LOCUS       sequence-232343-        3888 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial mClover promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mClover-Sh_ble
FEATURES             Location/Qualifiers
     source          1..3888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    1814..1832
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1898..1915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2069..2657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2149..2168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2828..3619,3620..3688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3451..3470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3689..3793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3861..3879
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct mgggkrtycy
      361 cgsrtayygn gctcgaattc taaggaggaa aaaaaatggt gagtaaaggc gaagaactgt
      421 ttaccggcgt agtaccaatt cttgttgaac ttgatggcga cgtgaatggt cacaaattct
      481 cggtccgcgg tgaaggcgag ggtgacgcaa ccaacggcaa gctgacgctg aaatttattt
      541 gcaccaccgg taaactgccg gttccgtggc ctacgctcgt gaccacgttt ggttatggtg
      601 tagcatgctt ttcccgttac ccggaccaca tgaagcagca tgactttttt aaatccgcta
      661 tgccggaagg ttatgttcag gaacgcacca tcagctttaa aaacgacggt acgtacaaaa
      721 cgcgcgccga agtgaaattt gaaggtgata ccctcgttaa ccgcattgag ctgaagggca
      781 ttgatttcaa agaagatggt aatatccttg gtcataaact ggaatacaat ttcaattcgc
      841 acaacgtgta tattacggcc gataagcaga aaaatggtat taaggcaaat ttcaaaatcc
      901 gtcataacgt ggaagacggt tccgtccagc ttgctgatca ttaccagcag aacacgccga
      961 ttggcgatgg tccggtactg ctgcctgata accactacct ttctcaccag tcagcacttt
     1021 ccaaagatcc gaacgaaaaa cgcgatcata tggtactgct cgagtttgta acggccgcgg
     1081 gcatcaccca cggcatggac gaactttaca aataagtcga cggaggacag taatggccaa
     1141 gttgaccagt gccgttccgg tgctgaccgc acgtgacgtc gccggagcag tggagttctg
     1201 gaccgaccgt ctggggttct cccgtgactt cgtggaggac gacttcgccg gtgtggtccg
     1261 tgacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     1321 ggcctgggtg tgggtgcgtg gcctggacga gctgtacgcc gagtggagcg aagtcgtgtc
     1381 cacgaacttc cgtgacgcct ccgggccggc catgaccgag atcggcgagc agccatgggg
     1441 gcgtgagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     1501 ggactgaaag ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta
     1561 gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac
     1621 gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt
     1681 tagttcgtta aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg
     1741 agagcttagt acgtactatc aacaggttga actgccaacg actacgcact agccaacaag
     1801 agcttcaggg ttgagatgtg tataagagac agctgtctta atgaatcggc caacgcgcgg
     1861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2701 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2761 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     3841 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
