LOCUS       sequence-232159-       11615 bp    DNA     circular SYN 14-APR-2025
DEFINITION  rfp ""test"" strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1071
FEATURES             Location/Qualifiers
     source          1..11615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(58..849,850..918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     681..700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(919..1023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1091..1109
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1147..1169)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1269..1288
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1702..1718
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1703..1719
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1747..1841
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      1944..2030
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2081..2128
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2196..2432)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(2758..2763,2764..2780,2781..2786))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    2877..2924
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3062..3739)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             3746..3757
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(4087..4093,4094..4110,4111..4116))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(4675..5553)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5580..5864
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5787..5806
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             5891..5913
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(5925..5951,5952..5954,5955..8441,8442..8444,
                     8445..10028)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5925..10028
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10038..10127
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  10283..10448
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            10607..10715
                     /note="incP origin of transfer"
     rep_origin      10984..11372
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
       61 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      181 tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
      241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1081 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgacgacgg
     1141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     1261 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1321 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     1381 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctag aggaccagcc
     1441 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcaggt
     1501 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     1561 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     1621 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     1681 tattaaagag gggcgtgggg ttcgaggtcg acggtatcga taagctagct taattagctg
     1741 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1801 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag actgcgatga
     1861 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggggt
     1921 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgctag
     2041 gagcttgcgg cccggacgat gagctcgaat tggggatctt gaagtaccta ttccgaagtt
     2101 cctattctct agaaagtata ggaacttcag agcgcttttg aagctgatgc tcgagtgctt
     2161 aaaaacttac tcaatggaat aattctagat aattcttagg ccacacgttc aagtgcagcc
     2221 acaggataaa tttgcactga gcctgggtgg gattcggact cgaccgcata gccttcagga
     2281 gtgagttttg tgcaatacca accgacgact tgaccctgcc aagcggcacc agatttcttg
     2341 cgtacgcgat cccctaagcc aaaggtggca ctcaggggaa gcgcaaactg ccctgcaacg
     2401 ggagcgttgg cttcatcgct actttgaccc atgtcgaatc cttcttgtga atctattatg
     2461 gcgacaagca aatcgagctc tgactgccta ccccacaaca actatcagaa agcaccagca
     2521 caacggctgc ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     2581 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     2641 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2701 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     2761 ttacgaaccg aacaggctta tgtcaactgg gttcgtgaat tatccattgc tgttgacaaa
     2821 gggaatcagg ctcgagagtt caacagacag ccttcgttct tatggccgtt caggaggaag
     2881 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcttgaga agagaaaaga
     2941 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
     3001 gttttttggc tgaaacgaat gacgaaaagg ctgtctgagc aaatccagga ggtcgttttt
     3061 attaagcacc ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt
     3121 cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa
     3181 ccggtttttt agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca
     3241 gtttcagacg cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg
     3301 tggaagcttc ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg
     3361 taccacgcag tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa
     3421 cggtaacaac accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga
     3481 aggacagttt caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt
     3541 actggaactg cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt
     3601 tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga
     3661 actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa
     3721 cgtcttcgct actcgccatg gtacctttct cctctttaat gaattcagat ctattatacc
     3781 taggactgag ctagctgtca aattcaccac cctgaattga ctctcaaacc gtattagccc
     3841 ggtattttgg aaatagcgga agcactgact tttgttatca ataaaaaagg ccccccgtta
     3901 gggaggcctt attgttcgtc tactcggaag agcgagagac aacagaacgg tcagccacat
     3961 gaattcaaaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
     4021 tatttaaact tgctatgctg tttccagcat agctcttaaa cagaccgcta aactgaaagt
     4081 tccacacatt atacgagccg gatgattaat tgtcaacagc tcatttcaga atatttgcca
     4141 gaaccggaat tctttcagct cagtcgatag gtagtaggca agagtagtcg cacctttggt
     4201 cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttccc caaaagtaaa
     4261 aacccgccga agcgggtttt tacgtaaaac aggtgaaact gaccagacga gaaggctttg
     4321 gaggtgataa tggggctcaa ggacccggga ggcagatagg taggcatggc ccccattttc
     4381 aatacaagca acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc
     4441 gctccggcaa ttaaaaaagc ggctaaccac gccgcttttt ttacgtctgc agagctcata
     4501 ggcaagcgaa tcgtgatgcc tcttagccag tactagttta tgacaacttg acggctacat
     4561 cattcacttt ttcttcacaa ccggcacgga actcgctggt cccacagaat gagcatcatg
     4621 gcactagttt atgacaactt gacggctaca cagaatgagc atcatggcac tagtttatga
     4681 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     4741 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     4801 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
     4861 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
     4921 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     4981 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     5041 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
     5101 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
     5161 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     5221 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
     5281 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
     5341 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     5401 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     5461 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     5521 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
     5581 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
     5641 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
     5701 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
     5761 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
     5821 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc
     5881 tagaaataat tttgtttaac tttaagaagg agatatacat acccatggac aagaagtaca
     5941 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     6001 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     6061 acctgatcgg cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga
     6121 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     6181 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     6241 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     6301 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     6361 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     6421 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     6481 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     6541 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     6601 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc
     6661 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     6721 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     6781 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     6841 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     6901 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     6961 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg
     7021 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     7081 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     7141 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     7201 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     7261 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     7321 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     7381 ccagcgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     7441 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca
     7501 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     7561 aagccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     7621 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     7681 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     7741 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     7801 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     7861 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     7921 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     7981 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     8041 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     8101 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     8161 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     8221 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     8281 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     8341 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     8401 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     8461 ttctgaagga cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca
     8521 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc
     8581 tgctgaatgc caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag
     8641 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     8701 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     8761 acgacaaact gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     8821 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     8881 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     8941 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     9001 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     9061 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     9121 acggcgaaac aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag
     9181 tgctgtctat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     9241 gcaaagagtc tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact
     9301 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     9361 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     9421 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     9481 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     9541 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     9601 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     9661 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc
     9721 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     9781 atctggacaa ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg
     9841 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     9901 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     9961 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
    10021 gaggcgacgc ggccgcggag cagaaactca tctctgaaga agatctggaa caaaagttga
    10081 tttcagaaga agatctggaa cagaagctca tctctgagga agatctgtaa taaggcgcgc
    10141 ctccttaatg ggacttgcag cctcggtacc aaattccaga aaagacaccc gaaagggtgt
    10201 tttttcgttt tggtcccaca gaatgagcat catggcacta gtcgacatcg gagaagagta
    10261 cggctctttt aaccgcctca agaaccagat aagtgaaatc tagttccaaa ctattttgtc
    10321 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
    10381 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
    10441 cgcccacagc ggggcatttt tcttcctgtt atgtttgggc gctgcattaa tgaatcggcc
    10501 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgacc
    10561 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcaga gcttatcggc cagcctcgca
    10621 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
    10681 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
    10741 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
    10801 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaagt ataccttaag
    10861 gaatcccctg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10921 cgtaaaaagg ccgcactccc tgcctctgtc atcacgatac tgtgatgcca tggctaattc
    10981 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
    11041 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa
    11101 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
    11161 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
    11221 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
    11281 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
    11341 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat
    11401 cttgcggccg caaaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc aataactgcc tttgatcttt
    11521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11581 ttatcaaaaa ggatcttcac ctagatcctt ttaaa
//
