcagctgt aactgagtga ctgccttgtg ccaggcctgt gctcagccct ggaggcacaa
     3601 agatggagcc agtctgtcct gtgtctaggg gtcttctcat caggagaaga aggccagaca
     3661 ctggaagggt caagagatca gctgagcaac gaaccggcaa agctcctggg agtgaggtgg
     3721 aggccaaggc agagttggct aagaagtgca tgtggcgtca caggccaaga tgcacctgcc
     3781 agctaggagc aggccaggtc ctggcacaca ggaagccagt ggagcaggag accctcccta
     3841 agtaccagcc tgtttggttg gttggtttgc ttttgaaagt tattacaata ttatttaagg
     3901 ccttttcgga taagttcact tttaaagtag gtgttatcaa tctaatttct gaacgttatg
     3961 ggtcagaaaa atcactagca agtggctcag aaaaagggaa aaggtacact tctgcaatca
     4021 gctgaggagt tcacaccttt ttttctaaat caacatcata tttgctgttc agaggcaatc
     4081 tgaaaagctg caaattcagc tcacctcaac cccataattt caaacgttag aggctggtct
     4141 ctgtggtttc ctactggttc ctgtggggag ctcccgcttt ctttctccat agagcttagg
     4201 gtatttctca tatgccccat tggctaccta gtgtctcagg taataaacgt atccgaagat
     4261 aaagcaaaag tcgacctgca ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg
     4321 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcggccg
     4381 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4441 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4501 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4561 gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa aatcgaattt
     4621 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     4681 ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa
     4741 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4801 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4861 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4921 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4981 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5041 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5101 gaggcctagg cttttgcaaa aagcttgatt cttctgacac aacagtctcg aaccaaaggc
     5161 tggagccacc atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg
     5221 gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta
     5281 tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag
     5341 ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga
     5401 tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca
     5461 ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt
     5521 gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat
     5581 caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc
     5641 tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga
     5701 gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga
     5761 ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct
     5821 ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt
     5881 ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc
     5941 cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga
     6001 gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa
     6061 gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagaccggtg gtgggagcgg
     6121 aggtggcgga tcaggtggcg gaggctccgg agggattgaa caagatggat tgcacgcagg
     6181 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6241 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6301 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6361 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6421 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6481 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6541 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6601 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6661 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6721 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6781 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6841 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6901 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6961 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc
     7021 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatcct
     7081 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     7141 gcaccgtttc tgcggactgg ctttctacgt aatggtttct tagacgtcag gtggcacttt
     7201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7501 agaacgtttt ccaatgatga gcactttcaa agttctgcta tgtggcgcgg tattatcccg
     7561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caactatcgg
     7741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8161 actgattaag cattggtaat tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct
     8221 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8281 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8521 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8761 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc
     8941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9001 agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac aggaacgcac
     9061 gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa
     9121 gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat
     9181 caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg
     9241 tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt
     9301 actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc
     9361 gtagaggatc gagatctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     9421 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     9481 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     9541 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     9601 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     9661 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     9721 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     9781 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     9841 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagct
//
