LOCUS       sequence-231783-        5329 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mouse GFR alpha 2 In situ hybridization probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD
  TITLE     Molecular identification of rapidly adapting mechanoreceptors and 
            their developmental dependence on ret signaling.
  JOURNAL   Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003.
  PUBMED    20064391
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.11"; journalName: "Neuron"; date: 
            "2009-12-24- 24"; volume: "64"; issue: "6"; pages: "841-56"
COMMENT     Sequence Label: pCR2.1 GFR alpha 2
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1762..1781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1763..1781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1788..1805)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1788..1804)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1797..1819)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1945..2373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2032..2051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2242..2263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             2717..3511
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2771..2790)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3381..3400
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3529..3597,3598..4389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3747..3766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4560..5148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5049..5068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5302..5319
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttaacatg
      301 atcttggcaa acgccttctg cctcttcttc tttttagacg aaaccctccg ctctttggcc
      361 agcccttcct ctccgcaggg ctctgagctc cacggctggc gcccccaagt ggactgtgtc
      421 cgggccaatg agctgtgtgc ggctgaatcc aactgcagct ccaggtaccg cacccttcgg
      481 cagtgcctgg caggccggga tcgcaatacc atgctggcca ataaggagtg ccaggcggcc
      541 ctggaggtct tgcaggaaag cccattgtat gactgccgct gcaagcgggg catgaagaag
      601 gagccgcagt gtctgcagat ctattggagc atccatctgg ggctgacgga gggtgaggag
      661 ttctacgaag cttcgcccta tgagcctgtg acctcccgcc tctcggacat cttcaggctc
      721 gcttcaatct tctcagggac aggggcagac ccggtggtca gtgccaagag caaccactgc
      781 ctggatgccg ccaaggcctg caacctgaac gacaactgca agaagctccg ctcctcctac
      841 atctccatct gcaaccgcga gatctctccc accgaacgct gcaaccgccg caagtgccac
      901 aaggccctgc gccagttctt cgaccgtgtg cccagcgagt atacctaccg catgctcttc
      961 tgctcctgtc aggaccaggc atgcgccgag cgtcgccggc aaaccatcct gcccagctgt
     1021 tcctatgagg acaaagagaa gcccaattgc ttggacctgc gcagcctgtg tcgtacagac
     1081 cacttgtgcc ggtcccgcct ggcagacttc cacgccaact gtcgagcctc ctaccggaca
     1141 atcaccagct gccctgcgga caactaccag gcatgtctgg gctcctatgc tggcatgatt
     1201 gggtttgata tgacaccgaa ctatgtggac tccaacccca cgggcatcgt ggtgtctccc
     1261 tggtgcaatt gtcgtggcag tgggaacatg gaagaagagt gtgagaagtt cctcaaggac
     1321 ttcacagaaa acccatgcct ccggaatgcc attcaagcct ttggcaatgg cacagatgtg
     1381 aacatgtctc ccaaaggccc cacattttca gctacccagg cccctcgggt agagaaaact
     1441 ccttcactgc cagatgacct cagtgatagc accagcctgg ggaccagtgt catcaccacc
     1501 tgcacatcta tccaggagca agggctgaag gccaacaact ccaaagagtt aagcatgtgt
     1561 ttcacagagc tcacgacaaa tatcagccca gggagtaaaa aggtgatcaa actttactca
     1621 ggctcctgca gagccagact gtcgactgcc ttgactgccc tcccactcct gatggtgacc
     1681 ttggcccagt agaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat
     1741 gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     1801 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1861 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1921 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2281 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2341 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg
     2401 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     2461 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     2521 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     2581 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     2641 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     2701 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2761 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2821 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2881 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2941 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3001 cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat
     3061 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3121 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3181 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3241 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3301 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3361 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3421 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3481 cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa
     3541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4681 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
